International Journal of Industrial Entomology and Biomaterials

  • 제24권2호
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  • Pages.69-74
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  • 2012
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Studies on the High Temperature Induced Stress on the Biochemical Profile and Fecundity of Daba and Laria Ecoraces of Tropical Tasar Silkworm Antheraea mylitta Drury (Lepidoptera: Saturniidae)

  • Lokesh, G. (Silkworm Breeding & Genetics, Central Tasar Research & Training Institute, Central Silk Board) ;
  • Kar, P.K. (Silkworm Breeding & Genetics, Central Tasar Research & Training Institute, Central Silk Board) ;
  • Srivastava, A.K. (Silkworm Breeding & Genetics, Central Tasar Research & Training Institute, Central Silk Board) ;
  • Swaroopa, Saloni (Department of Biotechnology, Ranchi Women College, Ranchi University) ;
  • Sinha, M.K. (Silkworm Breeding & Genetics, Central Tasar Research & Training Institute, Central Silk Board)
  • 투고 : 2012.04.27
  • 심사 : 2012.06.28
  • 발행 : 2012.06.30
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초록

Tropical tasar silkworm Antheraea mylitta Drury experiences extreme temperature stress conditions during its life cycle particularly during diapauses and first crop. The present study witnessed the impact of high temperature on some biochemical profiles and egg production (fecundity) of semi-domesticated Daba and Shorea robusta (Sal) based wild ecorace Laria during seed cocoon (pupa) preservation. Cocoons of Daba and Laria were treated with high temperature at $40^{\circ}C$ for 10 days in a BOD incubator. The protein profile and carbohydrate content in the hemolymph and fat body and total haemocyte count (THC) in the hemolymph of pupa were investigated. Further, the fecundity and fertility of egg was assessed. Significant increase in the protein concentration was observed in the hemolymph with reduction in the fat body (p<0.05). The difference in protein concentration was highly significant between the semidomesticated Daba and wild ecorace Laria (p<0.05). High pupal mortality (20%) and reduced fecundity (10-15%) in Daba was noticed compared to wild Laria. Also an increased THC (>28000) was recorded in Laria. The study infers the potentials of wild ecoraces in sustaining the extreme temperature conditions and need of adopting suitable package of practices for the preservation of diapause seed cocoons during extreme summer conditions. There is possibility to introgression thermal stress resistant traits in the semi-domesticated races of tasar silkworm by resorting to conventional breeding plans with wild races and keeping the thermal stress induced response as markers.

