Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Xylanase properties of Bacillus subtilis AB-55 isolated from waste mushroom bed of Agaricus bisporus

양송이 수확 후 배지로부터 분리한 Bacillus subtilis AB-55가 생산하는 xylanase의 특성

  • Choi, Won-Ho (Department of Bio-Enviromental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Yong-Su (Department of Bio-Enviromental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Min-Ho (Department of Bio-Enviromental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • 최원호 (충남대학교 농업생명과학대학 생물환경 화학과) ;
  • 최용수 (충남대학교 농업생명과학대학 생물환경 화학과) ;
  • 장갑열 (국립원예특작과학원 버섯과) ;
  • 윤민호 (충남대학교 농업생명과학대학 생물환경 화학과)
  • Received : 2012.05.18
  • Accepted : 2012.06.18
  • Published : 2012.06.30
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Abstract

A bacterium AB-55, isolated from waste mushroom bed of Agaricus bisporus in Sukseong-myeon, Buyeo-gun, Chungcheongnam-do, Korea, was screened onto xylan agar congo-red plate by the xylanolysis method and was used to produce an xylanase in shaker buffle flask cultures containing oat spelt xylans. The phylogenetic analysis using 16S rRNA gene sequence data showed that the strain AB-55 had the highest homology (99.0%) with Bacillus subtilis and it was named as Bacillus subtilis AB-55. A xylanase was purified by ammonium sulfate precipitation (50~80%), gel filtration on sephacryl S-300, and ion exchange chromatography on DEAE sepharose FF. The molecular weight of the xylanase was estimated as 44 kDa by SDS-PAGE. Optimal pH and temperature for the xylanase activity was pH 7 and $50^{\circ}C$, respectively. N-terminal amino acid sequence of the enzyme was identified as Ser-Ala-Val-Lys-His-Gly-Ala-Ile-Val-Phe. The substrate specificity of the enzyme exhibited that it hydrolyzed efficiently oat spelt xylan as well as beechwood xylan, but showed no activity against Avicel and carboxymethyl clellulose (CMC). The enzyme activity was enhanced by $Fe^{2+}$ and $Mn^{2+}$ whereas was entirely inhibited by $Hg^+$.

Keywords

References

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