The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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The Antiinflammatory Effects of Chaenomelis Fructus Herba Water Extract on Mouse RAW 264.7 Cell

모과(木瓜) 물추출물의 항염증 효능에 관한 실험적 연구

  • Ryu, Hahn-Woo (Dept. of Gynecology, College of Oriental Medicine, Ga-Chon University) ;
  • Kim, Yoon-Sang (Dept. of Gynecology, College of Oriental Medicine, Ga-Chon University) ;
  • Lim, Eun-Mee (Dept. of Gynecology, College of Oriental Medicine, Ga-Chon University)
  • 류한우 (가천대학교 한의과대학 한방부인과교실) ;
  • 김윤상 (가천대학교 한의과대학 한방부인과교실) ;
  • 임은미 (가천대학교 한의과대학 한방부인과교실)
  • Received : 2012.07.16
  • Accepted : 2012.08.16
  • Published : 2012.08.31
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Abstract

Objectives: The purpose of this study was to investigate the effects of Chaenomelis Fructus Herba Water Extract(CF) on the production of inflammatory mediators in RAW 264.7 cell mouse macrophages stimulated with LPS. Methods: We have not examined effect of CF on the cell viability of RAW 264.7 cell until we investigated effects of CF on LPS-induced productions of NO, Ca and various cytokines in RAW 264.7 cell. And when p-value is below 0.05, it is judged to have the significant difference statistically(P<0.05). Results: 1. CF increased the cell viability in the RAW 264.7 cell at the density of 25, 50, 100 and 200 ${\mu}g/ml$. 2. CF inhibited significantly increasing the production of NO in LPS-induced RAW 264.7 cell at the density of 25, 50, 100 and 200 ${\mu}g/ml$. 3. CF inhibited significantly increasing the production of Intracellular Ca in LPS-induced RAW 264.7 cell at the density of 25, 50, 100 and 200 ${\mu}g/ml$. 4. CF inhibited significantly the IL-2, IL-10, IL-12p70, TNF-${\alpha}$, GM-CSF, M-CSF, LIF and VEGF of the RAW 264.7 cell induced by LPS at the density of 25, 50, 100 and 200 ${\mu}g/ml$. 5. CF inhibited significantly the IL-4 at the density of 25, 50 ${\mu}g/ml$, the IL-5, IL-15 and MIG at the density of 25, 50 and 200 ${\mu}g/ml$ and IFN-${\gamma}$ at the density of 25, 100 ${\mu}g/ml$ respectively in the RAW 264.7 cell increased by LPS. Conclusions: CF inhibited significantly increasing IL-2, IL-10, IL-12p70, TNF-${\alpha}$, GM-CSF, M-CSF, LIF, VEGF, NO and Ca in LPS-induced RAW 264.7 cell at the density of more than 25 ${\mu}g/ml$ without causing the toxicity. These results signify that CF has antiinflammatory effect on controlling the over inflammatory reaction by the RAW 264.7 cell.

