Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지

Distribution of Mouse Uterine Mast Cells during Estrous Cycle

발정주기에 따른 생쥐 자궁조직 내 비만세포의 분포

  • Received : 2012.04.13
  • Accepted : 2012.05.24
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

We examined the distribution of uterine mast cells in cycling mice of different ages between 7 weeks and 38 weeks by toluidine blue staining. Mast cell density increased gradually depending on the age and culminated at 30 weeks after birth. Of the estrous stages, uteruses at metestrus showed markedly higher density of mast cells than those at the other stages in cycling mice of all ages analysed in this study and the majority of the mast cells were found in myometrium. We also discriminated types of the uterine mast cells in cycling mice at 10 weeks old by Alcian blue-safranin double staining. Mucosal type was most abundant among three types, including connective-tissue types and mixed types, through the entire estrous cycles. The portion of mixed types increased slightly after estrous. The abundance of uterine collagen protein determined by Masson trichrome staining was exactly consistent with the density distribution of the uterine mast cells during estrous cycles. Taken together, these results may imply that uterine mucosal mast cells, together with collagen proteins, are heavily involved in tissue remodeling of cycling mouse uterus.

정상 발정주기를 보이는 7주령부터 38주령의 생쥐를 대상으로 발정주기에 따른 자궁조직내 비만세포의 분포를 toluidine blue 염색법으로 조사하였다. 비만세포의 밀도는 생쥐의 연령 증가와 더불어 지속적으로 증가하다가, 30주령 이후 감소하는 경향을 보였다. 발정주기별 분포에서는 조사한 모든 연령의 생쥐에서 발정후기에 가장 높은 밀도를 나타내었고, 그 대부분은 자궁근층에서 발견되었다. 10주령의 생쥐를 대상으로 Alcian blue-safranin 이중염색법에 따라 비만세포의 유형별 상대분포를 조사한 실험에서는 발정주기의 전 시기에 걸쳐 점막형 비만세포의 비율이 결합조직형 및 혼합형 비만세포의 비율에 비해 상대적으로 현저히 높게 나타났으나, 발정기 이후 발정간기까지는 점막형비만세포의 상대 비율은 다소 감소하고, 다른 유형의 비만세포, 특히 혼합형 비만세포의 비율이 상대적으로 증가하는 경향을 보였다. Masson trichrome 염색법으로 조사한 교원섬유의 분포 양상은 비만세포의 분포 양상과 정확히 일치하게 발정후기에서 가장 높은 밀도를 나타내었다. 이상의 결과는 비만세포, 특히 점막형 비만세포가 교원섬유와 함께 발정주기에 따른 자궁의 점막 및 근육조직의 재구성에 중요한 역할을 하고 있음을 시사한다.

