Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Isolation of Bacteriophages Which Can Infect Pectobacteirum carotovorum subsp. carotovorum

Pectobacterium carotovorum subsp. carotovorum을 침해하는 박테리오파지의 분리

  • Jee, Sam-Nyu (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Malhotra, Shweta (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Roh, Eun-Jung (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jung, Kyu-Suk (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Dong-Whan (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Jae-Hyuk (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Yoon, Jong-Chul (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration) ;
  • Heu, Sung-Gi (Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration)
  • Received : 2012.06.18
  • Accepted : 2012.09.08
  • Published : 2012.09.30
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Abstract

Bacteriophages of Pectobacterium carotovorum subsp. carotovorum which causes soft rot on diverse vegetables had been isolated from 6 major Chinese cabbage cultivation areas in Korea. In order to isolate bacteriophages, total 15 different strains of P. carotovorum subsp. carotovorum isolated from nation-wide of Korea had been used as a host. When we tested 30 different soil samples individually from Pyeongchang and Taebaek with 15 different strains as a host, Taebek soil samples showed bacteriophage plaques with almost all different indicator strains but Pyeongchang soil samples showed plaques only with P. carotovorum subsp. carotovorum Pcc2 and Pcc3 strains. Especially, P. carotovorum subsp. carotovorum Pcc3 strain was able to produce plaques with almost all soil samples. Thus, this strain can be used as an indicator strain for P. carotovorum subsp. carotovorum bacteriophage screening. Electron microscope observation revealed P. carotovorum subsp. carotovorum bacteriophages isolated in Korea were belonged to three different families, Myoviridae, Siphoviridae and Podoviridae in order Caudovirales.

국내 주요 배추재배단지 6곳을 정하여 토양 샘플에서 무름병균을 용균할 수 있는 bacteriophage를 분리하였다. 여름배추를 재배하는 평창과 태백의 토양 샘플에서 국내에서 분리한 15개의 다른 무름병균을 기주로 파지를 분리한 결과 태백의 토양은 다양한 병원균을 기주로 증폭하는 반면 평창의 파지는 두 종류의 균에서만 증폭이 되어 매우 좁은 기주 범위를 가졌다. 무름병균 P. carotovorum subsp. carotovorum Pcc3의 균주는 거의 모든 토양 샘플에서 파지를 증폭할 수 있어 앞으로 파지를 이용한 무름병균 예찰 균주로 사용될 수 있는 가능성을 보여 주었다. 국내 무름병균을 용균할 수 있는 파지는 Myoviridae, Podoviridae, Siphoviridae 세 종류로 밝혀 졌으며 국내 거의 전 지역에서 Siphoviridae가 분리되었다.

Keywords

References

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