Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Comparative Morphology of Minute Intestinal Fluke Eggs That Can Occur in Human Stools in the Republic of Korea

  • Lee, Jin-Joo (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Jung, Bong-Kwang (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Lim, Hye-Mi (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Lee, Mi-Youn (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Choi, Sung-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
  • Received : 2012.03.19
  • Accepted : 2012.06.06
  • Published : 2012.09.15
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Abstract

The egg morphology of minute intestinal flukes (MIF) that can occur as human infections in the Republic of Korea, i.e., Metagonimus yokogawai, M. miyatai, M. takahashii, Heterophyes nocens, Heterophyopsis continua, Stellantchasmus falcatus, Stictodora fuscata, Pygidiopsis summa, and Gymnophalloides seoi, was studied in comparison with Clonorchis sinensis. The adult worms were obtained from residents of endemic areas, and their intrauterine eggs were studied and measured using light microscopy; the length, width, length-width ratio (LWR), and Faust-Meleney index (FMI). Several specimens were processed for scanning electron microscopy (SEM), and before gold-coating, the uterine portion of each fluke was etched with a sharp pin in order to expose the eggs. The MIF eggs were ovoid, pyriform, or elliptical with a size range of $21-35{\times}12-21{\mu}m$. S. fuscata eggs revealed the highest FMI (largest in the area) and lowest LWR, whereas P. summa eggs showed the lowest FMI and medium LWR. SEM revealed that G. seoi and S. fuscata had remarkably clean shell surface lacking the muskmelon-like structure which is prominent in C. sinensis eggs. In Metagonimus spp., H. continua, H. nocens, and S. falcatus eggs, minute surface ridges were recognizable though less prominent compared with C. sinensis. On the surface of P. summa eggs, thread-like curly structures were characteristically seen. The results revealed that important differential keys for MIF eggs include the length, width, area (FMI), shape of the eggs, and the extent of the muskmelon-like structure or ridges on their shell surface and operculum.

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References

  1. Chai JY. Intestinal Flukes. In Murrell KD, Fried B eds, Food-borne Parasitic Zoonoses: Fish and Plant-borne Parasites. World Class Parasites. Vol. 11. New York, USA. Springer. 2007, p 53-115.
  2. Chai JY, Lee SH. Foodborne intestinal trematode infections in the Republic of Korea. Parasitol Int 2002; 51: 129-154. https://doi.org/10.1016/S1383-5769(02)00008-9
  3. Chai JY, Murrell KD, Lymbery AJ. Fish-borne parasitic zoonoses: Status and issues. Int J Parasitol 2005; 35: 1233-1254. https://doi.org/10.1016/j.ijpara.2005.07.013
  4. Cho SH, Cho PY, Lee DM, Kim TS, Kim IS, Hwang EJ, Na BK, Sohn WM. Epidemiological survey on the infection of intestinal flukes in residents of Muan-gun, Jeollanam-do, the Republic of Korea. Korean J Parasitol 2011; 48: 133-138. https://doi.org/10.3347/kjp.2010.48.2.133
  5. Lee SH, Hwang SW, Chai JY, Seo BS. Comparative morphology of eggs of heterophyids and Clonorchis sinensis causing human infections in Korea. Korean J Parasitol 1984; 22: 171-180. https://doi.org/10.3347/kjp.1984.22.2.171
  6. Chai JY, Shin EH, Lee SH, Rim HJ. Foodborne intestinal flukes in Southeast Asia. Korean J Parasitol 2009; 47(suppl): S69-S102. https://doi.org/10.3347/kjp.2009.47.S.S69
  7. Faust EC, Meleney HE. Studies on schistosomiasis japonica. Am J Hyg 1924; Monogr Ser no. 3: 1-339.
  8. Shin DH, Lim DS, Choi KJ, Oh CS, Kim MJ, Lee IS, Kim SB, Shin JE, Bok GD, Chai JY, Seo M. Scanning electron microscope study of ancient parasite eggs recovered from Korean mummies of the Joseon Dynasty. J Parasitol 2009; 95: 137-145. https://doi.org/10.1645/GE-1588.1
  9. Ishii Y. Scanning electron microscopical study on the structure of parasite eggs. Fukuoka Acta Med 1971; 63: 419-431.
  10. Ditrich O, Giboda M, Sterba J. Species determination of eggs of opisthorchiid and heterophyid flukes using scanning electron microscopy. Angew Parasitol 1990; 31: 3-9.
  11. Ditrich O, Giboda M, Scholz T, Beer SA. Comparative morphology of eggs of the Haplorchiinae (Trematoda: Heterophyidae) and some other medically important heterophyid and opisthorchiid flukes. Folia Parasitol (Praha) 1992; 39: 123-132.
  12. Kaewkes S, Elkins DB, Sithithaworn P, Haswell-Elkins MR. Comparative studies on the morphology of the eggs of Opisthorchis viverrini and lecithodendriid trematodes. Southeast Asian J Trop Med Public Health 1991; 22: 623-630.
  13. Tesana S, Srisawangwonk T, Kaewkes S, Sithithaworn P, Kanla P, Arunyanart C. Eggshell morphology of the small eggs of human trematodes in Thailand. Southeast Asian J Trop Med Public Health 1991; 22: 631-636.
  14. Sripa B, Bethony JM, Sithithaworn P, Kaewkes S, Mairiang E, Loukas A, Mulvenna J, Laha T, Hotez PJ, Brindley PJ. Opisthorchiasis and Opisthorchis-associated cholagiocarcinoma in Thailand and Laos. Acta Trop 2011; 120S: S158-S168.
  15. Sukontason K, Piangjai S, Sukontason K, Chaithong U. Potassium permanganate staining for differentiation the surface morphology of Opisthorchis viverrini, Haplorchis taichui and Phaneropsolus bonnei eggs. Southeast Asian J Trop Med Public Health 1999; 30: 371-374.

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