Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Trans-unsaturated Fatty Acid on Serum Lipid Levels in Mice

트랜스형 불포화 지방산이 mice 혈중 지질농도에 미치는 영향

  • Yu, Sun-Nyoung (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Ahn, Jeong-Bin (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology) ;
  • Park, Eun-Young (Department of Chemical Engineering, College of Engineering, Pukyong University) ;
  • Lee, Sun-Jung (Department of Health Care Management, Catholic University of Pusan) ;
  • Tak, Min-Gi (Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sunkyunkwan University) ;
  • Kim, Kwang-Youn (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Kim, Sang-Hun (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Kim, Ki-Dae (DongNae Girls' High School) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine)
  • 유선녕 (부산대학교 의학전문대학원 미생물학 및 면역학교실) ;
  • 안정빈 (울산과학기술대학교 나노생명화학공학부) ;
  • 박은영 (부경대학교 공과대학 화학공학과) ;
  • 이선정 (부산가톨릭대학교 보건과학대학 병원경영학과) ;
  • 탁민지 (성균관대학교 생명공학대학 유전공학과) ;
  • 김광연 (부산대학교 의학전문대학원 미생물학 및 면역학교실) ;
  • 김상헌 (부산대학교 의학전문대학원 미생물학 및 면역학교실) ;
  • 김기대 (동래여자고등학교) ;
  • 안순철 (부산대학교 의학전문대학원 미생물학 및 면역학교실)
  • Received : 2012.06.26
  • Accepted : 2012.08.24
  • Published : 2012.08.30
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Abstract

The purpose of this study was to investigate the effects of trans-unsaturated fatty acid (TFA), saturated fatty acid (SFA) and general unsaturated fatty acid (USFA) on serum lipid levels in ICR mice. Male ICR 8-week-old mice were divided into six groups; the control group (C) fed with normal diet, the TFA-1 group fed with 10% trans-unsaturated fatty acid, the TFA-2 group fed with 30% trans-unsaturated fatty acid, the TFA-3 group fed with 50% trans-unsaturated fatty acid, the SFA group fed with 50% saturated fatty acid, and USFA group fed with 50% general unsaturated fatty acid. The serum total cholesterol of TFA-3 and SFA was higher than those of other fat groups and C. The serum triglyceride (TG) of TFA-3 and SFA showed the highest levels among all of diet groups. Also the serum HDL cholesterol levels of TFA-3 showed the lowest. LDL cholesterol and atherogenic indices (AI) were remarkedly increased in TFA-3 and SFA, as compared with other fat fed groups and C. Taken together with results, the TFA-3 group showed serum lipid levels similar to the SFA and different from the USFA. These results suggest that intake of high level of trans-unsaturated fatty acid increased serum triglyceride, LDL cholesterol and atherogenic indices, which may affect risk on serum lipid level for lipid metabolism syndrome.

본 연구에서는 각기 다른 형태의 지방산이 ICR mice의 혈중 지질 농도에 미치는 영향을 평가하고자 하였다. 이를 위하여 8주령 수컷 ICR mice를 일반 식이섭취군(C), 10%의 트랜스 불포화 지방산 섭취군(TFA-1), 30% 트랜스 불포화 지방산 섭취군(TFA-2), 50% 트랜스 불포화 지방산 섭취군(TFA-3), 50% 포화 지방산 섭취군(SFA), 불포화 지방산 섭취군(USFA) 으로 나누어 식이 하였다. 혈중 총 콜레스테롤의 수치는 TFA-3 군과 SFA 군의 지질함량이 다른 군에 비하여 높았으며, 중성지방지수 역시 높게 나타났다. 총 콜레스테롤과 중성지방수치를 이용하여 나타낸 LDL 콜레스테롤의 경우에도 50%의 트랜스 지방산을 섭취한 군에서 포화 지방산을 섭취한 군과 유사한 결과가 나타났다. 이에 반해, 10%, 30%의 트랜스 지방산을 섭취한 군에서는 일반식이 섭취군과 비슷한 결과가 나타났다. 이는 많은 양의 트랜스 불포화지방산을 짧은 기간 동안 섭취하더라도 포화지방산을 섭취한 것과 유사한 효과를 나타낸다는 것을 의미한다.

Keywords

References

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