The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Antibacterial Effects of Tunong-san and Kwaruwoobang-tang Aqueous Extracts against Staphylococcus aureus

투농산(透膿散) 및 과루우방탕(瓜蔞牛蒡湯)의 Staphylococcus aureus에 대한 In Vitro 항균력 평가

  • Jang, Se-Ran (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Daeguhaany University) ;
  • Park, Young-Sun (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Daeguhaany University) ;
  • Kim, Dong-Chul (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Daeguhaany University)
  • 장세란 (대구한의대학교 한의과대학 부인과교실) ;
  • 박영선 (대구한의대학교 한의과대학 부인과교실) ;
  • 김동철 (대구한의대학교 한의과대학 부인과교실)
  • Received : 2012.07.25
  • Accepted : 2012.08.16
  • Published : 2012.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: Recently Ciprofloxacin, used in the treatment of mastitis, showed many serious side effects. The object of this study was to recognize whether TNS and KWT can be used in the treatment of mastitis by observing the in vitro antibacterial effects of TNS and KWT aqueous herbal extracts against S. aureus. Methods: Antibacterial activities of TNS and KWT aqueous extracts against S. aureus ATCC 25923 were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were monitored at MIC and $MIC{\times}2$ levels. The effects on the intracellular killing and bacterial invasion of individual test materials were also observed using Raw 264.6 and MCF-7. The results were compared with Ciprofloxacin, a second generation of quinolone antibiotics in the present study. Results: MIC of aqueous extracts of TNS and KWT against S. aureus were detected as ($0.313{\pm}0.107$) and ($0.137{\pm}0.053$) mg/ml, respectively. MIC of Ciprofloxacin was detected as ($0.469{\pm}0.297$) ${\mu}g/ml$ at same conditions. In addition, TNS, KWT aqueous herbal extracts and Ciprofloxacin were also showed marked dosage-dependent inhibition of bacterial growth, and dramatical inhibitions on the both intracellular killing assays and bacterial invasion using Raw 264.6 and MCF-7 cells were detected. Conclusions: The results obtained in this study suggest that TNS and KWT aqueous herbal extracts showed antibacterial effects against S. aureus, and they also showed dosage-dependent inhibitory effects on the bacterial growth. And they showed the significant intracellular killing and bacterial invasion effects. It means, KWT and TNS may show more potent anti-infectious effects against S. aureus in vivo.

