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Comparison of the bone healing capacity of autogenous bone, demineralized freeze dried bone allograft, and collagen sponge in repairing rabbit cranial defects

  • Hur, Jung-Woo (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Yoon, Suk-Ja (Department of Oral & Maxillofacial Radiology, School of Dentistry, Chonnam National University) ;
  • Ryu, Sun-Youl (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University)
  • Received : 2012.04.13
  • Accepted : 2012.07.16
  • Published : 2012.08.30

Abstract

Objectives: This study sought to evaluate the efficacy of collagen graft materials, as compared to other graft materials, for use in healing calvarial defects in rabbits. Materials and Methods: Ten mm diameter calvarial defects were made in ten rabbits. The rabbits were then divided into 4 groups: control, autogenous bone graft, SureOss graft, and Teruplug graft. Bone regeneration was evaluated using histological and radiographic methods. Results: Based on visual examination, no distinct healing profile was observed. At 4 weeks after treatment, histological analysis showed there was no bone regeneration in the control group; however, at 8 weeks after treatment, new bone formation was observed around the margin of the defective sites. In the autogenous bone graft group, new bone formation was observed at 4 weeks after treatment and mature bone was detected around the grafted bone after 8 weeks. In the SureOss graft group, at 4 weeks after treatment, acute inflammatory and multinuclear cells were noted around the grafted materials; at 8 weeks after treatment, a decrease in graft materials coupled with new bone formation were observed at the defective sites. In the Teruplug graft group, new bone formation was detected surrounding the bone margin and without signs of inflammation. There were statistically significant differences observed between the graft and control group in terms of bone density as evidenced by radiographic analysis using computed tomography (P<0.05), particularly for the autogenous bone graft group (P<0.001). Conclusion: These results suggested that autogenous bone, SureOss and Teruplug have the ability to induce bone regeneration as compared to an untreated control group. The osteogenic potential of Teruplug was observed to be lower than that of autogenous bone, but similar to that of SureOss.

Keywords

References

  1. Lee EJ, Chung HJ. Histologic study on healing after implantation of several bone substitutes in rat calvarial defects. J Korean Acad Periodontol 1998;28:87-102.
  2. Misch CE, Dietsh F. Bone-grafting materials in implant dentistry. Implant Dent 1993;2:158-67.
  3. Lane JM. Bone graft substitutes. West J Med 1995;163:565-6.
  4. Rummelhart JM, Mellonig JT, Gray JL, Towle HJ. A comparison of freeze-dried bone allograft and demineralized freeze-dried bone allograft in human periodontal osseous defects. J Periodontol 1989;60:655-63.
  5. Kim SH, Kim CK, Chai JK, Cho KS. Clinical study on therapeutic effects of decalcified freeze dried bone allograft in intrabony defects. J Korean Acad Periodontol 1994;24:618-32.
  6. Hislop WS, Finlay PM, Moos KF. A preliminary study into the uses of anorganic bone in oral and maxillofacial surgery. Br J Oral Maxillofac Surg 1993;31:149-53.
  7. Schepers EJ, Ducheyne P, Barbier L, Schepers S. Bioactive glass particles of narrow size range: a new material for the repair of bone defects. Implant Dent 1993;2:151-6.
  8. Urist MR. Bone: formation by augmentation. Science 1965;150: 893-9.
  9. Libin BM, Ward HL, Fishman L. Decalcified, lyophilized bone allografts for use in human periodontal defects. J Periodontol 1975; 46:51-6.
  10. Acil Y, Springer IN, Broek V, Terheyden H, Jepsen S. Effects of bone morphogenetic protein-7 stimulation on osteoblasts cultured on different biomaterials. J Cell Biochem 2002;86:90-8.
  11. Wikesjo UM, Sorensen RG, Kinoshita A, Wozney JM. RhBMP-2/alphaBSM induces significant vertical alveolar ridge augmentation and dental implant osseointegration. Clin Implant Dent Relat Res 2002;4:174-82.
  12. Bowers GM, Chadroff B, Carnevale R, Mellonig J, Corio R, Emerson J, et al. Histologic evaluation of new attachment apparatus formation in humans. Part III. J Periodontol 1989;60:683-93.
  13. Kim JH, Kim CH, Kim KW. Bone healing capacity of the collagen bone filler (TERUPLUG(R)) and rhBMP-2 in the rabbit cranium defect. J Korean Assoc Oral Maxillofac Surg 2008;34:119-30.
  14. Lekovic V, Kenney EB, Weinlaender M, Han T, Klokkevold P, Nedic M, et al. A bone regenerative approach to alveolar ridge maintenance following tooth extraction. Report of 10 cases. J Periodontol 1997;68:563-70.
  15. Lang N, Becker W, Karring T. Alveolar bone formation. In: Lindhe J. Textbook of clinical periodontology and implant dentistry. 3rd ed. Copenhagen: Munksgaard; 1998:906-32.
  16. Irinakis T, Tabesh M. Preserving the socket dimensions with bone grafting in single sites; an esthetic surgical approach when planning delayed implant placement. J Oral Implantol 2007;33:156-63.
  17. Frame JW. Hydroxyapatite as a biomaterial for alveolar ridge augmentation. Int J Oral Maxillofac Surg 1987;16:642-55.
  18. Pinholt EM, Bang G, Haanaes HR. Alveolar ridge augmentation in rats by combined hydroxylapatite and osteoinductive material. Scand J Dent Res 1991;99:64-74.
  19. Sepe WW, Bowers GM, Lawrence JJ, Friedlaender GE, Koch RW. Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects--part II. J Periodontol 1978;49:9-14.
  20. Koole R, Bosker H, van der Dussen FN. Late secondary autogenous bone grafting in cleft patients comparing mandibular (ectomesenchymal) and iliac crest (mesenchymal) grafts. J Craniomaxillofac Surg 1989;17 Suppl 1:28-30.
  21. Matzenbacher SA, Mailhot JM, McPherson JC 3rd, Cuenin MF, Hokett SD, Sharawy M, et al. In vivo effectiveness of a glycerol-compounded demineralized freeze-dried bone xenograft in the rat calvarium. J Periodontol 2003;74:1641-6.
  22. Senn N. On the healing of aseptic bone cavities by implantation of antiseptic decalcified bone. Ann Surg 1889;10:352-68.
  23. Kalish BP, Schuster GS, Peacock ME, Cuenin MF, Swiec GD, Potter BJ, et al. Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium. J Oral Implantol 2008;34:83-9.
  24. Makoto H, Nobuyuki M, Shinjiro A, Susumu O, Kei W, Tomomichi O, et al. Efficacy of tooth extraction wound protection made of atelocollagen sponge (TRE-641): a pilot study in dogs. J Hard Tissue Biology 2009;18:89-94.
  25. Koide M, Osaki K, Konishi J, Oyamada K, Katakura T, Takahashi A, et al. A new type of biomaterial for artificial skin: dehydrothermally cross-linked composites of fibrillar and denatured collagens. J Biomed Mater Res 1993;27:79-87.
  26. Konishi J. Composition of collagen materials. Jpn J Artif Organs 1989;18:155-8.