Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Genetic Diversity of Culturable Endophytic Fungi Isolated from Halophytes Naturally Growing in Muan Salt Marsh

무안 염습지에 자생하는 염생식물(halophyte)의 뿌리로부터 분리된 내생진균(endophytic fungi)의 유전학적 다양성

  • You, Young-Hyun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Seo, Yeong-Gyo (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kim, Mi-Ae (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kang, Myung-Suk (National Institute of Biological Resource) ;
  • Kim, Chang-Mu (National Institute of Biological Resource) ;
  • Ha, Sang-Chul (Department of Confectionary and Decoration, Daegu Mirae college) ;
  • Cho, Ga-Youn (National Institute of Biological Resource) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
  • 유영현 (경북대학교 생명과학부) ;
  • 윤혁준 (경북대학교 생명과학부) ;
  • 서영교 (경북대학교 생명과학부) ;
  • 김미애 (경북대학교 생명과학부) ;
  • 강명석 (국립생물자원관 미생물자원과) ;
  • 김창무 (국립생물자원관 미생물자원과) ;
  • 하상철 (대구미래대학교 제과데코레이션과) ;
  • 조가연 (국립생물자원관 미생물자원과) ;
  • 김종국 (경북대학교 생명과학부)
  • Received : 2012.05.12
  • Accepted : 2012.07.19
  • Published : 2012.07.30
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Abstract

Native halophytes, such as Suaeda maritima, Limonium tetragonum, S. japonica, Zoysia sinica, and Phragmites australis were collected from the Muan salt marsh. Ninety endophytic fungi were isolated from the roots of the collected halophytes. Molecular insights inferred by internal transcribed spacer containing ITS1, 5.8s, and the ITS2 region showed that all the fungal strains belong to ten orders, i.e., Capnodiales (4.44%), Cystofilobasidiales (1.11%), Dothideales (3.33%), Eurotiales (53.33%), Glomerellales (3.33%), Hypocreales (8.89%), Mucorales (1.11%), Pleosporales (15.56%), Sordariales (1.11%), and Trichosphaeriales (1.11%). The rest (6.67%) of all fungal isolates were not identified. Ninety fungal strains were confirmed at the genus level, containing Acremonium, Alternaria, Aspergillus, Aureobasidium, Cephalosporium, Chaetomium, Cladosporium, Colletotrichum, Cryptococcus, Didymella, Dothideomycete, Emericellopsis, Epicoccum, Eupenicillium, Fusarium, Gibberella, Gongronella, Macrophoma, Microsphaeropsis, Nigrospora, Paecilomyces, Paraconiothyrium, Penicillium, Phaeomyces, Phoma, Pleosporales, Purpureocillium, and Talaromyces. Of all the endophytic fungi identified from the various halophytes, Aspergillus and Penicillium of Eurotiales had the highest abundance.

90종의 내생진균(endophytic fungi)은 염습지에 자생하고 있는 염생식물(halophyte)의 뿌리에서 분리하였다. 자생식물 샘플은 해홍나물, 갯질경, 칠면초, 갯잔디, 갈대가 염습지로부터 분리하였다. 그리고 분리된 내생진균들은 ITS1, 5.8S와 ITS2를 포함하는 ITS-rDNA 영역에 의해 분석하였다. 다양한 내생진균은 Capnodiales (4.44%), Cystofilobasidiales (1.11%), Dothideales (3.33%), Eurotiales (53.33%), Glomerellales (3.33%), Hypocreales (8.89%), Mucorales (1.11%), Pleosporales (15.56%), Sordariales (1.11%), Trichosphaeriales (1.11%), unidentified (6.67%) 등 10종류 목에 속하는 것이 확인되었다. 90종의 내생진균은 속(genus)단계에서 Acremonium 속, Alternaria 속, Aspergillus 속, Aureobasidium 속, Cephalosporium 속, Chaetomium 속, Cladosporium 속, Colletotrichum 속, Cryptococcus 속, Didymella 속, Dothideomycete 속, Emericellopsis 속, Epicoccum 속, Eupenicillium 속, Fusarium 속, Gibberella 속, Gongronella 속, Macrophoma 속, Microsphaeropsis 속, Nigrospora 속, Paecilomyce 속, Paraconiothyrium 속, Penicillium 속, Phaeomyces 속, Phoma 속, Pleosporales 속, Purpureocillium 속, 그리고 Talaromyces 속으로 모두 28속이 확인되었다. 그리고 동정된 모든 내생진균은 Eurotiales목의 Aspergillus속과 Penicillium속이 가장 많이 분포하고 있는 것이 확인되었다.

Keywords

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