Asian Pacific Journal of Cancer Prevention

  • 제13권5호
  • /
  • Pages.2115-2120
  • /
  • 2012
  • /
  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
권호 표지
DOI QR코드

DOI QR Code

Hypoxia-Inducible Factor 1 Promoter-Induced JAB1 Overexpression Enhances Chemotherapeutic Sensitivity of Lung Cancer Cell Line A549 in an Anoxic Environment

  • Hu, Ming-Dong (Institute of Respiration, the Second Affiliated Hospital, the Third Military Medical University) ;
  • Xu, Jian-Cheng (Institute of Respiration, the Second Affiliated Hospital, the Third Military Medical University) ;
  • Fan, Ye (Institute of Respiration, the Second Affiliated Hospital, the Third Military Medical University) ;
  • Xie, Qi-Chao (Department of Tumors, the Second Affiliated Hospital, the Third Military Medical University) ;
  • Li, Qi (Institute of Respiration, the Second Affiliated Hospital, the Third Military Medical University) ;
  • Zhou, Chang-Xi (The General Hospital of PLA) ;
  • Mao, Mei (Wuhan General Hospital of Guangzhou Military Regions) ;
  • Yang, Yu (Institute of Respiration, the Second Affiliated Hospital, the Third Military Medical University)
  • 발행 : 2012.05.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The presence of lung cancer cells in anoxic zones is a key cause od chemotherapeutic resistance. Thus, it is necessary to enhance the sensitivity of such lung cancer cells. However, loss of efficient gene therapeutic targeting and inefficient objective gene expression in the anoxic zone in lung cancer are dilemmas. In the present study, a eukaryotic expression plasmid pUC57-HRE-JAB1 driven by a hypoxia response elements promoter was constructed and introduced into lung cancer cell line A549. The cells were then exposed to a chemotherapeutic drug cis-diamminedichloroplatinum (C-DDP). qRT-PCR and western blotting were used to determine the mRNA and protein level and flow cytometry to examine the cell cycle and apoptosis of A549 transfected pUC57-HRE-JAB1. The results showed that JAB1 gene in the A549 was overexpressed after the transfection, cell proliferation being arrested in G1 phase and the apoptosis ratio significantly increased. Importantly, introduction of pUC57-HRE-JAB1 significantly increased the chemotherapeutic sensitivity of A549 in an anoxic environment. In conclusion, JAB1 overexpression might provide a novel strategy to overcome chemotherapeutic resistance in lung cancer.

