Mycobiology

The Korean Society of Mycology (한국균학회)
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Isolation and Characterization of the mheA (Most Highly Expressed) Gene of Aspergillus oryzae

  • Liu, Pengcheng (Department of Molecular Biology, Chonbuk National University) ;
  • Lim, Ji-Young (Department of Pharmaceutical Engineering, Woosuk University) ;
  • Kim, Hee-Seo (Department of Pharmaceutical Engineering, Woosuk University) ;
  • Kim, Jong-Hwa (Department of Pharmaceutical Engineering, Woosuk University) ;
  • Chae, Keon-Sang (Department of Molecular Biology, Chonbuk National University)
  • Received : 2012.08.21
  • Accepted : 2012.09.17
  • Published : 2012.09.30
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Abstract

The amino acid sequence of the mheA gene of Aspergillus oryzae encodes a putative metallothionein-like protein 1. The size of the mheA transcript was 497 nt and the mheA promoter was induced by glucose, consistent with results of analysis by Northern hybridization and with the pdcA promoter, respectively.

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References

  1. Nakajima K, Sano M, Machida M. Current progress in the analysis of transcriptional regulation in the industrially valuable microorganism Aspergillus oryzae. Biotechnol Bioprocess Eng 2000;5:253-62. https://doi.org/10.1007/BF02942182
  2. Hwang HA, Lee DW, Kim JH, Lee TK, Yang MS, Chae KS. Quantitative analysis of expressed genes in Aspergillus oryzae by sequencing 3'-directed cDNA clones. J Microbiol 1998;36:111-7.
  3. Okubo K, Hori N, Matoba R, Niiyama T, Fukushima A, Kojima Y, Matsubara K. Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nat Genet 1992;2:173-9. https://doi.org/10.1038/ng1192-173
  4. Lee DW, Koh JS, Kim JH, Chae KS. Cloning and nucleotide sequence of one of the most highly expressed genes, a pdcA homolog of Aspergillus nidulans, in Aspergillus oryzae. Biotechnol Lett 1999;21:139-42. https://doi.org/10.1023/A:1005438505623
  5. Maruyama IN, Rakow TL, Maruyama HI. cRACE: a simple method for identification of the 5' end of mRNAs. Nucleic Acids Res 1995;23:3796-7. https://doi.org/10.1093/nar/23.18.3796