YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Stability of Citrate-capped Silver Nanoparticles in Isotonic Glycerol Solution for Intravenous Injection

글리세롤을 이용한 구연산캡핑 은나노입자의 정맥주사용 현탁액 조제 및 안정성

  • 이연진 (동덕여자대학교 약학대학) ;
  • 박광식 (동덕여자대학교 약학대학)
  • Received : 2012.02.18
  • Accepted : 2012.04.12
  • Published : 2012.04.30
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Abstract

Citrate-capped silver nanoparticles (AgNPs) are widely used in industry, consumer products and medical appliances. However, information on the toxicity, environmental fate and toxicokinetics are not enough. In this study, stability of citrate-capped AgNPs was investigated using different types of isotonic solution, which is important in the toxicokinetic study by the exposure route of intravenous injection. Size, morphology, zeta potential and ion formation were investigated in isotonic solutions for the physico-chemical characterization of AgNPs. Aggregation and precipitation of AgNPs were observed in saline or phosphate-buffered saline while they were stable without precipitation in 2% glycerol of isotonic solution. The average size of AgNPs in 2% glycerol was 6~10 nm, which was almost same as that in water-based suspension of AgNPs. Zeta potential was ranged from -30 mV to -60 mV, which was in the range of original stock AgNPs. The stability was maintained during the whole experimental period of 48 hours. Furthermore, the stability was not changed in different temperature (10~36$^{\circ}C$) and at different concentrations (10~1,000 ppm). The osmolarity of the AgNPs suspension was $299{\pm}1$ mOsm/kg which was in isotonic range. These data suggest that AgNPs in 2% glycerol solution can be used for the preparations of intravenous injection for toxicokinetic study without undesired disturbance of blood isotonicity.

Keywords

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