키워드

참고문헌

  1. Bando H, Toi M, Kitada K, Koike M (2003). Genes commonly upregulated by hypoxia in human breast cancer cells MCF-7 and MDA-MB-231. Biomed Pharmacother, 57, 333-40. https://doi.org/10.1016/S0753-3322(03)00098-2
  2. Cheah PL, Looi LM, Teoh KH, Mun KS, Nazarina AR (2012). p16INK4a is a useful marker of human papillomavirus integration allowing risk stratification for cervical malignancies. Asian Pac J Cancer Prev, 13, 469-72. https://doi.org/10.7314/APJCP.2012.13.2.469
  3. Claret FX, Hibi M, Dhut S, Toda T, Karin M (1996). A new group of conserved coactivators that increase the specificity of AP-1 transcription factors. Nature, 383, 453-7. https://doi.org/10.1038/383453a0
  4. Dachs GU, Patterson AV, Firth JD, et al (1997). Targeting gene expression to hypoxic tumor cells. Nat Med, 3, 515-20. https://doi.org/10.1038/nm0597-515
  5. Hallstrom TC, Nevins JR (2006). JAB1 is a specificity factor for E2F1-induced apoptosis. Genes Dev, 20, 613-23. https://doi.org/10.1101/gad.1345006
  6. Harrison L, Blackwell K (2004). Hypoxia and anemia: factors in decreased sensitivity to radiation therapy and chemotherapy? Oncologist, 5, 31-40.
  7. Hayashi M, Sakata M, Takeda T, et al (2004). Induction of glucose transporter 1 expression through hypoxia-inducible factor $1{\alpha}$ under hypoxic conditions in trophoblast-derived cells. J Endocrinol, 183, 145-54. https://doi.org/10.1677/joe.1.05599
  8. Kouvaraki MA, Korapati AL, Rassidakis GZ, et al (2006). Potential role of Jun activation domain-binding protein 1 as a negative regulator of P27kip1 in pancreatic adenocarcinoma. Cancer Res, 66, 8581-9. https://doi.org/10.1158/0008-5472.CAN-06-0975
  9. Kouvaraki MA, Rassidakis GZ, Tian L, et al (2003). Jun activation domain-binding protein 1 expression in breast cancer inversely correlates with the cell cycle inhibitor p27kip1. Cancer Res, 63, 2977-81.
  10. Lara PC, Lloret M, Clavo B, et al (2009). Severe hypoxia induces chemo-resistance in clinical cervical tumors through MVP over-expression. Radiat Oncol, 4, 29. https://doi.org/10.1186/1748-717X-4-29
  11. Lee SH, Kim J, Kim WH, Lee YM (2009). Hypoxic silencing of tumor suppressor RUNX3 by histone modification in gastric cancer cells. Oncogene, 28, 184-94. https://doi.org/10.1038/onc.2008.377
  12. Liao HY, Wang GP, Gu LJ, et al (2012). HIF-$1\alpha$ siRNA and cisplatin in combination suppresstumor growth in a nude mice model of esophageal squamous cell carcinoma. Asian Pac J Cancer Prev, 13, 469-72. https://doi.org/10.7314/APJCP.2012.13.2.469
  13. Liu X, Pan Z, Zhang L, et al (2008). JAB1 accelerates mitochondrial apoptosis by interaction with proapoptotic BclGs. Cell Signal, 20, 230-40. https://doi.org/10.1016/j.cellsig.2007.10.012
  14. Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25, 402-8. https://doi.org/10.1006/meth.2001.1262
  15. Maher JC, Wangpaichitr M, Savaraj N, Kurtoglu M, Lampidis TJ (2007). Hypoxia-inducible factor-1 confers resistance to the glycolytic inhibitor 2-deoxy-D-glucose. Mol Cancer Ther, 6, 732-41.
  16. Osoegawa A, Yoshino I, Kometani T, et al (2006). Overexpression of Jun activation domain-binding protein 1 in nonsmall cell lung cancer and its significance in p27 expression and clinical features. Cancer, 107, 154-61. https://doi.org/10.1002/cncr.21961
  17. Pommier Y, Sordet O, Antony S, Hayward RL, Kohn KW (2004). Apoptosis defects and chemotherapy resistance: molecular interaction maps and networks. Oncogene, 23, 2934-49. https://doi.org/10.1038/sj.onc.1207515
  18. Post DE, Van Meir EG (2001). Generation of bidirectional hypoxia/HIF-responsive expression vectors to target gene expression to hypoxic cells. Gene Ther, 8, 1801-7. https://doi.org/10.1038/sj.gt.3301605