Keywords

References

  1. 한의부인과학 편찬위원회. 한의부인과학. 서울:정담. 2004:167-204, 260-87.
  2. Porpora MG et al. Celiac disease as a cause of chronic pelvic pain, dysmenorrhea, and deep dyspareunia. Obstet Gynecol. 2002;99(5 Pt 2): 937-9.
  3. Moore J, Kennedy S. Causes of chronic pelvic pain. Baillieres Best Pract Res Clin Obstet Gynaecol. 2000;14(3): 389-402. https://doi.org/10.1053/beog.1999.0082
  4. 김동일, 이동규, 이태균. 만성골반통을 유발하는 골반울혈증후군의 임상에 관 한 연구. 대한한방부인과학회지. 2002; 15(2):115-24.
  5. 조형준, 이진용, 김덕곤. 상백피가 항알러지 및 항염증반응에 미치는 영향. 대한한방소아과학회지. 2005;19(2):175-95.
  6. 유태무 등. 천연물의 항염증작용에 관한 연구. 응용약물학회지. 1998;6:269-75.
  7. 韓醫科大學 本草學 編纂委員會 編著. 本草學. 서울:영림사. 1991:272-73.
  8. 이수정 등. 木瓜의 抗炎 및 免役反應 에 대한 實驗的 硏究. 대한한방부인과학회지. 2008;21(4):36-48.
  9. 이태진 등. 木瓜, 虎杖根 및 乳香 추출물이 Raw 264.7cell에서 LPS로 유도된 nitricoxide 생성에 미치는 영향. 동의생리병리학회지. 2006;20(3):603-8.
  10. 이태진 등. 木瓜, 虎杖根 및 乳香 추출물이 Raw 264.7cell에서 LPS로 유도된 MMP-9의 활성에 미치는 영향. 동의생리병리학회지. 2006;20(1):37-42.
  11. Anderson GP, Lamar JD, Charles PT. Development of a Luminex based competitive immunoassay for 2,4,6-trinitrotoluene. Environ Sci Technol. 2007;41(8):2888-93. https://doi.org/10.1021/es062333n
  12. Djoba Siawaya JF et al. An evaluation of commercial fluorescent bead-based luminex cytokine assays. PLoS One. 2008;3(7):2535. https://doi.org/10.1371/journal.pone.0002535
  13. Dupont NC et al. Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: determinations of a panel of nine cytokines in clinical sample culture superna tants. J Reprod Immunol. 2005;66(2):175-91. https://doi.org/10.1016/j.jri.2005.03.005
  14. Khan SS et al. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers. Cytometry B Clin Cytom. 2004;61(1):35-9.
  15. 해리슨 번역 편찬위원회. 해리슨 내과학. 서울:정담. 1997:2188-208.
  16. 대한병리학회. 병리학. 서울:고문사. 1995:71-104.
  17. Abbas AK, Lichtman AH, Pober JS. Cellular and Molecular Immunology. philadelphia:WBSaunders company. 1997:11, 25-26, 250-64, 397.
  18. 김창종, 서병세. 최신 일반병리학(4th). 서울:신일상사. 2004:145-68.
  19. Gleicher N, Barad DH. Gender as risk factor for autoimmune diseases. J Autoimmun. 2007;28(1):1-6. https://doi.org/10.1016/j.jaut.2006.12.004
  20. 채우석. 免疫疾患의 韓方槪念과 治療에 관한 文獻的 考察. 대한한의학회지. 1990;11(2):54-91.
  21. 임은미, 이시형. 한의학에서 살펴본 면역의 개념-원전을 통한 면역개념의 존재와 확인(면역과 한방의 재정리). 대한한의정보학회지. 2006;12(1): 129-45.
  22. 홍원식. 精校黃帝內經. 서울:東洋醫學硏究院. 1981:13, 69, 318.
  23. 손수곤, 김종수, 박원환. 腎兪穴의 鍼刺戟과 黃芪藥鍼이 실험용 생쥐의 免疫活性物質 IL-$1{\beta}$의 遺傳子發顯에 미치는 影響. 대한한의학회지. 2000; 21(4):16-25.
  24. 정연희, 유동렬. 加味補兒湯의 免疫 調節作用에 대한 實驗的 硏究. 대한한방소아과학회지. 2000;14(2):61-83.
  25. 송채석, 박은정, 정규만. 地楡湯加 枳實이 생쥐의 免疫反應에 미치는 影響. 대한한방소아과학회지. 1992;6(1):15-32.
  26. 이승연, 김장현. 蔘朮健脾湯이 흰쥐의 免疫反應에 미치는 影響. 대한한방소아과학회지. 1988;12(1):257-76.
  27. 나도균, 박양춘. 加味地黃湯이 천식 모델 생쥐의 면역세포 및 사이토카 인에 미치는 영향. 대한한의학회지. 2008;29(2):7-20.
  28. 송종석 등. 加味補中益氣湯이 생쥐의 免疫反應에 미치는 影響. 대한한방부인과학회지. 2006;19(4):1-16.
  29. 이병렬, 안병철. 四君子湯 및 四物湯 藥鍼이 免疫反應에 미치는 影響. 대한한의학회지. 1997;18(1):357-74.
  30. 임은미. 여성본초학. 서울:전국의학사. 2005:131-2.
  31. Lehnardt S, Massillon L, Follett P. Activation of innate immunity in the CNS triggers neurodegeneration through a Toll-like receptor 4-dependent pathway. Proc Natl Acad Sci USA. 2003;100(14):8514-9. https://doi.org/10.1073/pnas.1432609100
  32. Martin L et al. Activation of the adenosine A3 receptor in RAW 264.7 cells inhibits lipopolysaccharide-stimulated tumor necrosis factor-alpha release by reducing calcium-dependent activation of nuclear factor-kappaB and extracellular signal-regulated kinase 1/2. J Pharmacol Exp Ther. 2006; 316(1):71-8.
