YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Study on Extraction Condition and Analysis Methods of Benzopyrene in Black Ginseng

흑삼에서 벤조피렌의 추출 조건 및 분석 방법에 관한 연구

  • Cho, Young-Ho (Department of Pharmaceutical Engineering, Konyang University) ;
  • Song, Kyu-Yong (College of Pharmacy, Chungnam University) ;
  • Baek, Myoung-Ki (Life Science R&D Park, SK Biopharmaceuticals Co., LTD.) ;
  • Lee, Jong-Wha (Toxicology Center, Korea Institute of Toxicology) ;
  • Lee, Gye-Won (Department of Pharmaceutical Engineering, Konyang University)
  • Received : 2012.03.01
  • Accepted : 2012.06.26
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

To develop fundamental data of herbal materials with heat treatment, we studied analytical and extraction methods of benzopyrene in black ginseng and validated by HPLC analysis. Benzopyrene was successfully separated in mobile phase of acetonitrile:water (80 : 20) and detection of excitation 294 nm, emission 404 nm. The calibration curve of benzopyrene was linear over the concentration range of 1.17~37.50 ng/ml with correlation coefficient of above 0.999. The limit of detection (LOD) and quantitation (LOQ) of benzopyrene was 0.25 and 0.75 ng/ml, respectively. Hexane extraction method was used as a new extraction method for benzopyren and the efficient of extraction was over 95%. In conclusion, analytical method and extraction method were suitable for the determination of benzopyrene in the black ginseng and could be applied to fundamental study and guideline of herbal materials with heat treatment.

Keywords

References

  1. Columbia analytical services : U.S. EPA METHOD 610- Polynuclear Aromatic Hydrocarbons : Methods for organic chemical analysis of municipal and industrial waste water (1999).
  2. 식품의약품안전평가원 : 생약의 벤조피렌 시험법 해설서. p. 3 (2009)
  3. Shubik, P. and Hartwell, J. L. : Survey of compounds which have been tested for carcinogenic activity. Federal Security Agency, US Public Health Service, Washington, D. C. publication no. 149 (1957).
  4. Agency for Toxic Substances and Disease Registry (ATSDR) : Toxicological profile for polycyclic aromatic hydrocarbons (PAHs). U. S. Department of Health and Human Service, Public Health Service, p. 1 (1995).
  5. Ahlbom, A., Asma, S., Bhisey, R. A., Djordjevic, M. V., Gupta, P. C., Hecht, S. S., Idris, A. M., Jinot, J. and Nair, J. : IARC Monographs on the evaluation of carcinogenic risks to humans. World Health Organization International Agency for Research on Cancer 89 (2004).
  6. 식품의약품안전청 고시 제2008-51호 : 올리브유 중 벤조피렌 잔류 허용기준 및 시험법 (2008).
  7. 식품의약품안전청 고시 제2010-51호 : 수산물에 대한 벤조피렌 기준 및 규격 (2010).
  8. 식품의약품안전청 고시 제2010-75호 : 생약의 벤조피렌 기준 및 시험방법 (2010).
  9. Kim, N. J., Jung, E. A., Kim, H. J., Sim, S. B. and Kim, J. W. : Quality evaluation of various dried roots of Rehmannia glutinosa. Korean J. Pharmacogn. 31, 130 (2000).
  10. Chug, H. J. : Studies on variation of constituents and enzyme activities of Rehmannie Radix by Processing, Department of Pharmacy, Graduate School of Sookmyung Woman's University, p. 1 (1989).
  11. Lee, J. H., Shen, G. N., Kim, E. K., Shin, H. J., Myung, C. S., Oh, H. J., Kim, D. H., Roh, S. S., Cho, W., Seo, Y. B., Park, Y. J., Kang, C. W. and Song, G. Y. : Preparation of black ginseng and its antitumor activity. Korean J. Oriental Physiol. & Pathol. 20, 951 (2006).
  12. Song, G. Y., Roh, S. S., Seo, Y. B., Park, Y. J. and Myung, C. S. : Effect of Black Ginseng on Body Weight and Lipid Profiles. Yakhak Hoeji 50, 381 (2006).
  13. Han, S. T., Whang, W. K., Kim, I. H. and Yang, B. W. : Analysis of ginsenoside of black ginseng. Yakhak Hoeji 49, 490 (2005).
  14. Park, K. Y., Choi, S. B., Lee, G. C., Kim, N. S., Jeong, W. G., Koh, S. Y., Park, S. B., Choi, K. Y. and Cheong, E. H. : A study on the analytical method of monitoring compounds in drinking water(II)-chlorophenols, benzo($\alpha$)pyrene. Rep. Inst. Health & Environ. 12, 133 (2001).
  15. U. S. Pharmacopeia National Formulary, USP 26-NF 21, 3th Ed., Mack Printing Company, Easton, U. S. A., 2439 (2003).
  16. Beuving, G. : Validation of analytical Methods. Validation Seminar, Istambul, 31 May-01 June (2001).
  17. 한국 의약품 수출입 협회 : 한약재 중 벤조피렌 함유량 모니터링 연구, 식품의약품안전청 (2009).
  18. (주)글로벌 헬스 케어 생명과학연구소 : 한약재별 생약의 벤조피렌 시험법 검증 및 개선방안 연구, 식품의약품안전청 (2010).
  19. Shin, Y. J., Cheong, K, J., Lee, G. W., Lee, S. Y., Seo, Y. B. and Song, G. Y. : Study on new manufacturing method of Rehmanniae radix preparata and evaluation of antioxidant activity. Korean J. Aesthetics and Cosmetics Society 9, 199 (2011).