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황사 발생 기간 낙하먼지에 포함된 미생물의 분포 및 특성

Distribution and Characteristics of Microorganisms Associated with Settled Particles During Asian Dust Events

  • 고지윤 (울산대학교 자연과학대학 생명과학부) ;
  • 장찬국 (울산대학교 자연과학대학 생명과학부) ;
  • 차민주 (울산대학교 자연과학대학 생명과학부) ;
  • 박교남 (울산대학교 자연과학대학 생명과학부) ;
  • 김민규 (울산대학교 자연과학대학 생명과학부) ;
  • 김종설 (울산대학교 자연과학대학 생명과학부)
  • Koh, Ji-Yun (Department of Biological Science, University of Ulsan) ;
  • Jang, Chan-Gook (Department of Biological Science, University of Ulsan) ;
  • Cha, Min-Ju (Department of Biological Science, University of Ulsan) ;
  • Park, Kyo-Nam (Department of Biological Science, University of Ulsan) ;
  • Kim, Min-Kyu (Department of Biological Science, University of Ulsan) ;
  • Kim, Jong-Seol (Department of Biological Science, University of Ulsan)
  • 투고 : 2012.06.05
  • 심사 : 2012.06.27
  • 발행 : 2012.06.30

초록

황사는 중국과 몽골의 사막지역과 중국 황하 중 상류의 황토지대에서 바람에 의해 대기 중에 부유한 미세먼지가 상층 바람을 타고 멀리까지 날아가는 기상 현상을 말한다. 황사 먼지는 bioaerosol을 포함하며 미생물의 운반체로 작용할 수 있다. 따라서 본 연구에서는 황사 발생 기간과 황사가 없는 기간 동안 각각 포집한 낙하먼지에서 미생물의 농도와 종 조성을 비교하고자 하였다. 2008년 2월부터 4월까지 울산 지역의 한 지점에서 직경 200 mm의 강수량계를 사용하여 낙하먼지를 포집하였으며, 조사 기간 동안 울산 지역에서 황사 현상은 3월 2일과 3일에 걸쳐 한번 발생하였다. 낙하먼지에 포함된 세균의 농도는, 황사 기간 동안 포집한 시료에서 황사가 없는 기간의 시료에 비해 크게 높았다. 하지만, 낙하먼지의 진균 농도는 비 황사 기간에 비해 황사 기간 동안 유의성 있게 증가하지 않았으며, 조사 기간 동안 세균에 비해 상대적으로 일정한 수준을 유지하였다. 낙하먼지 시료로부터 분리한 45개 세균의 16S rRNA 유전자 염기 서열을 분석하였으며, 이들 세균은 Bacillus 속의 B. amyloliquefaciens, B. aryabhattai, B. atrophaeus, B. licheniformis, B. megaterium, B. methylotrophicus, B. pumilus, B. sonorensis, B. subtlis, B. vallismortis와 Staphylococcus 속의 S. epidermidis, S. succinus로 확인되었다. 진균의 경우 Mucor 속, Alternaria 속, Cladosporium 속, Aspergillus 속 등을 황사 기간 및 비 황사 기간의 시료에서 모두 확인할 수 있었다. Bacillus 속과 같은 내생포자를 형성하는 세균이 진균(포자)에 비해 황사 먼지에의 부착 및 이동과 더 연관이 있는 것으로 보인다.

Asian dust storms originating in the arid desert of China and Mongolia usually occur from late winter through spring, and more than one million tons of dust per year is transported to the Korean Peninsula by the prevalent westerly winds. We supposed that these dust particles could include bioaerosols and act as carriers of microorganisms. In order to clarify the dynamics of microorganisms moving with these particles, the concentration and composition of microorganisms associated with settled particles were compared between samples collected during Asian dust events and those under non-dust periods. From February to April 2008, settled dust particles were collected at one location in Ulsan using rainfall meter of 200 mm diameter. During this period, there was one Asian dust event in Ulsan. The bacterial concentrations were higher in samples collected during Asian dust event than those under non-dust period, whereas fungal concentrations were rather similar regardless of the Asian dust event. We analyzed 16S rRNA gene sequences of 45 bacterial isolates obtained from the settled particle samples. These isolates belonged to either genus Bacillus or genus Streptococcus and were tentatively identified as B. amyloliquefaciens, B. aryabhattai, B. atrophaeus, B. licheniformis, B. megaterium, B. methylotrophicus, B. pumilus, B. sonorensis, B. subtlis, B. vallismortis, S. epidermidis, and S. succinus. In cases of fungal isolates, genera such as Mucor, Alternaria, Cladosporium, and Aspergillus were tentatively identified from samples collected at both Asian dust and non-Asian dust periods. It appears that endospore-forming bacteria such as Bacillus sp. rather than fungal spores are more likely to be associated with Asian dust particles.

