The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory effect of Rumex japonicus HOUTT. in RAW 264.7 cells

양제근(Rumex japonicus HOUTT.)분획의 항염증 효과

  • Huh, Jun-Yong (Dept. of Prescriptionology, College of Korean Medicine, Dongguk University) ;
  • Cho, Hyun-Jin (Dept. of Prescriptionology, College of Korean Medicine, Dongguk University) ;
  • Park, Ki-jeong (Dept. of Prescriptionology, College of Korean Medicine, Dongguk University) ;
  • Park, Sun-Dong (Dept. of Prescriptionology, College of Korean Medicine, Dongguk University)
  • 허준영 (동국대학교 한의과대학 방제학교실) ;
  • 조현진 (동국대학교 한의과대학 방제학교실) ;
  • 박기정 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Received : 2012.06.08
  • Accepted : 2012.06.27
  • Published : 2012.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The aim of this study was to authenticate whether fractionated extract of Rumex japonicus HOUTT. (RJ) has anti-inflammatory effects in mouse macrophage, RAW264.7 cells. Methods : Roots of RJ were extracted by methanol for 48hours. The methanol that gained was filtered and freeze dried. The methanol extract was dissolved in water and dichloromethane (DCM). After that, two layers were separated. Ethyl acetate (EA) added to the water layer and separated again. All the layers were filtered and freeze dried and the extracts were tested. Cytotoxic activity of extracts on RAW 264.7 cells was measured using MTS assay. The nitric oxide (NO) production was measured and proinflammatory cytokines and $PGE_2$ were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), I ${\kappa}$-B-${\alpha}$ and nuclear NF-${\kappa}B$ p65 expression were detected by western blot. Results : Our results indicated that DCM and EA extracts of RJ inhibited the LPS-induced NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of TNF-${\alpha}$, IL-$1{\beta}$ and IL-6 production in RAW 264.7 cells most effectively. DCM and EA extracts also had suppression effects of LPS-induced NF-${\kappa}B$ and MAPKs activation. Conclusions : This results demonstrate that fractionated extract of RJ has anti-inflammatory effects and among the fractioned extract, dichloromethane and ethyl acetate extract have best anti-inflammatory effects.

