생명과학회지 (Journal of Life Science)

  • 제22권7호
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  • Pages.970-980
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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무안 염습지에 자생하는 염생식물(halophyte)의 뿌리로부터 분리된 내생진균(endophytic fungi)의 유전학적 다양성

Genetic Diversity of Culturable Endophytic Fungi Isolated from Halophytes Naturally Growing in Muan Salt Marsh

  • 유영현 (경북대학교 생명과학부) ;
  • 윤혁준 (경북대학교 생명과학부) ;
  • 서영교 (경북대학교 생명과학부) ;
  • 김미애 (경북대학교 생명과학부) ;
  • 강명석 (국립생물자원관 미생물자원과) ;
  • 김창무 (국립생물자원관 미생물자원과) ;
  • 하상철 (대구미래대학교 제과데코레이션과) ;
  • 조가연 (국립생물자원관 미생물자원과) ;
  • 김종국 (경북대학교 생명과학부)
  • You, Young-Hyun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Seo, Yeong-Gyo (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kim, Mi-Ae (Department of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kang, Myung-Suk (National Institute of Biological Resource) ;
  • Kim, Chang-Mu (National Institute of Biological Resource) ;
  • Ha, Sang-Chul (Department of Confectionary and Decoration, Daegu Mirae college) ;
  • Cho, Ga-Youn (National Institute of Biological Resource) ;
  • Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
  • 투고 : 2012.05.12
  • 심사 : 2012.07.19
  • 발행 : 2012.07.30
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초록

90종의 내생진균(endophytic fungi)은 염습지에 자생하고 있는 염생식물(halophyte)의 뿌리에서 분리하였다. 자생식물 샘플은 해홍나물, 갯질경, 칠면초, 갯잔디, 갈대가 염습지로부터 분리하였다. 그리고 분리된 내생진균들은 ITS1, 5.8S와 ITS2를 포함하는 ITS-rDNA 영역에 의해 분석하였다. 다양한 내생진균은 Capnodiales (4.44%), Cystofilobasidiales (1.11%), Dothideales (3.33%), Eurotiales (53.33%), Glomerellales (3.33%), Hypocreales (8.89%), Mucorales (1.11%), Pleosporales (15.56%), Sordariales (1.11%), Trichosphaeriales (1.11%), unidentified (6.67%) 등 10종류 목에 속하는 것이 확인되었다. 90종의 내생진균은 속(genus)단계에서 Acremonium 속, Alternaria 속, Aspergillus 속, Aureobasidium 속, Cephalosporium 속, Chaetomium 속, Cladosporium 속, Colletotrichum 속, Cryptococcus 속, Didymella 속, Dothideomycete 속, Emericellopsis 속, Epicoccum 속, Eupenicillium 속, Fusarium 속, Gibberella 속, Gongronella 속, Macrophoma 속, Microsphaeropsis 속, Nigrospora 속, Paecilomyce 속, Paraconiothyrium 속, Penicillium 속, Phaeomyces 속, Phoma 속, Pleosporales 속, Purpureocillium 속, 그리고 Talaromyces 속으로 모두 28속이 확인되었다. 그리고 동정된 모든 내생진균은 Eurotiales목의 Aspergillus속과 Penicillium속이 가장 많이 분포하고 있는 것이 확인되었다.

Native halophytes, such as Suaeda maritima, Limonium tetragonum, S. japonica, Zoysia sinica, and Phragmites australis were collected from the Muan salt marsh. Ninety endophytic fungi were isolated from the roots of the collected halophytes. Molecular insights inferred by internal transcribed spacer containing ITS1, 5.8s, and the ITS2 region showed that all the fungal strains belong to ten orders, i.e., Capnodiales (4.44%), Cystofilobasidiales (1.11%), Dothideales (3.33%), Eurotiales (53.33%), Glomerellales (3.33%), Hypocreales (8.89%), Mucorales (1.11%), Pleosporales (15.56%), Sordariales (1.11%), and Trichosphaeriales (1.11%). The rest (6.67%) of all fungal isolates were not identified. Ninety fungal strains were confirmed at the genus level, containing Acremonium, Alternaria, Aspergillus, Aureobasidium, Cephalosporium, Chaetomium, Cladosporium, Colletotrichum, Cryptococcus, Didymella, Dothideomycete, Emericellopsis, Epicoccum, Eupenicillium, Fusarium, Gibberella, Gongronella, Macrophoma, Microsphaeropsis, Nigrospora, Paecilomyces, Paraconiothyrium, Penicillium, Phaeomyces, Phoma, Pleosporales, Purpureocillium, and Talaromyces. Of all the endophytic fungi identified from the various halophytes, Aspergillus and Penicillium of Eurotiales had the highest abundance.

