Journal of Microbiology and Biotechnology

  • 제22권5호
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  • Pages.642-648
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Cloning and Characterization of the Orotidine-5'-Phosphate Decarboxylase Gene (URA3) from the Osmotolerant Yeast Candida magnoliae

  • Park, Eun-Hee (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Seo, Jin-Ho (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Myoung-Dong (School of Biotechnology and Bioengineering, Kangwon National University)
  • 투고 : 2011.11.28
  • 심사 : 2012.01.11
  • 발행 : 2012.05.28
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초록

We determined the nucleotide sequence of the URA3 gene encoding orotidine-5'-phosphate decarboxylase (OMPDCase) of the erythritol-producing osmotolerant yeast Candida magnoliae by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 795 bp, encoding a 264 amino acid residue protein with the highest identity to the OMPDCase of the yeast Kluyveromyces marxianus. Phylogenetic analysis of the deduced amino acid sequence revealed that it shared a high degree of identity with other yeast OMPDCase homologs. The cloned URA3 gene successfully complemented the ura3 null mutation in Saccharomyces cerevisiae, revealing that it encodes a functional OMPDCase in C. magnoliae. An enzyme activity assay and reverse transcription polymerase chain reaction indicated that the expression level of the C. magnoliae URA3 gene in S. cerevisiae was not as high as that of the S. cerevisiae URA3 gene. The GenBank accession number for C. magnoliae URA3 is JF521441.