  33. Salgame P et al. Differing lymphokine profiles of functional sunsets of human CD4 and CD8 T cell clones. Science. 1991;254(5029):279-82. https://doi.org/10.1126/science.1681588
  34. Mahanty S et al. Parallelregulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148(11): 3567-71.
  35. Samua D, Michea P, Lennon-Dumenil AM. Interleukin-4 Selectively Inhibits Interleukin-2 Secretion by Lipopolysaccharide-Activated Dendritic Cells. Scandinavian Journal of Immunology. 2004;59(2):183-9. https://doi.org/10.1111/j.0300-9475.2004.01380.x
  36. 김윤희. 사이토카인의 종류와 기능에 관한 연구. 전주기전여자대학논문집. 2000;20:271-85.
  37. Goldsby RA et al. Immunology 5th ed. New York:WH Freeman and Company. 2005:281.
  38. Trinchieri G. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol. 2003;3(2):133-46. https://doi.org/10.1038/nri1001
  39. Geijsen N, Koenderman L, Coffer PJ. Specificity in cytokine signal transduction: lessons learned from the IL-3/IL-5/GM-CSF receptor family. Cytokine Growth Factor Rev. 2001;12(1):19-25. https://doi.org/10.1016/S1359-6101(00)00019-8
  40. Doyle SE, Gasson JC. Characterization of the role of the human gr anulocyte -macrophage colony-stimulating factor receptor alpha sub unit in the activation of JAK2 and STAT5. Blood. 1998;92(3):867-76.
  41. Osdoby P et al. Osteoclast development the cell surface and the bone environment. Ciba Found Symp. 1988;136:108-24.
  42. 고승희, 황정혜, 장세진. 황체기 결함이 있는 불임환자에게 클로미펜 투여 후 자궁내막에서의 Leukemia inhibitory factor (LIF)의 발현. 대한산부인과학회지. 2003;46(11):2221-6.
  43. Guidi AJ et al. Vascular permeability fact or (vascular endothelial growth factor) expression and angiogenesis in cervical neoplasia. J Natl Cancer Inst. 1995;87(16):1237-45. https://doi.org/10.1093/jnci/87.16.1237
  44. Olver S et al. The duplicitous effects of interleukin 4 on tumour immunity: how can the same cytokine improve or impair control of tumour growth? Tissue Antigens. 2007;69(4):293-8. https://doi.org/10.1111/j.1399-0039.2007.00831.x
  45. Sender RA, Paul WE. Acquisition of lymphokine-producting phenotype by CD4+ T cells. Annu Rev Immunol. 1994;12:635-73. https://doi.org/10.1146/annurev.iy.12.040194.003223
  46. van Driel WJ et al. Presence of an eosinophilic infiltrate in cervical squamous carcinoma results from a type 2 immune response. Gynecol Oncol. 1999;74(2):188-95. https://doi.org/10.1006/gyno.1999.5431
  47. Bamford RN et al. The interleukin (IL) 2 receptor beta chain is shared by IL-2 and cytokine, provisionally designated IL-T, that stimulates T-cell proliferation and the induction of lymphokine-activated killer cells. Proc Natl Acad Sci USA. 1994;91(11): 4940-4. https://doi.org/10.1073/pnas.91.11.4940
  48. Farber JM. HuMIG: a new human member of the chemokine family of cytokines. Biochem Biophys Res Commun. 1993;192(1):223-30. https://doi.org/10.1006/bbrc.1993.1403
  49. Sypek JP et al. Ressolution of cutaneous lei shmaniasis: interleukin 12 initiates a protective T helper type 1 immune response. J Exp Med. 1993;177(6): 1797-802. https://doi.org/10.1084/jem.177.6.1797
  50. Albina JE et al. Nitric oxide-mediated apoptosis in murine peritoneal macrophage. J Immunol. 1993;150(11): 5080-5.
  51. Fehsel K et al. Nitric oxide induces apoptosis in mouse thymocytes. J Immunol. 1995;155(6):2858-65.

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