YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antihypertensive Effects of Novel Isoflavone-Free Black Soy Peptide Mixture as HO-1 Inducer

Heme 산화효소 발현 유도체로서 Isoflavone-Free 검은콩 펩타이드의 항고혈압 활성

  • Shin, Mi-Kyung (Department of Pharmacology and Medicinal Toxicology Research Center, Center for Advanced Medical Education, Inha University College of Medicne by BK-21 Project, Inha University) ;
  • Kwon, Yong-Hyun (Department of Pharmacology and Medicinal Toxicology Research Center, Center for Advanced Medical Education, Inha University College of Medicne by BK-21 Project, Inha University) ;
  • Ahn, Chang-Won (R&BD Center, NongShim Co., Ltd.) ;
  • Shin, Dong-Seok (R&BD Center, NongShim Co., Ltd.) ;
  • Park, Soo-Hyun (R&BD Center, NongShim Co., Ltd.) ;
  • Choi, Bo-Hwa (Department of Pharmacology and Medicinal Toxicology Research Center, Center for Advanced Medical Education, Inha University College of Medicne by BK-21 Project, Inha University) ;
  • Hong, Soon-Sun (Department of Biomedical Sciences and Clinical Research Center, Inha University) ;
  • Kang, Ju-Hee (Department of Pharmacology and Medicinal Toxicology Research Center, Center for Advanced Medical Education, Inha University College of Medicne by BK-21 Project, Inha University) ;
  • Park, Chang-Shin (Department of Pharmacology and Medicinal Toxicology Research Center, Center for Advanced Medical Education, Inha University College of Medicne by BK-21 Project, Inha University)
  • 신미경 (인하대학교 의과대학 의학전문대학원 약리학교실 의약물독성연구소) ;
  • 권용현 (인하대학교 의과대학 의학전문대학원 약리학교실 의약물독성연구소) ;
  • 안창원 ((주)농심 R&BD 센터) ;
  • 신동석 ((주)농심 R&BD 센터) ;
  • 박수현 ((주)농심 R&BD 센터) ;
  • 최보화 (인하대학교 의과대학 의학전문대학원 약리학교실 의약물독성연구소) ;
  • 홍순선 (인하대학교 특성화교실 임상시험센터) ;
  • 강주희 (인하대학교 의과대학 의학전문대학원 약리학교실 의약물독성연구소) ;
  • 박창신 (인하대학교 의과대학 의학전문대학원 약리학교실 의약물독성연구소)
  • Received : 2012.05.18
  • Accepted : 2012.06.15
  • Published : 2012.06.30
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Abstract

We previously reported that the novel isoflavone-free peptide mixture (black soybean peptide, BSP) had several beneficial effects like antiobesity and hypotriglyceridemic effect. However, there are no reports for BSP on anti-hypertensive activity. BSP induced heme oxygenase-1 (HO-1) in HUVECs, thus investigated the HO-1-induced activity in HUVECs and the anti-hypertensive effects in SHR animal model. BSP significantly induced HO-1 expression both at transcriptional and protein levels in a time- and dose-dependent manner as measured by RT-PCR and Western blot analysis, respectively. These inductions were abolished by pretreatment of N-acetyl-cystein (NAC, 1~10 mM), but not by employing Tempol, a superoxide dismutase (SOD) mimetic (1~5 mM). As expected, enzymatic activity (~2 fold) determined by bilirubin formation assay and cGMP concentration (~6 fold) were significantly increased in BSP-treated cells. Based on the numerous evidences on the beneficial effects of HO-1 and our results, we investigated in vivo effects of BSP on the antihypertensive activity. The administration of BSP (1% in drinking water) significantly decreased mean blood pressure (BP) (from $218.6{\pm}6.99$ to $190.0{\pm}3.42$ mm Hg, p<0.01). This result indicates that BSP is strong inducer of HO-1 expression, which may be triggered by oxidative stress, and has anti-hypertensive activity.

Keywords

References

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