International Journal of Oral Biology

  • 제37권3호
  • /
  • Pages.146-152
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  • 2012
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
권호 표지

MicroRNA Analysis during Cultured Odontoblast Differentiation

  • Park, Min-Gyeong (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Lee, Myoung-Hwa (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Yu, Sun-Kyoung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Park, Eu-Teum (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Seog (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Lee, Seul-Ah (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Moon, Yeon-Hee (Department of Dental hygiene, Chodang University) ;
  • Kim, Heung-Joong (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Chun-Sung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Do-Kyung (Oral Biology Research Institute, School of Dentistry, Chosun University)
  • 투고 : 2012.08.31
  • 심사 : 2012.09.11
  • 발행 : 2012.09.30
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초록

MicroRNAs (miRNAs, miRs) are about 21-25 nucleotides in length and regulate mRNA translation by base pairing to partially complementary sites, predominantly in the 3'-untranslated region (3'-UTR) of the target mRNA. In this study, the expression profile of miRNAs was compared and analyzed for the establishment of miRNA-related odontoblast differentiation using MDPC-23 cells derived from mouse dental papilla cells. To determine the expression profile of miRNAs during the differentiation of MDPC-23 cells, we employed miRNA microarray analysis, quantitative real-time PCR (qRT-PCR) and Alizaline red-S staining. In the miRNA microarray analysis, 11 miRNAs were found to be up- or down-regulated more than 3-fold between day 0 (control) and day 5 of MDPC-23 cell differentiation among the 1,769 miRNAs examined. In qRT-PCR analysis, the expression levels of two of these molecules, miR-194 and miR-126, were increased and decreased in the control MDPC-23 cells compared with the MDPC-23 cells at day 5 of differentiation, respectively. Importantly, the overexpression of miR-194 significantly accelerated mineralization compared with the control cultures during the differentiation of MDPC-23 cells. These results suggest that the miR-194 augments MDPC-23 cell differentiation, and potently accelerates the mineralization process. Moreover, these in vitro results show that different miRNAs are deregulated during the differentiation of MDPC-23 cells, suggesting the involvement of these genes in the differentiation and mineralization of odontoblasts.

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