Keywords

References

  1. Hernandez-Ledesma B, Hsieh CC, de Lumen BO. Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages. Biochem Biophys Res Commun. 2009 ; 390(3) : 803-8. https://doi.org/10.1016/j.bbrc.2009.10.053
  2. College of Korean medicine, Professors of Herbology, collaborator. Herbology. Younglimsa, Seoul. 2000 ; 398-9
  3. Jang DS, Kim JM, Kim JH, Kim JS. 24-nor-Ursane type triterpenoids from the stems of Rumex japonicus. Chem Pharm Bull (Tokyo). 2005 ; 53(12) : 1594-6. https://doi.org/10.1248/cpb.53.1594
  4. Jiang L, Zhang S, Xuan L. Oxanthrone C-glycosides and epoxynaphthoquinol from the roots of Rumex japonicus. Phytochemistry. 2007 ; 68(19) : 2444-9. https://doi.org/10.1016/j.phytochem.2007.05.032
  5. Chen M, Wang D, Feng Y, Yang W. A new anthraquinone from roots of Rumex japonicus. Zhongguo Zhong Yao Za Zhi. 2009 ; 34(17) : 2194-6.
  6. Cho KH, Kim HS. A Study of Antifungal Activity with Rumex japonicus Houttuyn. The Korean Journal of dermaology. 1980 ; 18(5) : 383-90.
  7. Park YK. Studies on the Anti-oxidative effects of Rumexis Radix. Kor J Herbology. 2003 ; 18(4) : 269-77.
  8. Ahn JY, Im LR, Kim JH, Park JH, Kim DK, Lee YM. Effects of Rumecis Radix Water Extract on Development of Atopic Dermatitis in BALB/c Mice. Kor J Pharmacogn. 2009 ; 40(3) : 218-23.
  9. Lee HS, Kim SK, Han JB, Choi HM, Park JH, Kim EC, Choi MS, An HJ, Um JY, Kim HM, Min BI. Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice. Br J Dermatol. 2006 ; 155(1) : 33-8. https://doi.org/10.1111/j.1365-2133.2006.07303.x
  10. Desai A, Vyas T, Amiji M. Cytotoxicity and apopotosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J Pharm Sci. 2008 ; 97(7) : 2745-56. https://doi.org/10.1002/jps.21182
  11. Wang S, Chen Y, He D, He L, Yang Y, Chen J, Wang X. Inhibition of vascular smooth muscle cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation. J Ethnopharmacol. 2007 ; 114(3) : 458-62. https://doi.org/10.1016/j.jep.2007.08.039
  12. Moynagh PN. The NF-kappaB pathway. J Cell Sci. 2005 ; 118(Pt 20) : 4589-92. https://doi.org/10.1242/jcs.02579
  13. Kim MM, Kim SK. Effect of phloroglucinol on oxidative stress and inflammation. Food Chem Toxicol. 2010 ; 48(10) : 2925-33. https://doi.org/10.1016/j.fct.2010.07.029
  14. Park EK, Rhee HI, Jung HS, Ju SM, Lee YA, Lee SH, Hong SJ, Yang HI, Yoo MC, Kim KS. Antiinflammatory effects of a combined herbal preparation (RAH13) of Phellodendron amurense and Coptis chinensis in animal models of inflammation. Phytother Res. 2007 ; 21(8) : 746-50. https://doi.org/10.1002/ptr.2156
  15. Kim EH, Shim B, Kang S, Jeong G, Lee JS, Yu YB, Chun M. Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules. J Ethnopharmacol. 2009 ; 126(2) : 320-31. https://doi.org/10.1016/j.jep.2009.08.027
  16. Hwang SM, Lee YJ, Yoon JJ, Lee SM, Kang DG, Lee HS. Gardenia jasminoides inhibits tumor necrosis factor-alpha-induced vascular inflammation in endothelial cells. Phytother Res. 2010 ; 24(2) : 214-9. https://doi.org/10.1002/ptr.3099
  17. Gao Q, Wang F, Guo S, Li J, Zhu B, Cheng J, Jin Y, Li B, Wang H, Shi S, Gao Q, Zhang Z, Cao W, Tian Y. Sonodynamic effect of an anti-inflammatory agent-emodin on macrophages. Ultrasound Med Biol. 2011 ; 37(9) : 1478-85. https://doi.org/10.1016/j.ultrasmedbio.2011.05.846
  18. Abdelwahab SI, Hassan LE, Sirat HM, Yagi SM, Koko WS, Mohan S, Taha MM, Ahmad S, Chuen CS, Narrima P, Rais MM, Hadi AH. Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: Role of reactive nitrogen species and cyclooxygenase enzyme inhibition. Fitoterapia. 2011 ; 82(8) : 1190-7. https://doi.org/10.1016/j.fitote.2011.08.002
  19. Kirii H, Niwa T, Yamada Y, Wada H, Saito K, Iwakura Y, Asano M, Moriwaki H, Seishima M. Lack of interleukin-1beta decreases the severity of atherosclerosis in ApoE-deficient mice. Arterioscler Thromb Vasc Biol. 2003 ; 23(4) : 656-60. https://doi.org/10.1161/01.ATV.0000064374.15232.C3
  20. Sporn MB, Liby KT, Yore MM, Fu L, Lopchuk JM, Gribble GW. New synthetic triterpenoids: potent agents for prevention and treatment of tissue injury caused by inflammatory and oxidative stress. J Nat Prod. 2011 ; 74(3) : 537-45. https://doi.org/10.1021/np100826q
  21. Lee KC, Chang HH, Chung YH, Lee TY. Andrographolide acts as an anti-inflammatory agent in LPS-stimulated RAW264.7 macrophages by inhibiting STAT3-mediated suppression of the NF-$\kappa B$ pathway. J Ethnopharmacol. 2011 ; 135(3) : 678-84. https://doi.org/10.1016/j.jep.2011.03.068
  22. Jedinak A, Dudhgaonkar S, Wu QL, Simon J, Sliva D. Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-$\kappa B$ and AP-1 signaling. Nutr J. 2011 ; 10 : 52. https://doi.org/10.1186/1475-2891-10-52
  23. Lim C, Lee M, Park EJ, Cho R, Park HJ, Lee SJ, Cho H, Lee SK, Kim S. Sulfonamide derivatives of styrylheterocycles as a potent inhibitor of COX-2-mediated prostaglandin E2 production. Bioorg Med Chem Lett. 2010 ; 20(23) : 6938-41. https://doi.org/10.1016/j.bmcl.2010.09.136
  24. Himaya SW, Ryu B, Qian ZJ, Kim SK. Sea cucumber, Stichopus japonicus ethyl acetate fraction modulates the lipopolysaccharide induced iNOS and COX-2 via MAPK signaling pathway in murine macrophages. Environ Toxicol Pharmacol. 2010 ; 30(1) : 68-75. https://doi.org/10.1016/j.etap.2010.03.019
  25. Bonizzi G, Karin M. The two NF-kappaB activation pathways and their role in innate and adaptive immunity. Trends Immunol. 2004 ; 25(6) : 280-8. https://doi.org/10.1016/j.it.2004.03.008
  26. Hosoda H, Tamura H, Kida S, Nagaoka I. Transcriptional regulation of mouse TREM-1 gene in RAW264.7 macrophage-like cells. Life Sci. 2011 ; 89(3-4) : 115-22. https://doi.org/10.1016/j.lfs.2011.05.007
  27. Cho YC, Lee SH, Yoon G, Kim HS, Na JY, Choi HJ, Cho CW, Cheon SH, Kang BY. Licochalcone E reduces chronic allergic contact dermatitis and inhibits IL-12p40 production through down-regulation of NF-kappa B. Int Immunopharmacol. 2010 ; 10(9) : 1119-26. https://doi.org/10.1016/j.intimp.2010.06.015
  28. Chen SR, Xu XZ, Wang YH, Chen JW, Xu SW, Gu LQ, Liu PQ. Icariin derivative inhibits inflammation through suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB pathways. Biol Pharm Bull. 2010 ; 33(8) : 1307-13. https://doi.org/10.1248/bpb.33.1307
  29. Tang S, Shen XY, Huang HQ, Xu SW, Yu Y, Zhou CH, Chen SR, Le K, Wang YH, Liu PQ. Cryptotanshinone suppressed inflammatory cytokines secretion in RAW264.7 macrophages through inhibition of the NF-$\kappa B$ and MAPK signaling pathways. Inflammation. 2011 ; 34(2) : 111-8. https://doi.org/10.1007/s10753-010-9214-3