Keywords

References

  1. 정상설. 핵심유방학개론. 서울:고려의학. 1998:14-7.
  2. 김옥영. 여성 유방질환의 임상통계학적 고찰. 한국생화로가학연구원논총. 1989;14:91-7.
  3. 노동영 등. 양성유방질환의 임상적 역학적 연구. 대한외과학회지. 1993;44(6): 797-808.
  4. 한국유방암학회. 유방학. 서울:일조각. 1999:18-50.
  5. Peters F, Flick-Fillies D. Hand disinfection to prevent puerperal mastitis. Lancet. 1991;338(8770):831.
  6. Wambach KA. Lactation mastitis: a descriptive study of the experience. J Hum Lact. 2003;19(1):24-34. https://doi.org/10.1177/0890334402239731
  7. Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987; 51(1):88-134.
  8. Okamoto R et al. Detection of genes regulating beta-lactamase production in Enterococcus faecalis and Staphylococcus aureus. Antimicrob Agents Chemother. 1996;40(11):2550-4.
  9. Ackermann G et al. Resistant to moxifloxacin in toxigenic Clostridium difficile isolates is associated with mutations in gyrA. Antimicrob Agents Chemother. 2001;45(8):2348-53. https://doi.org/10.1128/AAC.45.8.2348-2353.2001
  10. Akasaka T et al. Type II topoisomerase mutations in fluoroquinolone-resistant clinical strains of Pseudomonas aeruginosa isolated in 1998 and 1999: role of target enzyme in mechanism of fluoroquinolone resistance. Antimicrob Agents Chemother. 2001;45(8):2263-8. https://doi.org/10.1128/AAC.45.8.2263-2268.2001
  11. Bachoual R et al. Single or double mutation alterations of gyrA associated with fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli. Microb Drug Resist. 2001;7(3): 257-61. https://doi.org/10.1089/10766290152652800
  12. 한의부인과학 교재편찬위원회. 한방 여성의학 II. 서울:정담. 2007:446-9.
  13. Pfaller MA et al. Multicenter evaluation of four methods of yeast inoculum preparation. J Clin Microbiol. 1988; 26(8):1437-41.
  14. Janssen AM et al. Antimicrobial activity of essential oils: a 1976-1986 literature review. Aspects of the test methods. Planta Med. 1987;53(5): 395-8. https://doi.org/10.1055/s-2006-962755
  15. Cuffini AM et al. Enhanced Staphylococcus aureus susceptibility to immuno-defense induced by sub-inhibitory and bactericidal concentration of imipenem. J Antimicrob Chemotherp. 1993;31(4):559-68. https://doi.org/10.1093/jac/31.4.559
  16. Almeida RA et al. Staphylococcus aureus invasion of bovine mammary epithelial cells. J Dairy Sci. 1996; 79(6):1021-6. https://doi.org/10.3168/jds.S0022-0302(96)76454-8
  17. Menzies BE, Kourteva I. Internalisation of Staphylococcus aureus by endothelial cells induces apoptosis. Infect Immunol. 1998;66(12):5994-8.
  18. 신혁재. 유선염의 최신지견. 의약정보. 2011;37(1):22-7.
  19. 대한산부인과학회. 산과학. 4판. 서울: 군자출판사. 2007:490.
  20. 배종국. 한방유방학. 서울:정담. 2005: 153-81.
  21. Medeiros AA. Beta-lactamases. Br Med Bull. 1984;40(1):18-27. https://doi.org/10.1093/oxfordjournals.bmb.a071942
  22. Appelbaum PC, Hunter PA. The fluoroquinolone antibacterials: past, present and future perspectives. Int J Antimicrob Agents. 2000;16(1):5-15. https://doi.org/10.1016/S0924-8579(00)00192-8
  23. Pispirigos K, Chrysanthopoulos K. Evaluation of cardiac subacutetoxicity of ciprofloxacin in rats using serum biochemical parameters. Arzneimittelforschung. 2001;51(7):582-7.
  24. Pons R, Escutia B. Ciprofloxacin-induced vasculitis with cutaneous and renal involvment. Nefrologia. 2001;21(2):209-12.
  25. Egerbacher M et al. Effects of enrofloxacin and ciprofloxacin hydrochloride on canine and equine chondrocytes in culture. Am J Vet Res. 2001;62(5):704-8. https://doi.org/10.2460/ajvr.2001.62.704
  26. Williams RJ 3rd et al. The effect of ciprofloxacin on tendon, paratenon, and capsular fibroblast metabolism. Am J Sports Med. 2000;28(3):364-9. https://doi.org/10.1177/03635465000280031401
  27. 許浚. 東醫寶鑑. 하동:동의보감출판사. 2005:679-89.
  28. 陳自明. 婦人大全良方. 北京:人民衛生出版社. 1985:642-51.
  29. 葉天士. 葉天士女科. 서울:대성문화사. 1984:300-2.
  30. 武之望. 濟陰綱目. 台北:旅風出版社. 1977:557.
  31. 陳實功. 外科正宗. 北京:人民衛生出版社. 1983:1-21, 33.
  32. 中醫歷代名方集成. 上海:上海辭書出版社. 1994:1014-5.
  33. 許沛群 外. 方劑學. 北京:人民衛生出版社. 1995:595-6.
  34. 吳謙 等編. 醫宗金鑑. 北京:人民衛生出版社. 1982:1613, 1781.
  35. 박미형. 유옹에 활용되는 내소산의 진통소염작용에 대한 연구. 대전대학교대학원. 1996.
  36. 김연희. 유용에 활용되는 과루우방탕이 소염 진통 작용에 미치는 영향. 대전대학교대학원. 1992.
  37. 강동환. 투유에 활용되는 가미연교탕이 소염 진통 및 항균작용에 미치는 영향. 대전대학교대학원. 1992.
  38. 백성준. 유옹에 활용되는 가미소독음 의 진통소염작용에 대한 연구. 대한한방부인과학회지. 1997;10(1):51-61.
  39. 김정숙. 유옹에 활용되는 가감류기음에 대한 실험적 연구. 대전대학교 대학원. 1995.
  40. Seral C et al. Quantitative analysis of gentamicin, azithromycin, telithromycin, ciprofloxacin, moxifloxacin, and oritavancin (LY333328) activities against intracellular Staphylococcus aureus in mouse J774 macrophages. Antimicrob Agents Chemother. 2003;47(7):2283-92. https://doi.org/10.1128/AAC.47.7.2283-2292.2003
  41. Gresham HD et al. Survival of Staphylococcus aureus inside neutrophils contributes to infection. J Immunol. 2000;164(7):3713-22. https://doi.org/10.4049/jimmunol.164.7.3713
  42. Maurin M, Raoult D. Use of aminoglycosides in treatment of infections due to intracellular bacteria. Antimicrob Agents Chemother. 2001; 45(11):2977-86. https://doi.org/10.1128/AAC.45.11.2977-2986.2001
  43. Diarra MS et al. Lactoferrin against Staphylococcus aureus Mastitis. Lactoferrin alone or in combination with penicillin G on bovine polymorphonuclear function and mammary epithelial cells colonisation by Staphylococcus aureus. Vet Immunol Immunopathol. 2003;95(1-2):33-42. https://doi.org/10.1016/S0165-2427(03)00098-9
  44. Navarre WW, Schneewind O. Surface proteins ofgram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol Mol Biol Rev. 1999;63(1):174-229.