Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Fagopyrum esculentum Extract Suppresses the Release of Inflammatory Mediator and Proximal Signal Events in $Fc{\varepsilon}RI$-mediated RBL-2H3 Cell Activation

교맥(蕎麥)의 비만세포 염증매개물질의 분비와 $Fc{\varepsilon}RI$ 신호전달에 미치는 효과

  • Kang, Kyung-Hwa (Department of Oriental Physiology, College of Oriental Medicine, Dongeui University)
  • 강경화 (동의대학교 한의과대학 생리학교실)
  • Received : 2012.07.16
  • Accepted : 2012.07.31
  • Published : 2012.08.25
Download PDF
⟨ Previous Next ⟩

Abstract

Fagopyrum esculentum(FE) is an important food crop and medicinal plant that is used to improve diabetes, obesity, hypertension, hypercholesterolemia and constipation in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We investigated the effects on the release of inflammatory mediator and proximal signal events in $Fc{\varepsilon}RI$-mediated RBL-2H3 cell activation. FE reduced antigen (DNP-HSA)-induced release of histamine, prostaglandin D2 (PGD2) and cysteinyl Leukotriene (cysLT) in IgE-sensitized RBL-2H3 cells. In addition, it inhibited antigen-induced HDC2 and COX-2 and 5-LO mRNA expression in IgE-sensitized RBL-2H3 cells. FE also suppressed antigen-induced $Fc{\varepsilon}RI{\beta}$ and $Fc{\varepsilon}RI{\gamma}$ subunit mRNA expression in these cells. To identify the mechanisms underpinning the inhibition of release of inflammatory mediators such as histamine and PGD2 and cysLT by FE, we examined the proximal signal events of intracellular FceRI signaling molecules. FE suppressed antigen-induced phosphorylation of Lyn, Syk, LAT, $PLC{\gamma}1$, PI3K, Akt and cPLA2. Collectively, the anti-allergic effects of FE in vitro suggest its possible therapeutic application to inflammatory allergic diseases, in which its inhibition of inflammatory mediator and FceRI-dependent signaling events in mast cells may be hugely beneficial.