Keywords

References

  1. Aydin Yasemin, Nese Tuncel, Firdevs Gurer, Muzaffer Tuncel, MuberraKosar, Gul Oflaz (1998) Ovarian, uterine and brain mast cells in female rats: Cyclic changes and contribution to tissue histamine. Comparative Biochemi and Physiol Part A 120. 255-262. https://doi.org/10.1016/S1095-6433(98)00027-0
  2. Bachelet CM, Bernaudin JF, Fleury-Feith J (1988) Distribution and histochemical characterization of pulmonary mast cells in the rat and guinea pig. Int Arch Allergy Appl Immunol 87(3):225-229. https://doi.org/10.1159/000234677
  3. Benoist C, Mathis D (2002) Mast cells in autoimmune disease. Nature 19-26;420(6917):875-878. https://doi.org/10.1038/nature01324
  4. Bytautiene E, Vedernikov YP, Saada GR, Romero R, Garfield R (2008) IgE-independent mast cell activation augments contractility of nonpregnant and pregnant guinea pig myometrium. Int Arch Allergy Immunol. 147(2):140-146. https://doi.org/10.1159/000135701
  5. Caligioni CS (2009) Assessing reproductive status/stages in mice. Curr Protoc Neurosci Appendix 4:Appendix 4I.
  6. Chen Z, Irani AA, Bradford TR, Craig SS, Newlands G, Miller H, Huff T, Simmons WH, Schwartz LB (1993) Localization of rat tryptase to a subset of the connective tissue type of mast cell. J Histochem Cytochem 41(7):961-969. https://doi.org/10.1177/41.7.7685789
  7. Church MK, Levi-Schaffer F (1997) The human mast cell. J Allergy Clin Immunol 99:155-160. https://doi.org/10.1016/S0091-6749(97)70089-7
  8. el Sayed So, Dyson M(1993) Histochemical heterogeneity of mast cells in rat dermis. Biotech Histochem 68(6): 326-332. https://doi.org/10.3109/10520299309105638
  9. Galli SI, Tsai M (2008) Mast cells: versatile regulators of inflammation, tissue remodeling, host defense and homeostasis. J Dermatol Sci 49(1):7-19. https://doi.org/10.1016/j.jdermsci.2007.09.009
  10. Garfield RE, Irani AM, Schwartz LB, Bytautiene E and Romero R (2006) Structural and functional comparison of mast cells in the pregnant versus nonpregnant human uterus. Am J Obstet Gynecol 194(1):261-267. https://doi.org/10.1016/j.ajog.2005.05.011
  11. Gaytan F, Aceitero J, Bellido C, Sanchez-Criado JE, Aguilar E (1991) Estrous cycle-related changes in mast cell numbers in several ovarian compartments in the rat. Biology of Reprod 45:27-33. https://doi.org/10.1095/biolreprod45.1.27
  12. Hatamochi A, Fujiwara K, Ueki H(1985) Effects of histamine on collagen synthesis by cultured fibroblasts derived from guinea pig skin. Arch Dermatol Res 277(1):60-64.
  13. Jensen F, Woudwyk M, Teles A, Woidacki K, Taran F, Costa S, Malfertheiner SF, Zenclussen AC (2010) Estradiol and progesterone regulate the migration of mast cells from the periphery to the uterus and induce their maturation and degranulation. PLOS One 22;5(12): e14409. https://doi.org/10.1371/journal.pone.0014409
  14. Karaca T, Arikan S, Kalender H, Yoruk M (2008) Distribution and heterogeneity of mast cells in female reproductive tract and ovary on different days of the oestrus cycle in Angora goats. Reprod Domest Anim 43(4):451-456. https://doi.org/10.1111/j.1439-0531.2007.00934.x
  15. Krishnaswamy G, Ajitawi O, and Chi DS (2006) The human mast cell-an overview in Mast Cells: Methods and Protocols Edited by: G. Krishnaswamy and D. S. Chi Methods in Molecular Biology, vol. 315.
  16. Marone G, Triggiani M, Genovese A, De Paulis A (2005) Role of human mast cells and basophils in bronchial asthma. Adv Immunol 88:97-160.
  17. Mekori YA, Metcalfe DD (2000) Mast cells in innate immunity. Immunol Rev 173:131-140. https://doi.org/10.1034/j.1600-065X.2000.917305.x
  18. Menzies FM, Shepherd MC, Nibbs RJ, Nelson SM (2011) The role of mast cells and their mediators in reproduction, pregnancy and labour. Hum Reprod Update 17(3):383-396. https://doi.org/10.1093/humupd/dmq053
  19. Metcalfe DD, Baram D, Mekori YA (1997) Mast cells. Physiol Rev 77(4):1033-1079. https://doi.org/10.1152/physrev.1997.77.4.1033
  20. Metz M, Maurer M (2007) Mast cells--key effector cells in immune responses. Trends Immunol 28(5):234-241. https://doi.org/10.1016/j.it.2007.03.003
