Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Anxiolytic-Like Effects of Chrysanthemum indicum Aqueous Extract in Mice: Possible Involvement of GABAA Receptors and 5-HT1A Receptors

  • Hong, Sa-Ik (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) ;
  • Kwon, Seung-Hwan (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) ;
  • Kim, Min-Jung (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) ;
  • Ma, Shi-Xun (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) ;
  • Kwon, Je-Won (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Choi, Seung-Min (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Choi, Soo-Im (YD Life Science Research Institutes, YD Life Science Co. Ltd.) ;
  • Kim, Sun-Yeou (College of Pharmacy, Gachon University) ;
  • Lee, Seok-Yong (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) ;
  • Jang, Choon-Gon (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)
  • Received : 2012.05.29
  • Accepted : 2012.07.04
  • Published : 2012.07.31
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Abstract

Chrysanthemum indicum Linne is an ancient herbal medicine used to treat bone and muscle deterioration, ocular inflammation, headache, and anxiety in Korea, China, and Japan. Furthermore, tea derived from Chrysanthemum indicum Linne has been used to treat anxiety by facilitating relaxation and curing insomnia. However, no reports exist on the anxiolytic-like effects of Chrysanthemum indicum Linne water extract (CWE) in mice. In the present study, we investigated the anxiolytic-like effects of CWE using the elevated plus-maze (EPM) test in mice. CWE, at a dose of 500 mg/kg (p.o.), significantly increased the time spent in the open arms of the EPM compared to a vehicle-injected control group. Moreover, the effect of CWE (500 mg/kg) was blocked by bicuculline (a selective $GABA_A$ receptor antagonist) and WAY 100635 (a selective 5-$HT_{1A}$ receptor antagonist). Taken together, these findings suggest that the anxiolytic-like effects of CWE might be mediated by the $GABA_A$ receptor and the 5-$HT_{1A}$ receptor.

