Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

PCR-Based Detection of Toxoplasma gondii DNA in a Pet Rabbit

PCR 기법에 의한 애완용 토끼에서의 톡소플라즈마 유전자 검출

  • Kim, Dae-Hee (Department of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Park, Hyung-Jin (Department of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Sang-Hun (Department of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Song, Kun-Ho (Department of Veterinary Medicine, College of Veterinary Medicine, Chungnam National University)
  • 김대희 (충남대학교 수의과대학) ;
  • 박형진 (충남대학교 수의과대학) ;
  • 김상훈 (충남대학교 수의과대학) ;
  • 송근호 (충남대학교 수의과대학)
  • Accepted : 2012.08.21
  • Published : 2012.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

A 1-year-old female pet rabbit (Lion head, BW: 1.1 kg) was present to the animal hospital for vaccination of viral hemorrhagic disease. This rabbit was adopted in 4-month-old, and contacted with wild cats in outdoors before adoption. We examined feces for Toxoplasma gondii by PCR analysis. We detected the presence of the 497 base pair fragment as a positive result. This is the first detection of T. gondii DNA in a pet rabbit from Korea by PCR analysis.

1세의 암컷 애완용 토끼(라이온 헤드, 체중:1.1kg)가 바이러스성 출혈병에 대한 예방접종을 하기 위해 병원에 내원하였다. 평소 야생고양이와 자주 접촉한 사실을 문진상에서 확인하여 분변을 채취하여 PCR검사를 실시한 결과 톡소플라즈마 B1 유전자를 검출하였기에 이를 보고하고자 한다.

Keywords

References

  1. Almeria S, Calvete C, Pages A, Gauss C, Dubey JP. Factors affecting the seroprevalence of Toxoplasma gondii infection in wild rabbits (Oryctolagus cuniculus) from Spain. Vet Parasitol 2004; 123: 265-270. https://doi.org/10.1016/j.vetpar.2004.06.010
  2. Bergmann V, Heidrich R, Kiupel H. Acute toxoplasmosis outbreak in rabbit flocks. Angew Parasitol 1980; 21: 1-6 (in German).
  3. Beverley JKA, Beattie CP, Roseman C. Human Toxoplasma infection. J Hyg 1954; 52: 37-46. https://doi.org/10.1017/S0022172400027236
  4. Burg JL, Grover CM, Pouletty P, Boothroyd JC. Direct and sensitive detection of pathogenic protozoan Toxoplasma gondii by polymerase chain reaction. J Clin Microbiol 1989; 27: 1787-1792.
  5. Dubey JP, Beattie CP. Toxoplasmosis of animals and man. Boca Raton: CRC Press. 1988: 200.
  6. Dubey JP, Brown CA, Carpenter JL, Moore JJ 3rd. Fatal toxoplasmosis in domestic rabbits in the USA. Vet Parasitol 1992; 44: 305-309. https://doi.org/10.1016/0304-4017(92)90127-U
  7. Dubey JP, Frenkel JK. Experimental Toxoplasma infection in mice with strains producing oocysts. J Parasitol 1973; 59: 505-512. https://doi.org/10.2307/3278784
  8. Figueroa-Castillo JA, Duarte-Rosas V, Juárez-cevedo M, Luna-Pasten H, Correa D. Prevalence of Toxoplasma gondii antibodies in rabbits (Oryctolagus cuniculus) from Mexico. J Parasitol 2006; 92: 394-395. https://doi.org/10.1645/GE-663R.1
  9. Haziroglu R, Altintas K, Atasever A, Gulbahar M, Kul O, Tunca R. Pathological and immunohistochemical studies in rabbits experimentally infected with Toxoplasma gondii. Turk J Vet Anim Sci 2003; 27: 285-293.
  10. Ibragimova NM, Lopatinkiova VN. Rabbit as an important source of toxoplasmosis in humans. Trudy Med Inst Alma Ata 2003; 21: 505-507.
  11. Ishikawa T, Nishino H, Ohara M, Shimosato T, Naba K. The identification of a rabbit-transmitted cervical toxoplasmosis mimicking malignant lymphoma. Am J Clin Pathol 1990; 94: 107-110.
  12. Janitschke K. Animals as a source of Toxoplasma infection in man. Dtsch Med Wschr 1971; 96: 78-83. https://doi.org/10.1055/s-0028-1108207
  13. Jones CD, Okhravi N, Adamson P, Tasker S, Lightman S. Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor. Invest Ophthalmol Vis Sci 2000; 41: 634-644.
  14. Lainson R. Toxoplasmosis in England. I. The rabbit (Oryctolagus cuniculus) as a host of Toxoplasma gondii. Ann Trop Med Parasitol 1955; 49: 384-396.
  15. Leland MM, Hubbard GB, Dubey JP. Clinical toxoplasmosis in domestic rabbits. Lab Anim Sci 1992; 42: 318-319.
  16. Levaditi C, Schsoen R, Sanchsis-Bayarhi V. Spontaneous encephalitis in rabbits evoked by Toxoplasma gondii. Cr Soc Biol 1927; 97: 1692-1693.
  17. Lindsay DS, Dubey JP, Butler JM, Blagburn BL. Mechanical transmission of Toxoplasma gondii oocysts by dogs. Vet Parasitol 1997; 73: 27-33. https://doi.org/10.1016/S0304-4017(97)00048-4
  18. Siim JC, Biering-Sorensen U. Toxoplasmosis in domestic animals. Adv Vet Sci 1963; 19: 350-360.
  19. Sima O, Rasin K. Toxoplasma gondii Nicolle et Manceaux 1909-antibodies in domestic rabbits. Vet Med 1973; 18: 633-640
  20. Splendore A. Novel protozoan parasite of rabbits. Rev Soc Sci Sao Paulo 1966; 3: 109-112.
  21. Sroka J. Seroepidemiology of toxoplasmosis in the Lublin region. Ann Agric Environ Med 2001; 8: 25-31.
  22. Tenter AM, Heckeroth AR, Weiss LM. Toxoplasma gondii: from animals to humans. Int J Parasitol 2000; 30: 1217-1258. https://doi.org/10.1016/S0020-7519(00)00124-7