Keywords

References

  1. Metcalfe, D.D., Baram, D., Mekori, Y.A. Mast cells. Physiol Rev 77(4):1033-1079, 1997.
  2. Gilfillan, A.M., Rivera, J. The tyrosine kinase network regulating mast cell activation. Immunol Rev 228(1):149-169, 2009. https://doi.org/10.1111/j.1600-065X.2008.00742.x
  3. 허 준. (신대역) 동의보감. 서울, 법인문화사, p 1808, 2007.
  4. 중약대사전 편찬위원회. 중약대사전. 서울, 정담, pp 392-393, 2006.
  5. Hur, S.J., Park, S.J., Jeong, C.H. Effect of buckwheat extract on the antioxidant activity of lipid in mouse brain and its structural change during in vitro human digestion. J. Agric. Food Chem. 59(19):10699-10704, 2011. https://doi.org/10.1021/jf202279r
  6. van den Berg, A.J.J., van den Worm, E., van Ufford, H.C.Q., Halkes, S.B.A., Hoekstra, M.J., Beukelman, C.J. An in vitro examination of the antioxidant and anti-inflammatory properties of buckwheat honey. J Wound Care 17(4):172-174, 176-178, 2008.
  7. Ishii, S., Katsumura, T., Shiozuka, C., Ooyauchi, K., Kawasaki, K., Takigawa, S., Fukushima, T., et al. Anti-Inflammatory Effect of Buckwheat Sprouts in Lipopolysaccharide-Activated Human Colon Cancer Cells and Mice. Bioscience, Biotechnology, and Biochemistry 72(12):3148-3157, 2008. https://doi.org/10.1271/bbb.80324
  8. Li, S.Q., Zhang, Q.H. Advances in the development of functional foods from buckwheat. Crit Rev Food Sci Nutr 41(6):451-464, 2001. https://doi.org/10.1080/20014091091887
  9. Lee, C.C., Hsu, W.H., Shen, S.R., Cheng, Y.H., Wu, S.C. Fagopyrum tataricum (Buckwheat) Improved High-Glucose-Induced Insulin Resistance in Mouse Hepatocytes and Diabetes in Fructose-Rich Diet-Induced Mice. Exp Diabetes Res 2012: 2012.
  10. No, S.T., Kim, D.S., Lee, S.J., Park, D.J., Lee, J.C., Lim, K.S., Woo, W.H., M, Y.J. Study of Skin Depigmenting Mechanism of the Ethanol Extract of Fagopyrum esculentum. Korean J. Oriental Physiology & Pathology, 21(5):1243-1249, 2007.
  11. Fukui, H. Progress in Allergy Signal Research on Mast Cells: Up-Regulation of Histamine Signal-Related Gene Expression in Allergy Model Rats. Journal of Pharmacological Sciences 106(3):325-331, 2008. https://doi.org/10.1254/jphs.FM0070184
  12. Murphy, R.C., GijOn, M.A. Biosynthesis and metabolism of leukotrienes. Biochemical Journal 405(3):379, 2007. https://doi.org/10.1042/BJ20070289
  13. Boyce, J.A. Mast cells and eicosanoid mediators: a system of reciprocal paracrine and autocrine regulation. Immunological Reviews 217(1):168-185, 2007. https://doi.org/10.1111/j.1600-065X.2007.00512.x
  14. Tanaka, K., Ogawa, K., Sugamura, K., Nakamura, M., Takano, S., Nagata, K. Cutting Edge: Differential Production of Prostaglandin D2 by Human Helper T Cell Subsets. J Immunol 164(5):2277-2280, 2000.
  15. Westcott, J.Y., Wenzel, S.E., Dreskin, S.C. Arachidonate-induced eicosanoid synthesis in RBL-2H3 cells: stimulation with antigen or A23187 induces prolonged activation of 5-lipoxygenase. Biochim. Biophys. Acta 1303(1):74-81, 1996. https://doi.org/10.1016/0005-2760(96)00074-4
  16. Blank, U., Ra, C., Miller, L., White, K., Metzger, H., Kinet, J.P. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 337(6203):187-189, 1989. https://doi.org/10.1038/337187a0
  17. Ra, C., Jouvin, M.H., Kinet, J.P. Complete structure of the mouse mast cell receptor for IgE (Fc epsilon RI) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells. J. Biol. Chem. 264(26):15323-15327, 1989.
  18. Ravetch, J.V., Kinet, J.P. Fc Receptors. Annual Review of Immunology 9(1):457-492, 1991. https://doi.org/10.1146/annurev.iy.09.040191.002325
  19. Blank, U., Ra, C.S., Kinet, J.P. Characterization of truncated alpha chain products from human, rat, and mouse high affinity receptor for immunoglobulin E. J. Biol. Chem. 266(4):2639-2646, 1991.
  20. Lin, S., Cicala, C., Scharenberg, A.M., Kinet, J.P. The FceRI$\beta$ Subunit Functions as an Amplifier of FceRI$\gamma$-Mediated Cell Activation Signals. Cell 85(7):985-995, 1996. https://doi.org/10.1016/S0092-8674(00)81300-8
  21. Kinet, J.P. The high-affinity IgE receptor (Fc epsilon RI): from physiology to pathology. Annu. Rev. Immunol. 17: 931-972, 1999. https://doi.org/10.1146/annurev.immunol.17.1.931
  22. Zhang, J., Billingsley, M.L., Kincaid, R.L., Siraganian, R.P. Phosphorylation of Syk Activation Loop Tyrosines Is Essential for Syk Function AN IN VIVO STUDY USING A SPECIFIC ANTI-Syk ACTIVATION LOOP PHOSPHOTYROSINE ANTIBODY. J. Biol. Chem. 275(45):35442-35447, 2000. https://doi.org/10.1074/jbc.M004549200
  23. Kalesnikoff, J. Galli, S.J. New developments in mast cell biology. Nat Immunol 9(11):1215-1223, 2008. https://doi.org/10.1038/ni.f.216
  24. Fukao, T., Terauchi, Y., Kadowaki, T., Koyasu, S. Role of phosphoinositide 3-kinase signaling in mast cells: new insights from knockout mouse studies. J. Mol. Med. 81(9):524-535, 2003. https://doi.org/10.1007/s00109-003-0475-2