키워드

참고문헌

  1. Arnold, E. A., Mejia, L. C., Kyllo, D., Rojas, E., Maynard, Z., Robbins, N. and Herre, E. A. 2003. Fungal endophytes limit pathogen damage in a tropical tree. Proc. Natl. Acad. Sci. USA 100, 15649-15654. https://doi.org/10.1073/pnas.2533483100
  2. Chapman, V. J. 1974. Salt Marshes and Salt Deserts of the World. In Ecology of Halophytes. pp. 3-22, Academic Press, New York.
  3. Gomes, N. C. M., Cleary, D. F. R., Pinto, F. N., Egas, C., Almeida, A., Cunha, A., Mendonça-Hagler, L. C. S. and Smalla, K. 2010. Taking root: enduring effect of rhizosphere bacterial colonization in mangroves. PLoS ONE 5, e14065. https://doi.org/10.1371/journal.pone.0014065
  4. Hamayun, M., Khan, S. A., Khan, M. A., Khan, A. L., Kang, S. M., Kim, S. K., Joo, G. J. and Lee, I. J. 2009. Gibberellin production by pure cultures of a new strain of Aspergillus fumigates. World J. Microbiol. Biotechnol. 25, 1785-1792. https://doi.org/10.1007/s11274-009-0078-3
  5. Khan, A. L., Hamayun, M., Ahmad, N., Hussain, J., Kang, S. M., Kim, Y. H., Adnan, M. D., Tang, S., Waqas, M., Radhakrishnan, R., Hwang, Y. H. and Lee, I. J. 2011. Salinity Stress Resistance Offered by Endophytic Fungal Interaction Between Penicillium minioluteum LHL09 and Glycine max. L. J. Microbiol. Biotechnol. 21, 893-902. https://doi.org/10.4014/jmb.1103.03012
  6. Khan, A. L., Hamayun, M., Kang, S. M., Kim, Y. H., Jung, H. Y., Lee, J. H. and Lee, I. J. 2012. Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10. BMC Microbiol. 12, 3. https://doi.org/10.1186/1471-2180-12-3
  7. Kim, B. S., Oh, H. M., Kang, H., Park, S. and Chun, J. 2004. Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J. Microbiol. Biotechnol. 14, 205-211.
  8. Khan, S. A., Hamayun, M., Rim, S. O., Lee, I. J., Seu, J. C., Choo, Y. S., Jin, I. N., Kim, S. D., Lee, I. K. and Kim, J. G. 2008. Isolation of endophytic fungi capable of plant growth promotion from monocots inhibited in the coastal sand dunes of Korea. J. Life Sci. 18, 1355-1359. https://doi.org/10.5352/JLS.2008.18.10.1355
  9. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Lee, I. J. and Kim, J. G. 2009. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J. Microbiol. Biotechnol. 25, 829-833. https://doi.org/10.1007/s11274-009-9981-x
  10. Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diveristy of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Korean J. Mycol. 37, 130-133. https://doi.org/10.4489/KJM.2009.37.2.130
  11. Mohamed, D. J. and Martiny, J. B. 2011. Patterns of fungal diversity and composition along a salinity gradient. ISME J. 5, 379-88. https://doi.org/10.1038/ismej.2010.137
  12. Park, K. H. and Lee, H. K. 2006. Establishment of the wetland ecosystem information system based on Web-GIS in Gyeongnam region. J. Environ. Res. 6, 99-103.
  13. Pielou, E. C. 1975. Ecological diversity. p 165. John Wiley, New York.
  14. Redman, R. S., Sheehan, K. B., Stout, R. G., Rodriguez, R. J. and Henson, J. M. 2002. Thermotolerance conferred to plant host and fungal endophyte during mutualistic symbiosis. Science 298, 1581. https://doi.org/10.1126/science.1072191
  15. Rodriguez, R. J., Redman, R. S. and Henson, J. M. 2004. The role of fungal symbioses in the adaptation of plants to high stress environments. Mitigation and Adaptation Strategies for Global Change 9, 261-272. https://doi.org/10.1023/B:MITI.0000029922.31110.97
  16. Rodriguez, R. J., Henson, J., Van, V. E., Hoy, M., Wright, L., Beckwith, F., Kim, Y. and Redman, R. S. 2008. Stress tolerance in plants via habitat-adapted symbiosis. ISME J. 2, 404-416. https://doi.org/10.1038/ismej.2007.106
  17. Seo, S. T., Kim, K. H., Kim, M. J., Hong, J. S., Park, J. H. and Shin, S. C. 2009. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Korean J. Mycol. 37, 108-110. https://doi.org/10.4489/KJM.2009.37.1.108
  18. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Bio. Evol. 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  19. Vazquez M. M., Cesar, S., Azcon, R. and Barea, J. M. 2000. Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl. Soil Ecol. 15, 261-272. https://doi.org/10.1016/S0929-1393(00)00075-5
  20. Waller, F., Achatz, B., Baltruscha, T. H., Fodor, J., Becker, K., Fischer, M., Heier, T., Hckelhoven, R., Neumann, C., Wettstein, D. V., Franken, P. and Kogel, K. H. 2005. The endophytic fungus Piriformospora indica reprograms barley to saltstress tolerance, disease resistance, and higher yield. Proc. Natl. Acad. Sci. USA 102, 13386-13391. https://doi.org/10.1073/pnas.0504423102
  21. Yamada, A., Takeo, O., Yosuke, D. and Masatake, O. 2001. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience 42, 43-50. https://doi.org/10.1007/BF02463974
  22. You, Y. H., Yoon, H., Lee, G. S., Woo, J. R., Shin, J. H., Lee, I. J., Rim, S. O., Choo, Y. S. and Kim, J. G. 2011. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. J. Life Sci. 21, 992-996. https://doi.org/10.5352/JLS.2011.21.7.992
  23. You, Y. H., Yoon, Woo, J. R. Seo, Y., Kim, M. Lee, G. and Kim, J. G. 2012. Diversity of endophytic fungi from the roots of halophytes growing in go-chang salt marsh. Korean J. Mycol. 40, 86-92. https://doi.org/10.4489/KJM.2012.40.2.86
  24. Zhang, X. Y., Bao, J. G., Wang, H., He, F., Xu, X. Y. and Qi, S. H. 2012. Diversity and Antimicrobial activity of culturable fungi isolated from six species of the South China sea gorgonians. Microbiol. Ecol. In press. DOI 10.1007/s00248-012-0050-x.

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  4. Endophytic Fungal Diversity Isolated from the Root of Halophytes in Taean Peninsula vol.42, pp.4, 2014, https://doi.org/10.4489/KJM.2014.42.4.269
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