Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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The influence of silver thiosulfate and thidiazuron on shoot regeneration from cotyledon explants of Brassica napus

  • Roh, Kyung-Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kwak, Bo-Kyung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Jong-Bum (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Lee, Kyeong-Ryeol (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Hyun-Uk (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA) ;
  • Kim, Sun-Hee (Department of Agricultural Biotechnology, National Academy of Agricultural Science, RDA)
  • Received : 2012.09.03
  • Accepted : 2012.09.10
  • Published : 2012.09.30
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Abstract

The influences of ethylene inhibitors ($AgNO_3$ and silver thiosulfate) and cytokinins (BAP and TDZ) on shoot regeneration from cotyledon and hypocotyl explants of B. napus cv. Youngsan were investigated. The presence of $50{\mu}M$ Silver thiosulfate (STS) in shoot regeneration medium formed shoots at 60-68% after 3-4 weeks of culture, which was enhanced by 2-fold compared to that of Silver nitrate ($AgNO_3$). Moreover, cotyledon explants were more regenerative than hypocotyls; shoots from cotyledon explants began to occur 4-5 days earlier than that of hypocotyl explants. TDZ at a concentration of $8-10{\mu}M$ was effective for shoot regeneration, compared with BAP. Consequently, the optimal shoot regeneration response was observed in medium supplemented with $50{\mu}M\;STS+8{\mu}M\;TDZ$. In transmission electron microscopy (TEM) analysis, higher density of silver nanoparticles was shown to be accumulated widely inside the cell wall and plasmodesmata of regenerating leaf cultured in medium supplemented with $AgNO_3$. By contrast, in the cell cultured in medium with STS, fine-grained deposits were partly observed in the surroundings of the cell wall.

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References

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