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Prevalence and antimicrobial susceptibility of Brachyspira species in pigs in Korea

  • Lim, Suk-Kyung (Animal, Plant, and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Hee-Soo (Animal, Plant, and Fisheries Quarantine and Inspection Agency) ;
  • Nam, Hyang-Mi (Animal, Plant, and Fisheries Quarantine and Inspection Agency) ;
  • Cho, Yun Sang (Animal, Plant, and Fisheries Quarantine and Inspection Agency) ;
  • Jung, Suk-Chan (Animal, Plant, and Fisheries Quarantine and Inspection Agency) ;
  • Joo, Yi-Seok (Animal, Plant, and Fisheries Quarantine and Inspection Agency)
  • 투고 : 2012.07.07
  • 심사 : 2012.10.31
  • 발행 : 2012.12.31

초록

The purpose of this study was to investigate the prevalence of Brachyspira species and antimicrobial susceptibility of Brachyspira (B.) hyodysenteriae isolates in Korea. A total of fifty-five Brachyspira species were isolated; five (1.0%) beta-hemolytic Brachyspira species and 50 (10.4%) weak hemolytic Brachyspira species from 116 different diarrheic pig samples and 367 apparently normal pig samples. In farm level, beta hemolytic and weak hemolytic Brachyspira species were detected in 7.4% (5/68) and 19.1% (13/68) of tested pig farms, respectively. By phenotypic and genotypic characterization, all beta hemolytic Brachyspira isolates was classified as group I (B. hyodysenteriae), whereas weak hemolytic Brachyspira species isolates were group III (B. innocens or B. murdochii). B. hyodysenteriae isolates showed high level of minimum inhibition concentrations to macrolide antimicrobials. This study shows that the prevalence of pathogenic B. hyodysenteriae in pigs is low but antimicrobial resistance of the pathogens is high in Korea. This is the first report of the prevalence of Brachyspira group III and antimicrobial susceptibility of B. hyodysenteriae in pigs in Korea. Our results could provide basic data for the management and treatment guidelines of Brachyspira infection.

