Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

6-Methoxyluteolin from Chrysanthemum zawadskii var. latilobum Suppresses Histamine Release and Calcium Influx via Down-Regulation of $Fc{\varepsilon}RI$ ${\alpha}$ Chain Expression

  • Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University) ;
  • Park, Jeong-Ro (Department of Food and Nutrition, Sunchon National University) ;
  • Byun, Dae-Seok (Department of Food Science and Nutrition, Pukyong National University)
  • Received : 2011.11.25
  • Accepted : 2012.01.02
  • Published : 2012.05.28
Download PDF
⟨ Previous Next ⟩

Abstract

Mast cells and basophils are important effector cells in immunoglobulin-E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-methoxyluteolin, isolated from Chrysanthemum zawadskii, in the $Fc{\varepsilon}RI$-mediated allergic reaction. We determined that 6-methoxyluteolin inhibited anti-$Fc{\varepsilon}RI$ ${\alpha}$ chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration $[Ca^{2+}]_i$ in a dose-dependent manner. Moreover, the inhibitory effects of 6-methoxyluteolin on the cell surface expression and the mRNA level of the $Fc{\varepsilon}RI$ ${\alpha}$ chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Therefore, these results show that 6-methoxyluteolin is a potent inhibitor of histamine release and calcium influx via down-regulation of the $Fc{\varepsilon}RI$ ${\alpha}$ chain.

