생명과학회지 (Journal of Life Science)

  • 제22권8호
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  • Pages.1071-1076
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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사방오리나무 추출물의 항우식 및 항구취 효과

Antimicrobial and Anti-halitosis Effects of Alnus firma Extracts

  • 최혜정 (창원대학교 생명공학 협동과정) ;
  • 허남숙 (마산대학교 치위생학과) ;
  • 최영환 (부산대학교 원예생명자원학과) ;
  • 이영근 (부산대학교 식품공학과) ;
  • 정영기 (동아대학교 생명공학과) ;
  • 주우홍 (창원대학교 생명공학 협동과정)
  • Choi, Hye-Jung (Department of Biology and Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Heo, Nam-Suk (Department of Dental Hygiene, Masan University) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, Pusan National University) ;
  • Lee, Young-Geun (Department of Food Science and Technology, Pusan National University) ;
  • Jeong, Young-Kee (Department of Biotechnology, Dong-A University) ;
  • Joo, Woo-Hong (Department of Biology and Interdisciplinary Program in Biotechnology, Changwon National University)
  • 투고 : 2012.06.09
  • 심사 : 2012.07.17
  • 발행 : 2012.08.30
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초록

사방오리나무의 분획물과 분리화합물인 gallic acid의 항우식 작용과 항구취 활성을 조사하기 위해 구강병원균에 대한 항균활성, 부착능 그리고 산 생성을 조사하였다. 그리고 휘발성 황화합물의 측정은 oral chroma를 이용하여 구취억제 효과를 측정하였다. DCM 분획물은 넓은 항균스펙트럼을 가졌으며, EA 분획물은 Streptococcus mutans와 Porphyromons gingivalis에 대해 상대적으로 높은 항균활성을 가진 것으로 나타났다. 게다가 GA와 DCM 분획물은 각각 S. mutans와 S. salivarius에 대해 산 생성과 부착을 효과적으로 억제하였다. 사방오리나무의 2% MeOH 추출물은 구취유발 화합물인 황화수소, 메틸머캅탄, 황화디메틸을 억제하는 효과가 뛰어났으며(p<0.05), 2% GA 또한 황화수소를 억제시키는 효과가 있음이 확인되었다(p<0.05). 본 연구의 결과, 사방오리나무의 활성 분획물과 GA는 항균효능, 산생성 억제능, 부착 억제능 및 구취 억제효능을 가짐으로써 구강병원균에 대한 천연항균제제로써의 사용 가능성이 충분히 있는 것으로 판단된다.

To investigate the antimicrobial and anti-halitosis effects of Alnus firma extracts and gallic acid (GA) isolated from A. firma, we measured their antimicrobial activities against oral pathogens and their inhibitory effects on the cell adhesiveness and acid production of oral pathogens. In addition, the levels of volatile sulfur compounds were determined by using oral chroma. The dichloromethane (DCM) fraction has broad antimicrobial activity, and the ethylacetate (EA) fraction showed a relatively high level of antimicrobial activity against Streptococcus mutans and Porphyromons gingivalis. Especially, the GA and DCM fractions had significant inhibitory effects on the attachment and acid production of S. mutans and Streptococcus salivarius, respectively. The 2% MeOH extract of A. firma showed a significant inhibitory effect on the production of volatile oral compounds, such as hydrogen sulfide, methyl mercaptan, and dimethyl sulfide, which can cause bad breath and halitosis. Two percent GA also had a significant inhibitory effect on the production of hydrogen sulfide. Our study showed that the active fractions and GA of A. firma could be suitable resources for development as a natural antibiotic agent for the treatment of infectious oral diseases.

키워드

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