한국한의학연구원논문집 (Korean Journal of Oriental Medicine)

한국한의학연구원 (Korean Institute of Oriental Medicine)
권호 표지

시호 물 추출물의 마우스 골수세포를 이용한 유전독성 평가

Micronucleus Test of Bupleuri Radix Aqueous Extract in Bone Marrow Cells of Male ICR Mice

  • 전우현 (대구한의대학교 한의과대학) ;
  • 정인권 (대구한의대학교 한의과대학) ;
  • 강수진 (대구한의대학교 방제과학 글로벌 연구센터) ;
  • 구세광 (대구한의대학교 한의과대학) ;
  • 이영준 (대구한의대학교 한의과대학)
  • Cheon, Woo-Hyun (College of Oriental Medicine, Daegu Haany University) ;
  • Chung, In-Kwon (College of Oriental Medicine, Daegu Haany University) ;
  • Kang, Su-Jin (The Medical Research center for Globalization of Herbal Formulation, Daegu Haany University) ;
  • Ku, Sae-Kwang (College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Young-Joon (College of Oriental Medicine, Daegu Haany University)
  • 투고 : 2012.04.17
  • 심사 : 2012.07.20
  • 발행 : 2012.08.31
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초록

In this research, the genotoxic effect of Bupleuri Radix (BR), the dried roots of Bupleurum falcatum Linne has been traditionally used as anti-inflammatory agent, was evaluated using the mouse micronucleus test. BR aqueous extract (yield = 16.52%) was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2,000, 1,000 and 500 mg/kg. Cyclophosphamide (CPA) 70 mg/kg was used as a known genotoxic agent in a positive control. The appearance of a micronucleus (MN) in polychromatic erythrocyte (PCE) is used as an index for genotoxic potential, and PCE ratio is used as an index of cytotoxicity. Although significant (p<0.01) increase of the number of PCE with one or more nuclei (MNPCE) was detected in CPA treated groups, no significant increases of MNPCE numbers were observed in all three different dosages of BR extracts treated mice with over 0.30 of the individual polychromatic erythrocyte ratio in all mice used in this study. The results obtained indicated that BR extract shows no genotoxicity effects up to 2,000 mg/kg dosing levels the limit dosage in rodents.

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참고문헌

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