  21. Monika Welle (1997) Development, significance, and heterogeneity of mast cells with particular regard to the mast cell-specific proteases chymase and tryptase. Journal of Leuko Biol 61:233. https://doi.org/10.1002/jlb.61.3.233
  22. Mori A, Zhai YL, Toki T, Nikaido T, Fujii S (1997) Distribution and heterogeneity of mast cells in the human uterus. Hum Reprod 12(2):368-372. https://doi.org/10.1093/humrep/12.2.368
  23. Nawa Y, Horii Y, Okada M, Arizono N(1994) Histochemical and cytological characterizations of mucosal and connective tissue mast cells of Mongolian gerbils (Meriones unguiculatus). Int Arch Allergy Immunol 104(3):249-254. https://doi.org/10.1159/000236673
  24. Noviana D, Kono F, Nagakui Y, Shimizu H, Mamba K, Makimurra S, Horii Y (2001) Distribution and enzyme histochemical characterisation of mast cells in cats. Histochem J 33(11-12):597-603. https://doi.org/10.1023/A:1016324515108
  25. Noviana D, Mamba K, Makimura S, Horii Y (2004) Distribution, histochemical and enzyme histochemical characterization of mast cells in dogs. J Mol Histol 35(2):123-132.
  26. Padilla L, Reinicke K, Montesino H, Villena F, Asencio H, Cruz M, Rudolph Mi (1990) Histamine content and mast cells distribution in mouse uterus: The effect of sexual hormones, gestation and labor. Cellular and Molecular Biol 36(1):93-100.
  27. Parshad RK, Kathpalia K (1990) Distribution and characteristics of mast cells in the rat ovary during the oestrous cycle, lactation and pregnancy. Folia Morphol (Praha) 38(4):327-330.
  28. Rudolph MI, Reinicke K, Cruz MA, Gonzalez C, Bardisa L (1993) Distribution of mast cells and the effect of their mediators on contractility in human myometrium. Br J Obstet Gynecol 100:1125-1130. https://doi.org/10.1111/j.1471-0528.1993.tb15178.x
  29. Rudolph MI, Rojas IG, Penissi AB(2004) Uterine mast cells: A new hypothesis to understand how we are born. Biocell 28(1):1-11.
  30. Rumbolz WL, Greene EG(1957) Observations on metachromatic granules in human endometrium. Am J Obstet Gynecol. 73(5):992-997; discussion, 998-999. https://doi.org/10.1016/S0002-9378(16)37170-8
  31. Sasaki Y, Yoshimoto T, Maruyama H, Tegoshi T, Ohta N, Arizono N, Nakanishi K (2005) IL-18 with IL-2 protects against Strongyloides venezuelensis infection by activating mucosal mast cell-dependent type 2 innate immunity. J Exp Med 5;202(5):607-616. https://doi.org/10.1084/jem.20042202
  32. Schwartz LB (1998) The mast cell, in Textbook of Rheumatology (Kelley WN, Harris ED, Ruddy S, Sledge CB, eds.), W.B. Saunders Company, Philadelphia, pp. 161-175.
  33. Sivridis E, Giatromanolaki A, Agnantis N, Anastasiadis P (2001) Mast cell distribution and density in the normal uterus-metachromatic staining using lectins. Eur J Obstet Gynecol Reprod Biol 98(1):109-113. https://doi.org/10.1016/S0301-2115(00)00564-9
  34. Tainsh KR, Pearce FL (1992) Mast cell heterogeneity: evidence that mast cells isolated from various connective tissue locations in the rat display markedly graded phenotypes. Int Arch Allergy Immunol 98(1): 26-34. https://doi.org/10.1159/000236161
  35. Varayoud J, Ramos JG, Bosquiazzo VL, Munoz-de-Toro M, Luque EH (2004) Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy. Reproduction 127(3):379-387. https://doi.org/10.1530/rep.1.00018
  36. Vliagoftis H, Metcalfe DD (1997) Characterization of adhesive interactions between mast cells and laminin isoforms: Evidence of a principal role for alpha 6 integrin. Immunology 92(4):553-560. https://doi.org/10.1046/j.1365-2567.1997.00368.x
  37. Walter J, Klein C, Wehrend A (2011) Distribution of mast cells in vaginal cervical and uterine tissue of nonpregnant mares: Investigations on correlations with ovarian steroids. Reprod Domest Anim. doi: 10.1111/ j.1439-0531.2011.01897.x. [Epub ahead of print].
  38. Waynforth HB (1980) Experimental and Surgical Technique in the Rat. Published A subsidiary of Harcourt Brace Fovanovich, London.
  39. 김형민 (1998) 면역과 알레르기. 신일상사.
  40. 최영자, 제갈승주, 이철상, 김재만 (2008) 생쥐 자궁조직 내 비만세포의 연령별 밀도 및 분포 변화. Dev Repord 12(3):243-250.