Journal of Microbiology and Biotechnology

  • 제22권6호
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  • Pages.793-799
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation and Functional Analysis of spy1 Responsible for Pristinamycin Yield in Streptomyces pristinaespiralis

  • Jin, Qingchao (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University) ;
  • Yin, Huali (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University) ;
  • Hong, Xiaowei (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University) ;
  • Jin, Zhihua (School of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University)
  • 투고 : 2011.11.11
  • 심사 : 2012.02.06
  • 발행 : 2012.06.28
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초록

A gene related to high pristinamycin yield in Streptomyces pristinaespiralis was selected by amplified fragment length polymorphism (AFLP) and its functions were investigated by gene disruption. First, a 561 bp polymorphic sequence was acquired by AFLP from high-yield recombinants compared with the S. pristinaespiralis ancestor ATCC25486, indicating that this approach is an effective means of screening for valuable genes responsible for antibiotic yield. Then, a 2,127 bp open reading frame of a gene designated spy1 that overlaps with the above fragment was identified and its structure and biological functions were investigated. In silico analysis of spy1 encoding a deduced 708-amino-acid-long serine/threonine protein kinase showed that it only contains a catalytic domain in the N-terminal region, which is different from some known homologs. Gene inactivation of chromosomal spy1 indicated that it plays a pleiotropic regulatory function in pristinamycin production, with a positive correlation to pristinamycin I biosynthesis and a negative correlation to pristinamycin II biosynthesis.

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참고문헌

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피인용 문헌

  1. Probing the Molecular Mechanisms for Pristinamycin Yield Enhancement in Streptomyces pristinaespiralis vol.65, pp.6, 2012, https://doi.org/10.1007/s00284-012-0233-1
  2. Streptogramins - Two are better than one! vol.304, pp.1, 2012, https://doi.org/10.1016/j.ijmm.2013.08.008
  3. Involvement of the TetR-Type Regulator PaaR in the Regulation of Pristinamycin I Biosynthesis through an Effect on Precursor Supply in Streptomyces pristinaespiralis vol.197, pp.12, 2012, https://doi.org/10.1128/jb.00045-15
  4. Identification of two novel regulatory genes involved in pristinamycin biosynthesis and elucidation of the mechanism for AtrA-p-mediated regulation in Streptomyces pristinaespiralis vol.99, pp.17, 2015, https://doi.org/10.1007/s00253-015-6638-6
  5. A Complex Signaling Cascade Governs Pristinamycin Biosynthesis in Streptomyces pristinaespiralis vol.81, pp.19, 2012, https://doi.org/10.1128/aem.00728-15
  6. Improvement of pristinamycin I (PI) production in Streptomyces pristinaespiralis by metabolic engineering approaches vol.2, pp.2, 2017, https://doi.org/10.1016/j.synbio.2017.06.001