Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Multivariate Analysis on 1H-NMR Spectroscopy of Olive Flounder Paralichthys olivaceus Serum

1H-NMR 스펙트럼의 다변량통계분석을 통한 넙치(Paralichthys olivaceus)의 백신 반응의 지표물질 분석

  • Cho, Ji-Young (Department of Marine Biotechnology, Soonchunhyang University)
  • 조지영 (순천향대학교 해양생명공학과)
  • Received : 2012.03.08
  • Accepted : 2012.08.03
  • Published : 2012.08.31
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Abstract

To investigate the relationship between metabolic changes in $^1H$-nuclear magnetic resonance (NMR) spectra and fish vaccination, serum was collected from olive flounders treated with a formalin-killed Edwardsiella tarda vaccine and used for $^1H$-NMR metabolite profiling. Principal component analysis and partial least squares were applied to the $^1H$-NMR profile to reduce its complexity and establish class-related clusters. Relative lipid regions were distinguished in vaccinated and non-vaccinated serum. Then, the lipids were extracted from the serum and analyzed. Triolein was identified.

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References

  1. Ala-Korpela M. 1995. $^{1}H$ NMR spectroscopy of human blood plasma. Prog Nucl Magn Reson Spectr 27, 475-554. https://doi.org/10.1016/0079-6565(95)01013-0
  2. Austin B and Austin DA. 1999. Characteristics of the diseases. In: Austin B, Austin DA (ed) Bacterial fish pathogens; diseases of farmed and wild fish, 3rd ed, Springer-Verlag, Heidelberg, Germany, 13-15.
  3. Brindle JT, Nicholson JK, Schofield PM, Grainger DJ and Holmes E. 2003. Application of chemometrics to $^{1}H$ NMR spectroscopic data to investigate a relationship between human serum metabolic profiles and hypertension. Analyst 128, 32-36. https://doi.org/10.1039/b209155k
  4. Castro N, Toranzo AE, Barja JL, Nunez S and Magarinos B. 2006. Characterization of Edwardsiella tarda strains isolated from turbot, Psetta maxima (L.). J Fish Dis 29, 541-547. https://doi.org/10.1111/j.1365-2761.2006.00750.x
  5. German JB, Hammock BD and Watkinsd SM. 2005. Metabolomics: building on a century of biochemistry to guide human health. Metabolomics 1, 3-9. https://doi.org/10.1007/s11306-005-1102-8
  6. Griffin JL and Kauppinen RA. 2007. A metabolomics perspective of human brain tumours. FEBS J 274, 1132-1139. https://doi.org/10.1111/j.1742-4658.2007.05676.x
  7. Jeon JK, Kim PK, Park YJ and Huh HT. 1995. Study of serum constituents in several species of cultured fish. J Korean Fish Soc 28, 123-130.
  8. Joo HJ and Cho JH. 2005. Pattern recognition using NMR spectral data for metabonomic analysis of urine samples from experimental animals. Yakhak Hoeji 49, 74-79.
  9. Kawai K, Liu Y, Ohnishi K and Oshima S. 2004. A conserved 37 kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate. Vaccine 22, 3411-3418. https://doi.org/10.1016/j.vaccine.2004.02.026
  10. Kim MS, Kim KD, Kim KW, Park MA and Kim JW. 2009. The efficacy and influence on growth of olive flounder Paralichthys olivaceus vaccinated against Edwardsiella tarda and Streptococcus iniae. J Fish Pathol 22, 327-334.
  11. Kim SW, Koo BC, Kim JH and Liu JR. 2006. Metabolic discrimination of rice cultivars and relative quatification of major sugar compounds using $^{1}H$ NMR spectroscopy combined by multivariate statistical analysis. J Plant Biotech 33, 283-288. https://doi.org/10.5010/JPB.2006.33.4.283
  12. Kori-Siakpere O, Ikomi RB and Ogbe MG. 2011. Biochemical response of the African catfish: Clarias gariepinus (Burchell, 1822) to sublethal concentrations of potassium permanganate. Annals Bio Res 2, 1-10.
  13. Liu Y, Oshima SI and Kawai K. 2007. Glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda has protective antigenicity against Vibrio anguillarum in Japanese flounder. Dis Aquat Org 75, 217-220. https://doi.org/10.3354/dao075217
  14. Massart DL and Vandeginste BGM. 1998. Chemometrics: a textbook. Elsevier Science Publishers, B.V., Amsterdam, Netherlands, 242.
  15. Metwally MAA. 2009. Effects of garlic (Allium sativum) on some antioxidant activities in Tilapia Nilotica (Oreochromis niloticus). World J Fish & Marine Sci 1, 56-64.
  16. Mohanty BR and Sahoo PK. 2007. Edwardsiellosis in fish: a brief review. J Biosci 32, 1331-1344. https://doi.org/10.1007/s12038-007-0143-8
  17. Nakatsugawa T. 1983. Edwardsiella tarda isolated from cultured young flounder. Fish Pathol 18, 99-101. https://doi.org/10.3147/jsfp.18.99
  18. Ramsewak RS, Nair MG, Murugesan S, Mattson WJ and Zasada J. 2001. Insecticidal fatty acids and triglycerides from Dirca palustris. J Agric Food Chem 49, 5852-5856. https://doi.org/10.1021/jf010806y