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References

  1. Albert, C. M., Chae, C. U., Rexrode, K. M., Manson, J. E. and Kawachi, I. (2005) Phobic anxiety and risk of coronary heart disease and sudden cardiac death among women. Circulation 111, 480-487. https://doi.org/10.1161/01.CIR.0000153813.64165.5D
  2. Bailey, S. J. and Toth, M. (2004) Variability in the benzodiazepine response of serotonin 5-HT1A receptor null mice displaying anxietylike phenotype: evidence for genetic modifi ers in the 5-HT-mediated regulation of GABA(A) receptors. J. Neurosci. 24, 6343-6351. https://doi.org/10.1523/JNEUROSCI.0563-04.2004
  3. Bouayed, J., Rammal, H., Dicko, A., Younos, C. and Soulimani, R. (2007) Chlorogenic acid, a polyphenol from Prunus domestica (Mirabelle), with coupled anxiolytic and antioxidant effects. J. Neurol. Sci. 262, 77-84. https://doi.org/10.1016/j.jns.2007.06.028
  4. Cheeta, S., Kenny, P. J. and File, S. E. (2000) The role of 5-HT1A receptors in mediating the anxiogenic effects of nicotine following lateral septal administration. Eur. J. Neurosci. 12, 3797-3802. https://doi.org/10.1046/j.1460-9568.2000.00246.x
  5. Chen, X. Y., Li, J., Cheng, W. M., Jiang, H., Xie, X. F. and Hu, R. (2008) Effect of total fl avonoids of Chrysanthemum indicum on the apoptosis of synoviocytes in joint of adjuvant arthritis rats. Am. J. Chin. Med. 36, 695-704. https://doi.org/10.1142/S0192415X08006168
  6. Cheon, M. S., Yoon, T., Lee, do. Y., Choi G, Moon, B. C., Lee, A. Y., Choo, B. K. and Kim, H. K. (2009) Chrysanthemum indicum Linné extract inhibits the infl ammatory response by suppressing NFkappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages. J. Ethnopharmacol. 122, 473-477. https://doi.org/10.1016/j.jep.2009.01.034
  7. Clenet, F., Hascoet, M., Fillion, G., Galons, H. and Bourin, M. (2005) Role of GABA-ergic and serotonergic systems in the anxiolytic-like mechanism of action of a 5-HT-moduline antagonist in the mouse elevated plus maze. Behav. Brain Res. 158, 339-348. https://doi.org/10.1016/j.bbr.2004.09.015
  8. Collinson, N. and Dawson, G. R. (1997) On the elevated plus-maze the anxiolytic-like effects of the 5-HT(1A) agonist, 8-OH-DPAT, but not the anxiogenic-like effects of the 5-HT(1A) partial agonist, buspirone, are blocked by the 5-HT1A antagonist, WAY 100635. Psychopharmacology (Berl) 132, 35-43. https://doi.org/10.1007/s002130050317
  9. Cryan, J. F. and Holmes, A. (2005) The ascent of mouse: advances in modelling human depression and anxiety. Nat. Rev. Drug Discov. 4, 775-790. https://doi.org/10.1038/nrd1825
  10. Griebel, G., Rodgers, R. J., Perrault, G. and Sanger, D. J. (1999) Behavioural profi les in the mouse defence test battery suggest anxiolytic potential of 5-HT(1A) receptor antagonists. Psychopharmacology (Berl) 144, 121-130. https://doi.org/10.1007/s002130050984
  11. Heisler, L. K., Chu, H. M., Brennan, T. J., Danao, J. A., Bajwa, P., Parsons, L. H. and Tecott, L. H. (1998) Elevated anxiety and antidepressant- like responses in serotonin 5-HT1A receptor mutant mice. Proc. Natl. Acad. Sci. USA 95, 15049-15054. https://doi.org/10.1073/pnas.95.25.15049
  12. Jiang, X., Xing, G., Yang, C., Verma, A., Zhang, L. and Li, H. (2009) Stress impairs 5-HT2A receptor-mediated serotonergic facilitation of GABA release in juvenile rat basolateral amygdala. Neuropsychopharmacology 34, 410-423. https://doi.org/10.1038/npp.2008.71
  13. Kwon, S. H., Lee, H. K., Kim, J. A., Hong, S. I., Kim, H. C., Jo, T. H., Park, Y. I., Lee, C. K., Kim, Y. B., Lee, S. Y. and Jang, C. G. (2010) Neuroprotective effects of chlorogenic acid on scopolamineinduced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice. Eur. J. Pharmacol. 649, 210-217. https://doi.org/10.1016/j.ejphar.2010.09.001
  14. Lister, R. G. (1987) The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (Berl) 92, 180-185.
  15. Luscher, B., Fuchs, T. and Kilpatrick, C. L. (2011) GABAA receptor traffi cking-mediated plasticity of inhibitory synapses. Neuron 70, 385-409. https://doi.org/10.1016/j.neuron.2011.03.024
  16. Lydiard, R. B. (2003) The role of GABA in anxiety disorders. J. Clin. Psychiatry 64 Suppl 3, 21-27.
  17. Mello, E. L. Jr., Maior, R. S., Carey, R. J., Huston, J. P., Tomaz, C. and Muller, C. P. (2005) Serotonin1A-receptor antagonism blocks psychostimulant properties of diethylpropion in marmosets (Callithrix penicillata). Eur. J. Pharmacol. 511, 43-52. https://doi.org/10.1016/j.ejphar.2005.01.037
  18. Miller, S. M., Piasecki, C. C., Peabody, M. F. and Lonstein, J. S. (2010) GABA(A) receptor antagonism in the ventrocaudal periaqueductal gray increases anxiety in the anxiety-resistant postpartum rat. Pharmacol. Biochem. Behav. 95, 457-465. https://doi.org/10.1016/j.pbb.2010.03.007
  19. Peng, W. H., Wu, C. R., Chen, C. S., Chen, C. F., Leu, Z. C. and Hsieh, M. T. (2004) Anxiolytic effect of berberine on exploratory activity of the mouse in two experimental anxiety models: interaction with drugs acting at 5-HT receptors. Life Sci. 75, 2451-2462. https://doi.org/10.1016/j.lfs.2004.04.032
  20. Rodgers, R. J. and Cole, J. C. (1994) Anxiolytic-like effect of (S)-WAY 100135, a 5-HT1A receptor antagonist, in the murine elevated plus-maze test. Eur. J. Pharmacol. 26, 321-325.
  21. Rodgers, R. J. and Dalvi, A. (1997) Anxiety, defence and the elevated plus-maze. Neurosci. Biobehav. Rev. 21, 801-810. https://doi.org/10.1016/S0149-7634(96)00058-9
  22. Rudolph, U. and Möhler, H. (2006) GABA-based therapeutic approaches: GABAA receptor subtype functions. Curr. Opin. Pharmacol. 6, 18-23. https://doi.org/10.1016/j.coph.2005.10.003
  23. Shin, L. M. and Liberzon, I. (2010) The neurocircuitry of fear, stress, and anxiety disorders. Neuropsychopharmacology 35, 169-191. https://doi.org/10.1038/npp.2009.83
  24. Singh, Y. N. and Singh, N. N. (2002) Therapeutic potential of kava in the treatment of anxiety disorders. CNS. Drugs 16, 731-743. https://doi.org/10.2165/00023210-200216110-00002
  25. Smith, K. S., Engin, E., Meloni, E. G. and Rudolph, U. (2012) Benzodiazepine- induced anxiolysis and reduction of conditioned fear are mediated by distinct GABA(A) receptor subtypes in mice. Neuropharmacology 63, 250-258. https://doi.org/10.1016/j.neuropharm.2012.03.001
  26. Starr, K. R., Price, G. W., Watson, J. M., Atkinson, P. J., Arban, R., Melotto, S., Dawson, L. A., Hagan, J. J., Upton, N. and Duxon, M. S. (2007) SB-649915-B, a novel 5-HT1A/B autoreceptor antagonist and serotonin reuptake inhibitor, is anxiolytic and displays fast onset activity in the rat high light social interaction test. Neuropsychopharmacology 32, 2163-2172. https://doi.org/10.1038/sj.npp.1301341
  27. Tanaka, T., Nishikawa, A., Shima, H., Sugie, S., Shinoda, T., Yoshimi, N., Iwata, H. and Mori, H. (1990) Inhibitory effects of chlorogenic acid, reserpine, polyprenoic acid (E-5166), or coffee on hepatocarcinogenesis in rats and hamsters. Basic Life Sci. 52, 429-440.
  28. Walf, A. A. and Frye, C. A. (2007) The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat. Protoc. 2, 322-328. https://doi.org/10.1038/nprot.2007.44
  29. Yu, H. S., Lee, S. Y. and Jang, C. G. (2007) Involvement of 5-HT1A and GABAA receptors in the anxiolytic-like effects of Cinnamomum cassia in mice. Pharmacol. Biochem. Behav. 87, 164-170. https://doi.org/10.1016/j.pbb.2007.04.013

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