생명과학회지 (Journal of Life Science)

  • 제22권9호
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  • Pages.1201-1206
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Crlz1 promoter의 상위에 인접한 DNase I hypersensitive site 8의 enhancer 기능

DNase I Hypersensitive Site 8 as an Enhancer is Mapped in the Upstream Vicinity of the Crlz1 Promoter

  • 최승영 (경희대학교 생명과학대학 유전공학과 생명공학원) ;
  • 강창중 (경희대학교 생명과학대학 유전공학과 생명공학원)
  • Choi, Seong-Young (Department of Genetic Engineering, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University) ;
  • Kang, Chang-Joong (Department of Genetic Engineering, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
  • 투고 : 2012.06.15
  • 심사 : 2012.09.13
  • 발행 : 2012.09.30
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초록

Crlz1 유전자는 B 세포 분화 과정 중 pre-B 세포 분화 단계에서 특이적으로 발현됨이 밝혀져 있다. Crlz1 유전자의 발현과 연관되는 3개의 DNase I hypersensitive site (HSS8, 9, 10)가 Crlz1 유전자의 전사 시작점으로부터 바로 위쪽의 chromatin 상에서 발견되었고, 그 중에서 HSS9/10은 Crlz1 promoter 지역에 해당하며 매우 높은 전사 활성을 가지는 것으로 이미 보고 되었다. 본 연구에서는 앞의 연구에서 더 나아가 HSS8이 Crlz1 promoter의 전사활성을 약 2 배 증가시키는 enhancer의 역할을 하고 있는 현상을 밝혔으며, 또한 HSS8의 위치가 Crlz1 유전자의 전사 시작점을 기준으로 하였을 때 -1055와 -1159 사이, 즉 104 bp 길이의 chromatin DNA 상에 존재하며, 이러한 HSS8의 위치는 luciferase reporter의 활성 측정으로 비교 분석되어진 deletion construct들의 전사 활성에 대한 결과와도 잘 부합되었다.

The Crlz1 gene is known to be expressed specifically in the pre-B cell stage during B-cell development. With regards to the specific expression of the pre-B cell stage of the Crlz1 gene, we have previously identified three pre-B cell-specific DNase I hypersensitive sites (HSS), which are named HSS8, 9, and 10, in the upstream vicinity of this gene. In this paper, we report that HSS8 increases further the strong Crlz1 promoter activity driven by HSS9/10 and, therefore, acts as its enhancer. Furthermore, HSS8 has been finely mapped between -1055 and -1159 from the transcription start site of the Crlz1 gene.

키워드

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