BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Mesodermal repression of single-minded in Drosophila embryo is mediated by a cluster of Snail-binding sites proximal to the early promoter

  • Park, Kye-Won (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Hong, Joung-Woo (Graduate School of East-West Medical Science, Kyung Hee University)
  • 투고 : 2012.05.14
  • 심사 : 2012.06.30
  • 발행 : 2012.10.31
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초록

single-minded (sim) is a master regulatory gene that directs differentiation in the central nervous system during Drosophila embryogenesis. Recent identification of the mesectoderm enhancer (MSE) of sim has led to the hypothesis that two Snail (Sna)-binding sites in the MSE may repress sim expression in the presumptive mesoderm. We provide evidence here that three Sna-binding sites proximal to the sim promoter, but not those of the MSE, are responsible for the mesodermal repression of sim in vivo. Using transgenic embryos injected with lacZ transgenes, we showed that sim repression in the mesoderm requires the three promoter-proximal Sna-binding sites. These results suggest that Sna represses the mesectodermal expression of sim by directly repressing the nearby promoter, and not by quenching adjacent transcriptional activators in the MSE. These data also showed how the MSE, lacking the three proximal Sna-binding sites, reproduced the endogenous pattern of sim expression in transgenic embryos.

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참고문헌

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피인용 문헌

  1. Discrete Levels of Twist Activity Are Required to Direct Distinct Cell Functions during Gastrulation and Somatic Myogenesis vol.9, pp.6, 2014, https://doi.org/10.1371/journal.pone.0099553
  2. An information theoretic treatment of sequence-to-expression modeling vol.14, pp.9, 2018, https://doi.org/10.1371/journal.pcbi.1006459