Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study on the Immune Modulatory Activity of Seokjahaeki-tang Using Atopic Dermatitis Animal Models

아토피피부염 동물 병태 모델에서 석자해기탕(石紫解肌湯)의 면역조절작용에 관한 연구

  • Lee, Byung-Woong (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Gim, Seon-Bin (Traditional and Biomedical Research Center(TBRC), Daejeon University) ;
  • Song, Hyang-Hee (Department of Health Skincare, Byuksung College) ;
  • Ji, Joong-Gu (Department of Oriental Health Care, Joongbu University) ;
  • Bak, Ji-Won (Traditional and Biomedical Research Center(TBRC), Daejeon University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 이병웅 (대전대학교 한의과대학 병리학교실) ;
  • 김선빈 (대전대학교 난치성면역질환의동서생명의학연구센터) ;
  • 송향희 (벽성대학 헬스스킨케어과) ;
  • 지중구 (중부대학교 한방건강관리학과) ;
  • 박지원 (대전대학교 난치성면역질환의동서생명의학연구센터) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2012.07.10
  • Accepted : 2012.08.20
  • Published : 2012.08.25
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Abstract

In order to evaluate the efficacy of SHT against atopic dermatitis (AD), various immune related cytokines as well as histological comparison were performed in animal models, and the results are described. Clinical skin index of the SHT treated group decreased significantly in weeks 11 and 13, compared to the control group. Also, CD4+ immune cell ratio in the dorsal skin was significantly decreased to 69%, and both epidermal and dermal skin thickness was decreased. Serum IL-4, IL-5, IL-6, IL-13, and TNF-${\alpha}$, which are all important markers of inflammation, were decreased to 64%, 44%, 87%, 48%, and 45%, respectively. The expression of histamine, a chemical transmitter increasingly released during the progression of inflammation, was significantly decreased to 47%. The production of IgE immunoglobulin was significantly decreased to 16% compared to the control group. In conclusion, SHT pacifies the activation of T cells, leading to suppression of both Th2 cytokine overexpression and infiltration of immune cells into skin. As a result, relative thinning of both epidermis and dermis were observed. With the results obtained from in vitro studies, the immune modulatory effect of SHT in AD animal models was experimentally demonstrated. This study should provide solid information to construct EBM and for clinical practice.

Keywords

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