Screening of Biological Activity of Caragana sinica Extracts

골담초(Caragana sinica) 추출물의 생리활성 탐색

In this study, extracts from Caragana sinica flowers and leaves were tested for antioxidant and angiotensin converting enzyme inhibitory activities, along with xanthine oxidase, tyrosinase, elastase, and astringent effects. Total phenolic compounds of acetone extracts from Caragana sinica flowers and leaves were the highest at 3.42 and 2.98 mg/g, respectively, when various extraction solvents were used. Optimal conditions for extraction of phenolic compounds from Caragana sinica leaves and flowers were 70% ethanol for 18 hr. DPPH scavenging activities were the highest in 70% ethanol extracts of Caragana sinica. ABTS radical cation decolorization values of 70% ethanol extracts were higher than those 60% ethanol extracts at 74%. Antioxidant protection factor was 1.2 PF in 70% ethanol extracts from Caragana sinica flowers and leaves. TBARS was lower than that of control (0.54 ${\mu}M$) in all sections. Angiotensin converting enzyme inhibitory activity of Caragana sinica flower extract was 80~90% at a phenolic concentration of 0.2~1.0 mg/mL, whereas xanthin oxidase inhibitory activity of Caragana sinica leaf extract was higher than that of flower extract. Tyrosinase inhibitory activity, which is related to skin-whitening, was above 20%, whereas elastase inhibitory activity related to anti-wrinkle effect was above 50% at a phenolic concentration of 0.8 mg/mL. Astringent effects of Caragana sinica flower and leaf extracts were higher than tannic acid as a control at an equivalent concentration. This result suggests that extracts from Caragana sinica flowers and leaves are suitable as functional foods having anti-hypertension, anti-gout, and medicinal cosmetic activities, including whitening and anti-wrinkle effects.

골담초에 함유된 페놀성 물질은 70% 에탄올을 용매로 하여 18시간 추출하였을 때 가장 많이 용출되었다. 항산화 효과 중 전자공여능을 측정한 결과는 꽃과 잎 모두 70% 에탄올 추출물에서 74%로 가장 높은 항산화 활성을 나타내었으며, ABTS radical cation decolorization을 측정한 결과, 꽃의 경우 70~90% 에탄올 추출물에서 60% 이상의 항산화 활성을 나타내었다. 지용성 물질에 대한 항산화력으로 antioxidant protection factor(PF)를 측정한 결과, 70% 에탄올 추출물에서 1.2 이상의 PF를 나타내었다. 활성산소 중 지방산화를 일으키는 hydroxyl radical에 대한 골담초 추출물의 영향은 꽃의 물 추출물과 70% 에탄올 추출물에서 대조구보다 낮은 TBARS 값을 나타내었다. ACE에 대한 골담초 추출물의 저해활성은 꽃의 경우 물과 70% 에탄올 추출물 모두 80~99%의 높은 저해활성을 나타내었고, Xoase 대한 저해활성은 꽃보다 잎 추출물의 저해활성이 높았다. 골담초 추출물의 주름개선과 미백효과를 확인한 결과 꽃과 잎 추출물 모두 페놀성 물질을 0.8 mg/mL의 농도로 처리하였을 때 50% 이상의 elastase 저해활성과 70% 에탄올 추출물에서 40.24%로 가장 높은 tyrosinase 저해활성을 나타내었고, 수렴효과는 같은 농도의 tannic acid보다 높은 수렴효과를 나타내었다.