Keywords

References

  1. Nolte, H., V. Backer, and C. Porsbjerg. 2001. Environmental factors as a cause for the increase in allergic disease. Ann. Allergy Asthma Immunol. 87: 7-11. https://doi.org/10.1016/S1081-1206(10)62333-2
  2. Beaven, M. A. and H. Metzer. 1993. Signal transduction by Fc receptors: The $Fc{\varepsilon}RI$ case. Immunol. Today 14: 222-226. https://doi.org/10.1016/0167-5699(93)90167-J
  3. Drombrowicz, D., V. Flamand, K. K. Brigman, B. H. Koller, and J. P. Koller. 1993. Abolition of anaphylaxis by targeted disruption of the high affinity immunoglobulin E receptor $\varepsilon$ chain gene. Cell 75: 969-976. https://doi.org/10.1016/0092-8674(93)90540-7
  4. Kinet, J. P., U. Blank, A. Brini, M. H. Jouvin, H. Kuster, O. Mejan, and C. Ra. 1991. The high affinity receptor for immunoglobulin E: A target for therapy of allergic diseases. Int. Arch. Allergy Appl. Immunol. 94: 51-55. https://doi.org/10.1159/000235323
  5. Kinet, J. P. 1999. The high affinity IgE receptor ($Fc{\varepsilon}RI$): From physiology to pathology. Annu. Rev. Immunol. 17: 931-972. https://doi.org/10.1146/annurev.immunol.17.1.931
  6. Gauchat, J. F., S. Henchoz, G. Mazzel, J. P. Aubry, T. Brunner, H. Blasey, et al. 1993. Induction of human IgE synthesis in B cells by mast cells and basophils. Nature 365: 340-343. https://doi.org/10.1038/365340a0
  7. Metzer, H. 1991. The high affinity receptor for IgE on mast cells. Clin. Exp. Immunol. 4: 269-279.
  8. Hakimi, J. C., J. A. Seals, L. Kondas, W. Pettine, W. Danko, and J. Kochan. 1990. The a subunit of the human IgE receptor ($Fc{\varepsilon}RI$) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081.
  9. Kwon, H. S., T. J. Ha, S. W. Hwang, Y. M. Jin, S. H. Nam, H. K. Park, and M. S. Yang. 2006. Cytotoxic flavonoids from the whole plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura. J. Life Sci. 16: 746-749. https://doi.org/10.5352/JLS.2006.16.5.746
  10. Seo, J. Y., S. S. Lim, J. A. Park, J. S. Lim, H. J. Kim, H. J. Kang, et al. 2010. Protection by Chrysanthemum zawadskii extract from liver damage of mice caused by carbon tetrachloride is maybe mediated by modulation of QR activity. Nutr. Res. Pract. 4: 93-98. https://doi.org/10.4162/nrp.2010.4.2.93
  11. Singh, R. P., P. Agrawal, D. S. Yim, C. Agarwal, and R. Agarwal. 2005. Acacetin inhibits cell growth and cell cycle progression, and induces apoptosis in human prostate cancer cells: Structure-activity relationship with linarin and linarin acetate. Carcinogenesis 26: 845-854. https://doi.org/10.1093/carcin/bgi014
  12. Kim, Y. Y., S. Y. Lee, and D. S. Yim. 2001. Biological activities of linarin from Chrysanthemum zawadskii var. latilobum. J. Pharm. Soc. Korea 45: 604-610.
  13. Hsu, Y. L., P. L. Kuo, and C. C. Lin. 2004. Acacetin inhibitis the proliferation of Hep G2 by blocking cell cycle progression and inducing apoptosis. Biochem. Pharmacol. 67: 823-829. https://doi.org/10.1016/j.bcp.2003.09.042
  14. Kim, Y. Y., S. Y. Lee, and D. S. Yim. 2001. Biological activities of linarin from Chrysanthemum zawadskii var. latilobum. Yakhak Hoeji 45: 604-610.
  15. Han, S., K. H. Sung, D. Yim, S. Lee, C. K. Lee, N. J. Ha, and K. Kim. 2002. The effect of linarin on LPS-induced cytokine production and nitric oxide inhibition in murine macrophage cell line RAW264.7. Arch. Pharm. Res. 25: 170-177. https://doi.org/10.1007/BF02976559
  16. Wu, T. Y., T. O. Khor, C. L. Saw, S. C. Loh, A. I. Chen, S. S. Lim, et al. 2010. Anti-inflammatory/anti-oxidative stress activities and differential regulation of Nrf2-mediated genes by non-polar fractions of tea Chrysanthemum zawadskii and licorice Glycyrrhiza uralensis. AAPS J. 13: 1-13.
  17. Fujimura, Y., H. Tachibana, and K. Yamada. 2001. A tea catechin suppresses the expression of the high-affinity IgE receptor $Fc{\varepsilon}RI$ in human basophilic KU812 cells. J. Agric. Food Chem. 49: 2527-2531. https://doi.org/10.1021/jf001392w
  18. Fujimura, Y., H. Tachibana, M. Maeda-Yamamoto, T. Miyase, M. Sano, and K. Yamada. 2002. Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses $Fc{\varepsilon}RI$ expression in human basophilic KU812 cells. J. Agric. Food Chem. 50: 5729-5734. https://doi.org/10.1021/jf025680z
  19. Li, Y., S. H. Lee, Q. T. Le, M. M. Kim, and S. K. Kim. 2008. Anti-allergic effects of phlorotannins on histamine release via binding inhibition between IgE and $Fc{\varepsilon}RI$. J Agric. Food Chem. 56: 12073-12080. https://doi.org/10.1021/jf802732n
  20. Shim, S. Y., J. S. Choi, and D. S. Byun. 2009. Kaempferol isolated from Nelumbo nucifera stamens negatively regulates FcepsilonRI expression in human basophilic KU812F cells. J. Microbiol. Biotechnol. 19: 155-160. https://doi.org/10.4014/jmb.0804.259
  21. Shim, S. Y., J. S. Choi, and D. S. Byun. 2009. Inhibitory effects of phloroglucinol derivatives isolated from Ecklonia stolonifera on Fc(epsilon)RI expression. Bioorg. Med. Chem. 17: 4734-4739. https://doi.org/10.1016/j.bmc.2009.04.050
  22. Shim, S. Y. and D. S. Byun. 2011. Inhibitory effects of Chrysanthemum zawadsaki ethanolic extract on $Fc{\varepsilon}RI$ $\alpha$ chain expression. Korean J. Food Sci. Technol. 43: 220-223.
  23. Shore, P., A. A. Burkhalter, V. H. Cohn, and H. C. Victor. 1959. A method for the fluorometric assay of histamine in tissues. J. Pharmacol. Exp. Ther. 127: 182-186.
  24. Dinda, B., B. C. Mohanta, S. Arima, N. Sato, and Y. Harigawa. 2007. Flavonoids from the stem-bark of Oroxylum indicum. Nat. Prod. Sci. 13: 190-194.
  25. Hakimi, J. C., J. A. Seals, L. Kondas, W. Pettine, W. Danko, and J. Kochan. 1990. The $\alpha$ subunit of the human IgE receptor ($Fc{\varepsilon}RI$) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081.
  26. Blank, U., C. Ra, L. Miller, K. White, H. Metzer, and J. P. Kinet. 1989. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 337: 187-189. https://doi.org/10.1038/337187a0
  27. Miller, L., U. Blank, H. Metzer, and J. P. Kinet. 1989. Expression of high affinity binding of human immunoglobulin E by transfected cells. Science 244: 334-337. https://doi.org/10.1126/science.2523561
  28. Ra, C., M. H. Jouvin, and J. P. Kinet. 1989. Complete structure of the mouse mast cell receptor for IgE ($Fc{\varepsilon}RI$) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells. J. Biol. Chem. 264: 15323-15327.
  29. Wu, L. C. 2011. Immunoglobulin E receptor signaling and asthma. J. Biol. Chem. 286: 32891-32897. https://doi.org/10.1074/jbc.R110.205104

Cited by

  1. Mast cells in meningiomas and brain inflammation vol.12, pp.None, 2015, https://doi.org/10.1186/s12974-015-0388-3
  2. Brain “fog,” inflammation and obesity: key aspects of neuropsychiatric disorders improved by luteolin vol.9, pp.None, 2012, https://doi.org/10.3389/fnins.2015.00225
  3. The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells vol.135, pp.4, 2015, https://doi.org/10.1016/j.jaci.2014.10.032
  4. Isolation of Luteolin and Luteolin-7-O-glucoside from Dendranthema morifolium Ramat Tzvel and Their Pharmacokinetics in Rats vol.63, pp.35, 2012, https://doi.org/10.1021/jf505848z
  5. Sesquiterpene Lactones and Flavonoids from Psephellus pyrrhoblepharus with Antiproliferative Activity on Human Gynecological Cancer Cell Lines vol.24, pp.17, 2019, https://doi.org/10.3390/molecules24173165