키워드

참고문헌

  1. Atyeo RF, Stanton TB, Jensen NS, Suriyaarachichi DS, Hampson DJ. Differentiation of Serpulina species by NADH oxidase gene (nox) sequence comparisons and noxbased polymerase chain reaction tests. Vet Microbiol 1999, 67, 47-60. https://doi.org/10.1016/S0378-1135(99)00030-9
  2. BArcellos DESN, MAthiesen MR, de Uzeda M, Kader IITA, Duhamel GE. Prevalence of Brachyspira species isolated from diarrhoeic pigs in Brazil. Vet Rec 2000, 146, 398-403. https://doi.org/10.1136/vr.146.14.398
  3. Biksi I, Lorincz M, Molnar B, Kecskes T, Takacs N, Mirt D, Cizek A, Pejsak Z, Martineau GP, Sevin JL, Szenci O. Prevalence of selected enteropathogenic bacteria in Hungarian finishing pigs. Acta Vet Hung 2007, 55, 219-227. https://doi.org/10.1556/AVet.55.2007.2.8
  4. Buller NB, Hampson DJ. Antimicrobial susceptibility testing of Serpulina hyodysenteriae. Aust Vet J 1994, 71, 211-214. https://doi.org/10.1111/j.1751-0813.1994.tb03404.x
  5. Calderaro A, Merialdi G, Perini S, Ragni P, Guegan R, Dettori G, Chezzi C. A novel method for isolation of Brachyspira (Serpulina) hyodysenteriae from pigs with swine dysentery in Italy. Vet Microbiol 2001, 80, 47-52. https://doi.org/10.1016/S0378-1135(00)00374-6
  6. Fellstrom C, Pettersson B, Johansson KE, Lundeheim N, Gunnarsson A. Prevalence of Serpulina species in relation to diarrhea and feed medication in pig-rearing herds in Sweden. Am J Vet Res 1996, 57, 807-811.
  7. Fossi M, Saranpaa T, Rautiainen E. In vitro sensitivity of the swine Brachyspira species to tiamulin in Finland 1995- 1997. Acta Vet Scand 1999, 40, 355-358.
  8. Hidalgo A, Carvajal A, Garcia-Feliz C, Osorio J, Rubio P. Antimicrobial susceptibility testing of Spanish field isolates of Brachyspira hyodysenteriae. Res Vet Sci 2009, 87, 7-12. https://doi.org/10.1016/j.rvsc.2008.10.017
  9. Jensen TK, Christensen AS, Boye M. Brachyspira murdochii colitis in pigs. Vet Pathol 2010, 47, 334-338. https://doi.org/10.1177/0300985809359054
  10. Karlsson M, Aspan A, Landen A, Franklin A. Further characterization of porcine Brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin. J Med Microbiol 2004, 53, 281-285. https://doi.org/10.1099/jmm.0.05395-0
  11. Karlsson M, Fellstrom C, Gunnarsson A, Landen A, Franklin A. Antimicrobial susceptibility testing of porcine Brachyspira (Serpulina) species isolates. J Clin Microbiol 2003, 41, 2596-2604. https://doi.org/10.1128/JCM.41.6.2596-2604.2003
  12. Komarek V, MAderner A, Spergser J, Weissenbock H. Infections with weakly haemolytic Brachyspira species in pigs with miscellaneous chronic diseases. Vet Microbiol 2009, 134, 311-317. https://doi.org/10.1016/j.vetmic.2008.08.017
  13. La T, Phillips ND, Hampson DJ. Development of A duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces. J Clin Microbiol 2003, 41, 3372-3375. https://doi.org/10.1128/JCM.41.7.3372-3375.2003
  14. Lobova D, Smola J, Cizek A. Decreased susceptibility to tiamulin and valnemulin among Czech isolates of Brachyspira hyodysenteriae. J Med Microbiol 2004, 53, 287-291. https://doi.org/10.1099/jmm.0.05407-0
  15. Moller K, Jensen TK, Jorsal SE, Leser TD, Carstensen B. Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. Vet Microbiol 1998, 62, 59-72. https://doi.org/10.1016/S0378-1135(98)00199-0
  16. NAtional Antimicrobial Resistance Management Program. Monitoring of Antimicrobial Resistance on the Food- Animals And Meats. Korea Food And Drug AdministrAtion, Cheongwon, 2006.
  17. Sperling D, Smola J, izek A. Characterisation of multiresistAnt Brachyspira hyodysenteriae isolates from Czech pig farms. Vet Rec 2011, 168, 215. https://doi.org/10.1136/vr.c4247
  18. Stanton TB, Jensen NS. Purification and characterization of NADH oxidase from Serpulina (Treponema) hyodysenteriae. J Bacteriol 1993, 175, 2980-2987. https://doi.org/10.1128/jb.175.10.2980-2987.1993
  19. Stege H, Jensen TK, Moller K, Baekbo P, Jorsal SE. Prevalence of intestinal pathogens in Danish finishing pig herds. Prev Vet Med 2000, 46, 279-292. https://doi.org/10.1016/S0167-5877(00)00148-3
  20. Suh DK, Song JC. Prevalence of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella in swine herds. J Vet Sci 2005, 6, 289-293.
  21. ter Huurne AAHM, Gaastra W. Swine dysentery: more unknown than known. Vet Microbiol 1995, 46, 347-360. https://doi.org/10.1016/0378-1135(95)00049-G
  22. Thomson JR, Smith WJ, Murray BP, Murray D, Dick JE, Sumption KJ. Porcine enteric spirochete infections in the UK: surveillance data and preliminary investigation of atypical isolAtes. Anim Health Res Rev 2001, 2, 31-36.
  23. Uezato K, Kinjo E, Adachi Y. In vitro susceptibility of 21 antimicrobial agents to 37 isolates of Brachyspira hyodysenteriae isolated from pigs in Okinawa Prefecture. J Vet Med Sci 2004, 66, 307-309. https://doi.org/10.1292/jvms.66.307

피인용 문헌

  1. Antimicrobial Resistance in Clostridium and Brachyspira spp. and Other Anaerobes vol.8, pp.1, 2012, https://doi.org/10.1128/microbiolspec.arba-0020-2017