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참고문헌

  1. Biram Saheb NM, Tribhuvan Singh, Saratchandra B (2009) Occurrence of unfertilized eggs in the mulberry silkworm, Bombyx mori L (Lepidoptera: Bombycidae). Int J Indust Entomol 18, 1-7.
  2. Blacklock BJ, Ryan RO (1994) hemolymph lipid transport. Insect Biochem Mol Biol 24, 855-873. https://doi.org/10.1016/0965-1748(94)90015-9
  3. Chaubey AK (2002) Postembryonic studies on hemocyte count in lemon-butterfly Papilio demoleus L. Ph. D. thesis, VBS Purvanchal University, Jaunpur, India.
  4. Chen Qiaofang, Gabriel GH (2004) Role of trehalose phosphate synthase and trehalose during hypoxia: from flies to mammals. J Exp Biol 207, 3125-3129. https://doi.org/10.1242/jeb.01133
  5. Duncan DB (1955) Multiple range and multiple F- tests. Biometrics 11, 1-42. https://doi.org/10.2307/3001478
  6. Forcella M, Berra E, Giacchini R, Parenti P (2007) Antioxidant defenses preserve membrane transport activity in Chironomus riparius larvar exposed to anoxia. Archv Insect Biochem Physiol. 65, 181-194. https://doi.org/10.1002/arch.20197
  7. Hansda G, RM Reddy, MK Sinha, Ojha NG, Vijaya Prakash NB (2008) EXsitu Stabilization and Utility prospects of Jata Ecorace of Tropical Tasar Silkworm Antheraea mylitta Drury. Intl J. Indus. Entomology 17(2), 169-172.
  8. Hazel JR (1995) Thermal adaptation on biological membranes: Is homeoviscous adaptation the explanation? Annu. Rev. Physiol. 57, 19-42. https://doi.org/10.1146/annurev.ph.57.030195.000315
  9. Joy O, Gopinathan KP (1995) Heat shock response in mulberry silkworm races with different thermo tolerances. Journal of Biosciences 20(4), 499-513. https://doi.org/10.1007/BF02703533
  10. Kapila ML, Chaudhuri A, Dubey OP, Chaudhuri CC, Sinha SS (1992) Studies on the preservation of seed cocoons of the tasar silkworm Antheraea mylitta D. during diapauses. Sericologia 32(4), 579-591.
  11. Kiran Kumar N, SM. Ismail, A. Dutta (1998) Differential uptake of storage protein by the fat body of Rice moth, Corcyra cephalonica during the larval pupal development. Entomon 23, 83-90.
  12. Kumar NS, HK. Basavaraja, PG. Joge, GV. Kalpana, N. Malreddy (2008) Heterosis studies on hybrids of cocoon color sex-limited breed of the silkworm, Bombyx mori L. under different environments of temperature. J. Entomol. Res. Soc 10, 1-12.
  13. Lowry OH, Rosebrough NF, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 267-275.
  14. Malik MA, Firdose Ahmad Malik (2009) Ontogenic Changes in hemolymph Biochemical Composition in the Silkworm, Bombyx mori L under Thermal Stress. Academic Journal of Entomology. 2(1) , 16-21.
  15. Manohar Reddy R, MK Sinha, BC Prasad (2010). Application of Parental Selection for Productivity Improvement in Tropical Tasar Silkworm Antheraea mylitta Drury-A Review. Journal of Entomology 7, 129-140. https://doi.org/10.3923/je.2010.129.140
  16. Nabizadeh Pezhman, TS Jagadeesh Kumar (2010) Thermal stress induced catalase activity level in selected bivoltine breeds of mulberry silkworm Bombyx mori L. Modern Applied Science 4(8), 88-95.
  17. Narain Raj, Rath SS, Negi BBS, Nagendra S, Kulshrestha V, Prasad BC, Sinha BRRP, Thangavelu K (2001) Integrated package for seed cocoon preservation and seed production in A.mylitta D. Indian Silk, 10, 15-17.
  18. Ojha NG, Saxena NN (1997) Tasar seed cocoon preservation, grainage technology and disease management. Base paper in current Technology Seminar on non-Mulberry Sericulture, Central Tasar Research & Training Institute, Ranchi.
  19. Pandey J P, Mishra P K, Kumar D, Singh BMK, Prasad BC (2010) Effect of temperature on hemocytic immune responses of tropical Tasar Silkworm, Antheraea mylitta D. Research Journal of Immunology 3(2), 169-177. https://doi.org/10.3923/rji.2010.169.177
  20. Shamitha G, Purushotham Rao A (2008) Effect of Gamma Irradiation on Tasar Silkmoth, Antheraea mylitta D. Asian J. Exp. Sci 22(3), 255-260.
  21. Singh Anitha, Ratnesh Kr Sharma, Bechan Sharma (2010) Low temperature induced alterations in certain biochemical constituents of 5th instar larvae of Philosamia ricini (Lepidoptera: Satunidae) Open Access Insect Physiology 2, 11-16.
  22. Singh Harjeet, Nair Suresh Kumar (2008) Selection of breeding resource natural for bivoltine silkworm Bombyx mori for breeding for high temperature tolerance. Ind J Seric 47, 20-28.
  23. Singh Harjeet, Nair Suresh Kumar (2010) On the Breeding of bivoltine double hybrid of Silkworm Bombyx mori (Lepidoptera: Bombycidae) Tolerant to High Temperature and Low humidity conditions of the tropics. African Journal of Basic & Applied Sciences 2(3-4), 71-80.
  24. Sinha BRRP (1998) Survey of genetic resources and study of the genetic divergence in Antheraea mylitta Drury. Ph.D. Thesis, Ranchi University, Ranchi, India.
  25. Sinha BRRP, Srivastava AK (2004) Path co-efficient analysis in yield contributing traits of twenty eight genotypes of Antheraea mylitta Drury. Pers Cytol Genet 11, 243- 249.
  26. Sinha USP, Sinha AK, Brahmachari BN, Sinha SS (1998) Seasonal variations in the carbohydrate content during different larval stages of Tasar silkworm, Antheraea mylitta Drury. Sericologia 38(2), 385-388.
  27. Srivartava AK, Naqvi AH, Sinha AK, Vishwakarma SR, Roy GC (2004) Genotype and environment interaction in Antheraea mylitta Drury and its implications. Pers Cytol Genet 11, 219-224.
  28. Srivartava AK, Naqvi AH, Sinha AK, Vishwakarma SR, Roy GC, Sinha BRRP (2002) Biotic diversity and genetic resource conservation of tropical tasar silkworm: National imperative, Advances in Indian Sericulture research (ed. Dandin S.B. and Gupta, V.P.), Central Sericultural Research and Training Institute, Central Silk Board, India. pp. 76-83.
  29. Srivastava AK, Kar PK, Naqvi AH, Sinha AK, Singh BMK, Sinha BRRP, Thangavelu K (2001) Biochemical variation in ecoraces of Antheraea mylitta Drury: A review. Perspect. Cytol. Genet. 10, 359-364.
  30. Suryanarayana N, Srivastava AK (2005) Monograph on Tropical Tasar Silkworm. Published by Central Tasar Research and Training Institute, CSB, India.
  31. Suryanarayana N, Kumar R, Gargi (2005) Monograph on Indian tropical tasar silkworm food plants. pp. 1-20, Published by Central Tasar Research and Training Institute, CSB, India.
  32. Tiwary RK, Shukla RS. (2000) Effect of certain stresses and 20-hydroxyecdysone injection on total hemocytes count in lemon-butterfly, Papilio demoleus L. Proc. Nat. Aca. Sci. India 70, 243-254.
  33. Willmer CW, Stone G, Johnston I. (2004). Environmental physiology of animals (Oxford: Blackwell Science).
  34. Wolfe RG, Donald LH, Michael ES (1998) A thermoprotective role for sorbitol in the silverleaf whitefly, Bemisia argentifolii. J Insect Physiol 44, 597-603. https://doi.org/10.1016/S0022-1910(98)00035-3
  35. Zhao Z. (1997) Progress in the research on mechanism of insect cold-hardiness. Entomol Sinica. 4(3), 265-276.

피인용 문헌

  1. Biochemical Performance and Quantitative Assessment of F1 Hybrid of Two Ecoraces of Tropical Tasar Silkworm Antheraea Mylitta Drury (Lepidoptera: Saturniidae) vol.26, pp.2, 2013, https://doi.org/10.7852/ijie.2013.26.2.67