Journal of Plant Biotechnology

  • 제39권3호
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  • Pages.146-153
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  • 2012
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Antioxidant property of leaves and calluses extracts of in-vitro grown 5 different Ocimum species

  • Song, Hyuk (Department of Animal & Food Bioscience, Konkuk University) ;
  • Kumar, Prem (School of Biotechnology and Genetic Engineering, Bharathiar University) ;
  • Arivazhagan, Girija (School of Biotechnology and Genetic Engineering, Bharathiar University) ;
  • Lee, Sang-Il (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University) ;
  • Yoon, Hyung-Moon (Department of Orthopaedic Surgery, School of Mdedicine, Konkuk University) ;
  • Kim, Ick-Hee (Department of Surgery, School of Medicine, Konkuk University) ;
  • Kwon, Hyuk-Jung (Department of Family medicine, School of Medicine, Konkuk University) ;
  • Kim, Jong-Moon (Department of Rehabilitation, School of Medicine, Konkuk University) ;
  • Hakkim, Faruck Lukmanul (Department of Animal & Food Bioscience, Konkuk University)
  • 투고 : 2012.09.07
  • 심사 : 2012.09.14
  • 발행 : 2012.09.30
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초록

In this study, the antioxidant property of leaf and callus extracts of five selected in vitro grown Ocimum species (Ocimum sanctum, Ocimum kilimandscharicum, Ocimum gratissimum, Ocimum basilicum, and Ocimum americanum) and their respective callus extracts was investigated. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1mg L) combined with different concentrations (0.1-0.4 mg L) of kinetin as plant growth regulators. Total phenolic contents were estimated using the Folin-Ciocalteu reagent. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power, $Fe^{2+}$ chelating activity, and ${\beta}$-carotenelinoleic acid bleaching assays were used to determine the biological effects of the extracts. Interestingly, all the callus extracts exhibited significant (p<0.05) increase in phenolic contents and antioxidant activity. Furthermore, a liner correlation was obtained between the total phenolic contents and free radical scavenging activity ($R^2$ = 0.783). The extracts of leaves and calluses of Ocimum species exhibited activity in all the in vitro antioxidant assays, but its extent was less potent that the positive controls butylated hydroxyl anisole (BHA) and ascorbic acid. A higher accumulation of phenolics in the callus extracts suggests that isolation of high-concentration materials with antioxidant activivity is possible from in vitro callus cultures rather than field-grown plant organs. Furthermore, these extracts may be used as an effective preservative in the food industry.

키워드

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피인용 문헌

  1. Towards comprehension of complex chemical evolution and diversification of terpene and phenylpropanoid pathways in Ocimum species vol.5, pp.129, 2015, https://doi.org/10.1039/C5RA16637C
  2. Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation vol.252, pp.2, 2015, https://doi.org/10.1007/s00709-014-0711-3
  3. Ocimum basilicum suspension culture as resource for bioactive triterpenoids: yield enrichment by elicitation and bioreactor cultivation pp.1573-5044, 2019, https://doi.org/10.1007/s11240-018-01552-9