Korean Journal of Oriental Medicine (한국한의학연구원논문집)

Korean Institute of Oriental Medicine (한국한의학연구원)
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Effects of Apoptosis of Sophorae Radix on Human Gastric Adenocarcinoma cells

인체 위암세포에서 고삼의 세포사멸효과

  • Lim, Bo-Ra (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Lee, Hee-Jung (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Kim, Min-Chul (Division of Longevity and Biofunctional Medicine, Pusan National University) ;
  • Kim, Hyung-Woo (Division of Pharmacology School of Korean Medicine, Pusan National University) ;
  • Kim, Byung-Joo (Division of Longevity and Biofunctional Medicine, Pusan National University)
  • 임보라 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 이희정 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김민철 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김형우 (부산대학교 한의학전문대학원 약물의학부) ;
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학부)
  • Received : 2012.02.09
  • Accepted : 2012.03.26
  • Published : 2012.04.30
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Abstract

Objective : The purpose of this study was to investigate the anti-cancer effects of Sophorae Radix and the effects of 5-Fluorouracil (5-FU) in human gastric adenocarcinoma cells (AGS). Method : We used human gastric adenocarcinoma cell line, AGS cells. We examined cell death by MTT assay and caspase 3 assay with Sophorae Radix. To examine the inhibitory effects of Sophorae Radix, cell cycle (sub G1) analysis was done the AGS cells after three days with Sophorae Radix. The reversibility of Sophorae Radix was examined on one day to five days treatment with 100 ${\mu}g/ml$ Sophorae Radix. Result : Sophorae Radix inhibited the growth of AGS cells in a dose-dependent fashion. Also we showed that Sophorae Radix induced apoptosis in AGS cells by MTT assay, caspase 3 assay and sub-G1 analysis. Sophorae Radix combined with 5-FU markedly inhibited the growth of AGS cells compared to Sophorae Radix or 5-FU alone. After 3 days treatment of AGS cells with Sophorae Radix, the fraction of cells in sub-G1 phase was much higher than that of the control group. Conclusion : Our findings provide insight into unraveling the effects of Sophorae Radix in human gastric adenocarcinoma cells and developing therapeutic agents against gastric cancer.

Keywords

References

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