대한본초학회지 (The Korea Journal of Herbology)

  • 제27권4호
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  • Pages.73-80
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  • 2012
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과

Inhibitory Effects of Marine Natural Products on Melanogenesis in B16 Melanoma Cells

  • 이찬 (계명대학교 의과대학 약리학교실) ;
  • 장정희 (계명대학교 의과대학 약리학교실) ;
  • 안은미 (대구한의대학교 한방식품약리학과) ;
  • 박찬익 (대구한의대학교 화장품약리학과)
  • Lee, Chan (Department of Pharmacology, School of Medicine, Keimyung University) ;
  • Jang, Jung-Hee (Department of Pharmacology, School of Medicine, Keimyung University) ;
  • Ahn, Eun-Mi (Department of Herbal Food Pharmacology, Daegu Haany University) ;
  • Park, Chan-Ik (Department of Cosmeceutical Science, Daegu Haany University)
  • 투고 : 2012.05.13
  • 심사 : 2012.06.28
  • 발행 : 2012.07.30
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초록

Objectives : Under normal condition melanin protects the skin from extracellular stimuli including ultraviolet (UV)-induced oxidative skin damages, but excess production and accumulation of melanin can induce hyperpigmentation causing esthetic problems. Therefore, in this study we tried to search for natural skin whitening materials from marine natural resources. Methods : Water and ethanol extracts of marine natural resources were prepared from Porphyra thalli (PT), Laminariae thallus (LT), Ostreae concha (OC), Sargassum thallus (ST), Undaria thallus (UT), Codium thalli (CT), Enteromorpha thalli (ET), Syngnathoides biaculeatus (SB), and Hippocampus coronatus (Hc). Their effects against UVB and ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH)-induced melanogenesis were investigated based on melanin formation in B16 mouse melanoma cells. The mRNA and protein expression of enzymes involved in the melanogenic process were further examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Results : Water extract of Ostreae concha (OCW/E) effectively inhibited UVB and ${\alpha}$-MSH-induced melanin production in B16 melanocytes, which seemed to be mediated by inhibition of mRNA expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). In another experiment, ethanol extracts from Porphyra thalli (PTE/E), Laminariae thallus (LTE/E), Sargassum thallus (STE/E), Undaria thallus (UTE/E), Codium thalli (CTE/E), Syngnathoides biaculeatus (SBE/E), and Hippocampus coronatus (HcE/E) significantly suppressed UVB and ${\alpha}$-MSH-induced melanin formation. Furthermore, ethylacetate fraction isolated form LTE/E (LTE/EEt) decreased UVB and ${\alpha}$-MSH-elevated extracellular melanin levels via inhibition of tyrosinase protein expression. Conclutions : These results suggest that marine natural resources such as Porphyra thalli, Laminariae thallus, Ostreae concha, Sargassum thallus, Undaria thallus, Codium thalli, Syngnathoides biaculeatus and Hippocampus coronatus have anti-melanogenic effects, thereby exhibiting high potentials to be utilized as one of the ingredients for the development of new whitening functional cosmetics.

키워드

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