Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

Genetic Relationship of Mono-cotyledonous Model Plant by Ionizing Irradiation

단자엽 모델 식물의 방사선원 별 처리에 따른 유전적 다형성 분석

  • Song, Mira (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sun-Hee (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jang, Duk-Soo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ha, Bo-Keun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong Sub (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 송미라 (한국원자력연구원 첨단방사선연구소) ;
  • 김선희 (한국원자력연구원 첨단방사선연구소) ;
  • 장덕수 (한국원자력연구원 첨단방사선연구소) ;
  • 강시용 (한국원자력연구원 첨단방사선연구소) ;
  • 김진백 (한국원자력연구원 첨단방사선연구소) ;
  • 김상훈 (한국원자력연구원 첨단방사선연구소) ;
  • 하보근 (한국원자력연구원 첨단방사선연구소) ;
  • 김동섭 (한국원자력연구원 첨단방사선연구소)
  • Received : 2012.01.13
  • Accepted : 2012.02.20
  • Published : 2012.03.31

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Abstract

In this study, we investigated the genetic variation in the general of monocot model plant (rice) in response to various ionizing irradiations including gamma-ray, ion beam and cosmic-ray. The non-irradiated and three irradiated (200 Gy of gamma-ray and 40 Gy of ion beam and cosmic-ray) plants were analyzed by AFLP technique using capillary electrophoresis with ABI3130xl genetic analyzer. The 29 primer combinations tested produced polymorphism results showing a total of 2,238 bands with fragments sizes ranged from 30 bp to 600 bp. The number of polymorphism generated by each primer combinations was varied significantly, ranging from 2 (M-CAC/E-ACG) to 158 (M-CAT/E-AGG) with an average of 77 bands. Polymorphic peaks were detected as 1,269 with an average of 44 per primer combinations. By UPGMA (Unweighted Pair Group Method using Arithmetic clustering) analysis method, the clusters were divided into non-irradiated sample and three irradiated samples at a similarity coefficient of 0.41 and three irradiation samples was subdivided into cosmic-ray and two irradiation samples (200 Gy of gamma-ray and 40 Gy of ion beam) at similarity coefficient of 0.48. Similarity coefficient values ranged from 0.41 to 0.55.

Keywords

Acknowledgement

Supported by : 한국원자력연구원