Journal of Microbiology and Biotechnology

  • 제22권4호
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  • Pages.530-534
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Development of Real-Time PCR for the Detection of Clostridium perfringens in Meats and Vegetables

  • Chon, Jung-Whan (KU Center for Food Safety, Veterinary Science Research Institute and College of Veterinary Medicine, Konkuk University) ;
  • Park, Jong-Seok (Division of Research Planning and Management, Korea Food and Drug Administration) ;
  • Hyeon, Ji-Yeon (KU Center for Food Safety, Veterinary Science Research Institute and College of Veterinary Medicine, Konkuk University) ;
  • Park, Chan-Kyu (Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University) ;
  • Song, Kwang-Young (KU Center for Food Safety, Veterinary Science Research Institute and College of Veterinary Medicine, Konkuk University) ;
  • Hong, Kwang-Won (Department of Food Science and Technology, Dongguk University) ;
  • Hwang, In-Gyun (Division of Microbiology, Korea Food and Drug Administration) ;
  • Kwak, Hyo-Sun (Division of Foodborne Diseases Prevention and Surveillance, Korea Food and Drug Administration) ;
  • Seo, Kun-Ho (KU Center for Food Safety, Veterinary Science Research Institute and College of Veterinary Medicine, Konkuk University)
  • 투고 : 2011.07.29
  • 심사 : 2011.12.07
  • 발행 : 2012.04.28
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초록

A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was $10^2$ CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.

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참고문헌

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