키워드

참고문헌

  1. American public health association. 1996. Standard methods for the examination of water and wastewater, 19th ed. American Public Health Association, Washington, D.C., USA.
  2. Atlas, R.M. and Parks, L.C. 1996. Handbook of microbiological media. CRC press, Boca Raton, Florida, USA.
  3. Bae, K.W., Kim, J.H., Kim, Y.S., Park, J.S., Jee, Y.K., and Lee, K.Y. 2005. Microbiological identification and distribution of metal components in suspended particulate matter during yellow sand phenomena at Taean region in 2003. Tuber. Respir. Dis. 58, 167-173. https://doi.org/10.4046/trd.2005.58.2.167
  4. Choi, D.-S., Park, Y.-K., Oh, S.-K., Yoon, H.-J., Kim, J.C., Seo, W.-J., and Cha, S.-H. 1997. Distribution of airborne microorganisms in yellow sands of Korea. J. Microbiol. 35, 1-9.
  5. Chun, Y., Boo, K.-O., Kim, J., Park, S.-U., and Lee, M. 2001. Synopsis, transport, and physical characteristics of Asian dust in Korea. J. Geophy. Res. 106, 18461-18469. https://doi.org/10.1029/2001JD900184
  6. Fang, Z., Ouyang, Z., Hu, L., Wang, X., Zheng, H., and Lin, X. 2005. Culturable airborne fungi in outdoor environments in Beijing, China. Sci. Total Environ. 350, 47-58. https://doi.org/10.1016/j.scitotenv.2005.01.032
  7. Fang, Z., Ouyang, Z., Zheng, H., Wang, X., and Hu, L. 2007. Culturable airborne bacteria in outdoor environments in Beijing, China. Microb. Ecol. 54, 487-496. https://doi.org/10.1007/s00248-007-9216-3
  8. Griffin, D.W. 2007. Atmospheric movement of microorganisms in clouds of desert dust and implications for human health. Clin. Microbiol. Rev. 20, 459-477. https://doi.org/10.1128/CMR.00039-06
  9. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95-98.
  10. Ichinose, T., Yoshida, S., Hiyoshi, K., Sadakane, K., Takano, H., Nishikawa, M., More, I., Yanagisawa, R., Kawazato, H., Yasuda, A., and et al. 2008. The effects of microbial materials adhered to Asian sand dust on allergic lung inflammation. Arch. Environ. Contam. Toxicol. 55, 348-357. https://doi.org/10.1007/s00244-007-9128-8
  11. Iwasaka, Y., Shi, G.-Y., Yamada, M., Kobayashi, F., Kakikawa, M., Maki, T., Naganuma, T., Chen, B., Tobo, Y., and Hong, C.S. 2009. Mixture of Kosa (Asian dust) and bioaerosols detected in the atmosphere over the Kosa particles source regions with balloon-borne measurements: possibility of long-range transport. Air Qual. Atmos. Health 2, 29-38. https://doi.org/10.1007/s11869-009-0031-5
  12. Korea meteorological administration. 2012. Available at http://www.kma.go.kr/weather/asiandust/density.jsp.
  13. Lane, D.J. 1991. 16S/23S rRNA sequencing, pp. 115-175. In Stackebrandt, E. and Goodfellow, M. (eds.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Chichester, UK.
  14. Lee, S., Choi, B., Yi, S.-M., and Ko, G. 2009. Characterization of microbial community during Asian dust events in Korea. Sci. Total Environ. 407, 5308-5314. https://doi.org/10.1016/j.scitotenv.2009.06.052
  15. Lee, J.-T., Son, J.-Y., and Cho, Y.-S. 2007. A comparison of mortality related to urban air particles between periods with Asian dust days and without Asian dust days in Seoul, Korea, 2000-2004. Environ. Res. 105, 409-413. https://doi.org/10.1016/j.envres.2007.06.004
  16. Pace, N.R. 1997. A molecular view of microbial diversity and the biosphere. Science 276, 734. https://doi.org/10.1126/science.276.5313.734
  17. Park, E.-J., Kim, D.-S., and Park, K. 2008. Monitoring of ambient particles and heavy metals in a residential area of Seoul, Korea. Environ. Monit. Assess. 137, 441-449. https://doi.org/10.1007/s10661-007-9779-y
  18. Park, J.W., Lim, Y.H., Kyung, S.Y., An, C.H., Lee, S.P., and Jeong, S.H. 2005. Detection of pathogenic viruses in the atmosphere during Asian dust events in Incheon city. Tuber. Respir. Dis. 59, 279-285. https://doi.org/10.4046/trd.2005.59.3.279
  19. Samson, R.A., Hoekstra, E.S., Frisvad, J.C., and Filtenborg, O. 2002. Introduction to food- and airborne fungi, 6th edition. Centraalbureau voor Schimmelcultures, Utrecht, Netherlands.
  20. Shelton, B.G., Kirkland, K.H., and Flanders, W.D. 2002. Profiles of airborne fungi in buildings and outdoor environments in the United States. Appl. Environ. Microbiol. 68, 1743-1753. https://doi.org/10.1128/AEM.68.4.1743-1753.2002
  21. Watanabe, T. 1994. Pictorial atlas of soil and seed fungi-morphology of cultured fungi and key to species. CRC press, Boca Raton, Florida, USA.
  22. Yeo, H.-G. and Kim, J.-H. 2002. SPM and fungal spores in the ambient air of west Korea during the Asian dust (Yellow sand) period. Atmos. Environ. 36, 5437-5442. https://doi.org/10.1016/S1352-2310(02)00672-6
  23. Yoo, Y., Choung, J.T., Yu, J., Kim, D.K., and Koh, Y.Y. 2008. Acute effects of Asian dust events on respiratory symptoms and peak expiratory flow in children with mild asthma. J. Korean Med. Sci. 23, 66-71. https://doi.org/10.3346/jkms.2008.23.1.66

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  2. 24절기 야외 부유곰팡이 농도 조사 및 기상요소와의 상관성 분석 vol.35, pp.6, 2019, https://doi.org/10.12654/jcs.2019.35.6.08