Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Eupatorium chinensis var. simplicifolium Root Extract Inhibits the Lipopolysaccharide-Induced Inflammatory Response in Raw 264.7 Macrophages by Inhibiting iNOS and COX-2 Expression

Raw 264.7 대식세포에서 등골나물 뿌리 추출물의 염증반응 조절 분자 iNOS와 COX-2 발현 억제 효과

  • Lee, Jin-Ho (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Kim, Dae-Hyun (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Shin, Ji-Won (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Park, Sae-Jin (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
  • Kim, Yoon-Suk (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Shin, Yu-Su (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yu, Ji-Yeon (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology) ;
  • Kim, Tack-Joong (Division of Biological Science and Technology, College of Science and Technology, Yonsei University)
  • 이진호 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 김대현 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 신지원 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 박세진 (연세대학교 과학기술대학 생명과학기술학부) ;
  • 김윤석 (연세대학교 보건과학대학 임상병리학과) ;
  • 신유수 (농촌진흥청 국립원예특작과학원) ;
  • 유지연 (한국화학연구원) ;
  • 김택중 (연세대학교 과학기술대학 생명과학기술학부)
  • Received : 2012.07.08
  • Accepted : 2012.08.06
  • Published : 2012.09.30
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Abstract

Inflammation is a host defense mechanism that is activated in response to harmful substances or pathogens. However, an excessive inflammatory response is a problem in itself. Macrophages secrete inflammatory mediators such as nitric oxide (NO) or cytokines through various pathways such as the nuclear factor kappa B (NF-${\kappa}B$)-activated pathway after recognizing pathogen-like lipopolysaccharides (LPSs). In this study, anti-inflammatory effects of Eupatorium chinensis var. simplicifolium (EUC) extracts were investigated using LPS-stimulated RAW 264.7 macrophages. The EUC root extract significantly reduced NO production, inducible nitric oxide synthase (iNOS) expression, and cyclooxygenase-2 expression in a concentration-dependent manner. In addition, the EUC root extract reduced phosphorylation of mitogen-activated protein kinases and protein kinase B, which is upstream of NF-${\kappa}B$. The EUC root extract also reduced the degradation of inhibitory kappa B. These results indicate that EUC root extract exerts anti-inflammatory effects, which are mediated by inhibition of iNOS expression and the NF-${\kappa}B$ pathway.

염증반응은 유해한 물질이나 병원체에 대항하여 활성화되는 생체 방어 기전이다. 그러나 과도한 염증반응은 그 자체가 생체에 좋지 않은 영향을 미칠 수 있다. 대식세포는 지질다당류와 같은 병원체를 인식한 후, NF-${\kappa}B$ 경로의 활성화를 포함한 다양한 경로를 통하여 산화질소와 같은 염증매개인자들을 분비하는 면역세포이다. 본 연구에서는 지질다당류로 활성화시킨 RAW 264.7 대식세포를 이용하여 등골나물(Eupatorium chinensis var. simplicifolium) 뿌리, 줄기 그리고 꽃 추출물들의 항염증 효과를 알아보았다. 그 중 등골나물 뿌리의 추출물은 농도의존적으로 산화질소의 생성을 감소시켰으며, 산화질소 합성유도효소(inducible nitric oxide synthase)와 고리형 산소화효소-2(cyclooxygenase-2)의 발현을 통계적으로 유의하게 감소시켰다. 또한 등골나물 뿌리의 추출물은 NF-${\kappa}B$ 경로에 있는 MAP (mitogen activated protein) 인산화효소와 단백질 인산화효소 B (protein kinase B)의 활성화를 감소시켰으며, 억제적 kappa B (inhibitory kappa B)의 분해 또한 감소시키는 것을 관찰하였다. 이러한 결과는 등골나물 뿌리의 추출물이 NF-${\kappa}B$ 경로와 산화질소 합성유도효소 발현의 억제를 통하여 항염증작용을 나타낼 수 있음을 제시한다.

Keywords

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