  19. Ruan H, Su H, Hu L, et al (2001). A hypoxia-regulated adenoassociated virus vector for cancer-specific gene therapy. Neoplasia, 3, 255-63. https://doi.org/10.1038/sj.neo.7900157
  20. Semenza GL (1999). Regulation of mammalian O2 homeostasis by hypoxia-inducible factor 1. Annu Rev Cell Dev Biol, 15, 551-78. https://doi.org/10.1146/annurev.cellbio.15.1.551
  21. Semenza GL (2000). HIF-1: mediator of physiological and pathophysiological responses to hypoxia. J Appl Physiol, 88, 1474-80. https://doi.org/10.1152/jappl.2000.88.4.1474
  22. Semenza GL, Jiang BH, Leung SW, et al (1996). Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. J Biol Chem, 271, 32529-37. https://doi.org/10.1074/jbc.271.51.32529
  23. Shintani S, Li C, Mihara M, Hino S, Nakashiro K, Hamakawa H (2003). Skp2 and Jab1 expression are associated with inverse expression of p27kip1 and poor prognosis in oral squamous cell carcinomas. Oncology, 65, 355-62. https://doi.org/10.1159/000074649
  24. Tamura N, Dong Y, Sui L, et al (2001). Cyclin-dependent kinase inhibitor p27 is related to cell proliferation and prognosis in laryngeal squamous cell carcinomas. J Laryngol Otol, 115, 400-6.
  25. Toh CK (2009). The changing epidemiology of lung cancer. Methods Mol Biol, 472, 397-411. https://doi.org/10.1007/978-1-60327-492-0_19
  26. Tomoda K, Kubota Y, Arata Y, et al (2002). The cytoplasmic shuttling and subsequent degradation of p27kip1 mediated by JAB1/CSN5 and the COP9 signalosome complex. Biol Chem, 277, 2302-10. https://doi.org/10.1074/jbc.M104431200
  27. Tomoda K, Yoneda-Kato N, Fukumoto A, Yamanaka S, Kato JY (2004). Multiple functions of Jab1 are required for early embryonic development and growth potential in mice. J Biol Chem, 279, 43013-8. https://doi.org/10.1074/jbc.M406559200
  28. Wang GL, Semenza GL (1993). General involvement of hypoxiainducible factor 1 in transcriptional response to hypoxia. Proc Natl Acad Sci U S A, 90, 4304-8. https://doi.org/10.1073/pnas.90.9.4304
  29. Wang YC, Fei M, Cheng C, et al (2007). Jun Activation Domain- Binding Protein 1 negatively regulate p27 (kip1) in Non- Hodgkinps Lymphomas. Cancer Biol Ther, 7, 460-7.
  30. Xie QC, Hu YD, Wang LL, et al (2005). The co-transfection of p16 (INK4a) and p14 (ARF) genes into human lung cancer cell line A549 and the effects on cell growth and chemosensitivity. Colloids Surf B Biointerfaces, 46, 188-96. https://doi.org/10.1016/j.colsurfb.2005.10.006
  31. Yang QC, Zeng BF, Shi ZM, et al (2006). Inhibition of hypoxiainduced angiogenesis by trichostatin A via suppression of HIF-1a activity in human osteosarcoma. J Exp Clin Cancer Res, 25, 593-9.
  32. Yasuda H (2008). Solid tumor physiology and hypoxia-induced chemo/radio-resistance: novel strategy for cancer therapy: nitric oxide donor as a therapeutic enhancer. Nitric Oxide, 19, 205-16. https://doi.org/10.1016/j.niox.2008.04.026

피인용 문헌

  1. Effects of Ribosomal Protein L39-L on the Drug Resistance Mechanisms of Lung Cancer A549 Cells vol.15, pp.7, 2014, https://doi.org/10.7314/APJCP.2014.15.7.3093
  2. Knockdown of Med19 Suppresses Proliferation and Enhances Chemo-sensitivity to Cisplatin in Non-small Cell Lung Cancer Cells vol.16, pp.3, 2015, https://doi.org/10.7314/APJCP.2015.16.3.875
  3. Upregulation of Id3 inhibits cell proliferation and induces apoptosis in A549/DDP human lung cancer cells in vitro vol.14, pp.1, 2016, https://doi.org/10.3892/mmr.2016.5221
  4. In silico identification of potential key regulatory factors in smoking-induced lung cancer vol.10, pp.1, 2017, https://doi.org/10.1186/s12920-017-0284-z
  5. miR-21 modulates paclitaxel sensitivity and hypoxia-inducible factor-1α expression in human ovarian cancer cells vol.6, pp.3, 2013, https://doi.org/10.3892/ol.2013.1432