Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Functional Analysis of spy1 Responsible for Pristinamycin Yield in Streptomyces pristinaespiralis

  • Jin, Qingchao (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University) ;
  • Yin, Huali (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University) ;
  • Hong, Xiaowei (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University) ;
  • Jin, Zhihua (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University)
  • Received : 2011.11.11
  • Accepted : 2012.02.06
  • Published : 2012.06.28
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Abstract

A gene related to high pristinamycin yield in Streptomyces pristinaespiralis was selected by amplified fragment length polymorphism (AFLP) and its functions were investigated by gene disruption. First, a 561 bp polymorphic sequence was acquired by AFLP from high-yield recombinants compared with the S. pristinaespiralis ancestor ATCC25486, indicating that this approach is an effective means of screening for valuable genes responsible for antibiotic yield. Then, a 2,127 bp open reading frame of a gene designated spy1 that overlaps with the above fragment was identified and its structure and biological functions were investigated. In silico analysis of spy1 encoding a deduced 708-amino-acid-long serine/threonine protein kinase showed that it only contains a catalytic domain in the N-terminal region, which is different from some known homologs. Gene inactivation of chromosomal spy1 indicated that it plays a pleiotropic regulatory function in pristinamycin production, with a positive correlation to pristinamycin I biosynthesis and a negative correlation to pristinamycin II biosynthesis.

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References

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