대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제38권3호
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  • Pages.237-245
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  • 2012
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지
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쿠메스트롤의 미토콘드리아 생합성 증가를 통한 피부 광노화 예방 효과

Beneficial Effect of Coumestrol on Ultraviolet B-Induced Skin Photoaging through Mitochondrial Biogenesis

  • 김수경 (아모레퍼시픽 기술연구원 뷰티푸드 연구소) ;
  • 김정기 (아모레퍼시픽 기술연구원 뷰티푸드 연구소) ;
  • 서대방 (아모레퍼시픽 기술연구원 뷰티푸드 연구소) ;
  • 이상준 (아모레퍼시픽 기술연구원 뷰티푸드 연구소)
  • Kim, Su-Kyung (Beauty Food Research Institute, Amorepacific Corporation R&D center) ;
  • Kim, Jeong-Kee (Beauty Food Research Institute, Amorepacific Corporation R&D center) ;
  • Seo, Dae-Bang (Beauty Food Research Institute, Amorepacific Corporation R&D center) ;
  • Lee, Sang-Jun (Beauty Food Research Institute, Amorepacific Corporation R&D center)
  • 투고 : 2012.05.04
  • 심사 : 2012.08.16
  • 발행 : 2012.09.30
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초록

쿠메스트롤은 식물이 스트레스에 대항해 합성하는 phytoalexins의 일종으로, 알팔파 새싹, 클로버, 콩나물에서 일반적으로 발견된다. 본 연구에서는 쿠메스트롤의 자외선에 의해 유도되는 피부 진피세포 광노화 예방 효능에 관한 연구를 실시하였다. 쿠메스트롤 전처리는 자외선 B 조사에 의해 감소된 Sirt1 단백질 발현 및 활성과 하위 미토콘드리아 생합성 관련 유전자인 PGC-$1{\alpha}$, NRF1, TFAM의 발현 변화를 감소시켰다. 또한, ATP 및 ROS 생성량을 정상화시키고 피부 노화를 유도하는 최종당화산물 생성을 억제하였다. 이상의 결과에서 쿠메스트롤은 자외선 조사에 의해 발생하는 진피 세포 내 미토콘드리아 손상 및 이에 따른 당화 단백질 생성을 감소시킴으로써 피부 광노화 현상으로부터 보호할 수 있음을 확인하였다.

Coumestrol is one of phytoalexins synthesized in response to environmental stress, and commonly found in natural foods such as alfalfa sprouts, clovers, and soybean. In the present study, we investigated the mechanism underlying protective effect of coumestrol against UVB-induced photoaging in human dermal fibroblasts. We found that pretreatment with coumestrol enhanced the UVB-suppressed mitochondrial biogenesis through regulation of Sirt1 expression and activity, and its downstream gene regulation such as PGC-$1{\alpha}$, NRF1, and TFAM. Moreover, the ATP and ROS production was restored to normal status and the formation of advanced glycation endproducts leading to skin photoaging in skin fibroblasts was blocked by coumestrol pretreatment before UVB irradiation. These findings indicate that coumestrol might potentially prevent skin photoaging induced by mitochondrial damage and glycated protein production in dermal fibroblasts.

키워드

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