Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

In Vitro Anti-inflammatory Activities of Herbal Extracts with Eucommia ulimoides and Ulmus davidiana

두충과 느릅 혼합추출물에 의한 염증 억제 효과

  • Gwon, Hui-Jeong (Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Shin, Young Min (Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jung, Sung-In (Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Jong-Seok (Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Nho, Young-Chang (Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lim, Youn-Mook (Research Division for Industry & Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 권희정 (한국원자력연구원 첨단방사선연구소 공업환경연구부) ;
  • 신영민 (한국원자력연구원 첨단방사선연구소 공업환경연구부) ;
  • 정성린 (한국원자력연구원 첨단방사선연구소 공업환경연구부) ;
  • 박종석 (한국원자력연구원 첨단방사선연구소 공업환경연구부) ;
  • 노영창 (한국원자력연구원 첨단방사선연구소 공업환경연구부) ;
  • 임윤묵 (한국원자력연구원 첨단방사선연구소 공업환경연구부)
  • Received : 2011.12.14
  • Accepted : 2012.01.30
  • Published : 2012.03.31

⟨ Previous Next ⟩

Abstract

This study confirmed the cytotoxicity and anti-inflammatory activities of natural herbal extracts (HE) including Eucommia ulimoides and Ulmus davidiana against human mast cell (HMC-1). HE was extracted with distilled water (at $75^{\circ}C$) and then freeze-dried for 5 days. Finally, the HE was sterilized by gamma radiation with $^{60}Co$ ${\gamma}$ source at room temperature. Cytotoxicity of the HE against HMC-1 cell was measured using cell counting kit-8 (CCK-8) assay. In addition, inflammatory cytokines such as $TNF-{\alpha}$, IL-6 and IL-8 were evaluated by ELISA kit on the HMC-1 cells with calcimycin A23187 and phorbol 12-myristate 13-acetate (PMA). The results showed that HE had no toxicity and reduced $TNF-{\alpha}$, IL-6, IL-8 response on HMC-1 cells.

Keywords

Acknowledgement

Supported by : 한국과학재단