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참고문헌

  1. Al-Fattani, M. A. and L. J. Douglas. 2006. Biofilm matrix of Candida albicans and Candida tropicalis: Chemical composition and role in drug resistance. J. Med. Microbiol. 55: 999-1008. https://doi.org/10.1099/jmm.0.46569-0
  2. Boeke, J. D., F. LaCroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-Fluoro-orotic acid resistance. Mol. Gen. Genet. 197: 345-346. https://doi.org/10.1007/BF00330984
  3. Crick, F. H. 1966. Codon-anticodon pairing: The wobble hypothesis. J. Mol. Biol. 19: 548-555. https://doi.org/10.1016/S0022-2836(66)80022-0
  4. Gietz, R. D. and R. A. Woods. 2002. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Meth. Enzymol. 350: 87-96.
  5. Guo, Z. and F. Sherman. 1995. 3'-End-forming signals of yeast mRNA. Mol. Cell. Biol. 15: 5983-5990.
  6. Harbury, P. and K. Struhl. 1989. Functional distinctions between yeast TATA elements. Mol. Cell. Biol. 9: 5298-5304.
  7. Healy, A. and R. Zitomer. 1990. A sequence that directs transcriptional initiation in yeast. Curr. Genet. 18: 105-109. https://doi.org/10.1007/BF00312597
  8. Hiele, M., Y. Ghoos, P. Rutgeerts, and G. Vantrappen. 1993. Metabolism of erythritol in humans: Comparison with glucose and lactitol. Br. J. Nutr. 69: 169-176. https://doi.org/10.1079/BJN19930019
  9. Kalpaxis, D., I. Zundorf, H. Werner, N. Reindl, E. Boy-Marcotte, M. Jacquet, and T. Dingermann. 1991. Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid. Mol. Gen. Genet. 225: 492-500.
  10. Kim, S. Y., Y. J. Jeon, and J. H. Seo. 1996. Analysis of fermentation characteristics for production of erythritol by Candida sp. Kor. J. Food Sci. Technol. 28: 935-939.
  11. Lee, D. H., M. D. Kim, Y. W. Ryu, and J. H. Seo. 2008. Cloning and characterization of CmGPD1, the Candida magnoliae homologue of glycerol-3-phosphate dehydrogenase. FEMS Yeast Res. 8: 1324-1333. https://doi.org/10.1111/j.1567-1364.2008.00446.x
  12. Lee, D. H., Y. J. Lee, Y. W. Ryu, and J. H. Seo. 2010. Molecular cloning and biochemical characterization of a novel erythrose reductase from Candida magnoliae JH110. Microb. Cell Fact. 9: 43. https://doi.org/10.1186/1475-2859-9-43
  13. Lee, W. J., M. D. Kim, Y. W. Ryu, L. F. Bisson, and J. H. Seo. 2002. Kinetic studies on glucose and xylose transport in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 60: 186-191. https://doi.org/10.1007/s00253-002-1085-6
  14. Li, Y., W. Shen, Z. Wang, J. Q. Liu, Z. Rao, X. Tang, et al. 2005. Isolation and sequence analysis of the gene URA3 encoding the orotidine-5'-phosphate decarboxylase from Candida glycerinogenes WL2002-5, an industrial glycerol producer. Yeast 22: 423-430. https://doi.org/10.1002/yea.1211
  15. Lloyd, A. T. and P. M. Sharp. 1992. Evolution of codon usage patterns: The extent and nature of divergence between Candida albicans and Saccharomyces cerevisiae. Nucleic Acids Res. 20: 5289-5295. https://doi.org/10.1093/nar/20.20.5289
  16. Makinen, K. K., M. Saag, K. P. Isotupa, J. Olak, R. Nommela, E. Soderling, and P. L. Makinen. 2005. Similarity of the effects of erythritol and xylitol on some risk factors of dental caries. Caries Res. 39: 207-215. https://doi.org/10.1159/000084800
  17. Miller, B. G., M. J. Snider, R. Wolfenden, and S. A. Short. 2001. Dissecting a charged network at the active site of orotidine-5'-phosphate decarboxylase. J. Biol. Chem. 276: 15174-15176. https://doi.org/10.1074/jbc.M011429200
  18. Mount, R. C., B. E. Jordan, and C. Hadfield. 1996. Transformation of lithium-treated yeast cells and the selection of auxotrophic and dominant markers. Methods Mol. Biol. 53: 139-145.
  19. Noda, K. and T. Oku. 1992. Metabolism and disposition of erythritol after oral administration to rats. J. Nutr. 122: 1266-1272.
  20. Park, J. M., N. S. Han, and T. J. Kim. 2007. Rapid detection and isolation of known and putative $\alpha$-L-arabinofuranosidase genes using degenerate PCR primers. J. Microbiol. Biotechnol. 17: 481-489.
  21. Park, S. Y., Y. W. Ryu, and J. H. Seo. 2003. Two-stage fed-batch culture of Candida magnoliae for the production of erythritol using an industrial medium. Kor. J. Biotechnol. Bioeng. 18: 249-254.
  22. Reinoso, C., F. Sorais, G. A. Nino-Vega, E. Ferminan, G. San-Blas, and A. Dominguez. 2005. Cloning and functional analysis of the orotidine-5'-phosphate decarboxylase gene (PbrURA3) of the pathogenic fungus Paracoccidioides brasiliensis. Yeast 22: 739-743. https://doi.org/10.1002/yea.1248
  23. Rose, M. and D. Botstein. 1983. Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast. J. Mol. Biol. 170: 883-904. https://doi.org/10.1016/S0022-2836(83)80193-4
  24. Rose, M., P. Grisafi, and D. Botstein. 1984. Structure and function of the yeast URA3 gene: Expression in Escherichia coli. Gene 29: 113-124. https://doi.org/10.1016/0378-1119(84)90172-0
  25. Sakai, Y., T. Kazarimoto, and Y. Tani. 1991. Transformation system for an asporogenous methylotrophic yeast, Candida boidinii: Cloning of the orotidine-5'-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation. J. Bacteriol. 173: 7458-7463.
  26. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning, pp. 1.119-1.122, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  27. Takahashi, S., R. Matsunaga, Y. Kera, and R. H. Yamada. 2003. Isolation of the Cryptococcus humicolus URA3 gene encoding orotidine-5'-phosphate decarboxylase and its use as a selective marker for transformation. J. Biosci. Bioeng. 96: 23-31.
  28. Tsang, P. W., B. Cao, P. Y. Siu, and J. Wang. 1999. Loss of heterozygosity, by mitotic gene conversion and crossing over, causes strain-specific adenine mutants in constitutive diploid Candida albicans. Microbiology 145: 1623-1629. https://doi.org/10.1099/13500872-145-7-1623
  29. Turakainen, H. and M. Korhola. 2005. Cloning, sequencing and application of the LEU2 gene from the sour dough yeast Candida milleri. Yeast 22: 805-812. https://doi.org/10.1002/yea.1246
  30. Umezu, K., T. Amaya, A. Yoshimoto, and K. Tomita. 1971. Purification and properties of orotidine-5'-phosphate pyrophosphorylase and orotidine-5'-phosphate decarboxylase from baker's yeast. J. Biochem. 70: 249-262.
  31. Van Bogaert, I. N. A., S. L. D. Maeseneire, W. D. Schamphelaire, D. Develter, W. Soetaert, and E. J. Vandamme. 2007. Cloning, characterization and functionality of the orotidine-5'-phosphate decarboxylase gene (URA3) of the glycolipid-producing yeast Candida bombicola. Yeast 24: 201-208. https://doi.org/10.1002/yea.1448
  32. Yu, J. H., D. H. Lee, Y. C. Park, M. G. Lee, D. O. Kim, Y. W. Ryu, and J. H. Seo. 2008. Proteomic analysis of fructophilic properties of osmotolerant Candida magnoliae. J. Microbiol. Biotechnol.18: 248-254.
  33. Zhong, W., M. W. Jeffries, and N. H. Georgopapadakou. 2000. Inhibition of inositol phosphorylceramide synthase by aureobasidin A in Candida and Aspergillus species. Antimicrob. Agents Chemother. 44: 651-653. https://doi.org/10.1128/AAC.44.3.651-653.2000

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