Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Bupleurum falcatum Prevents Depression and Anxiety-Like Behaviors in Rats Exposed to Repeated Restraint Stress

  • Lee, Bom-Bi (Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University) ;
  • Yun, Hye-Yeon (The Graduate School of Basic Science of Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Shim, In-Sop (Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Hye-Jung (Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University) ;
  • Hahm, Dae-Hyun (Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University)
  • Received : 2011.10.26
  • Accepted : 2011.11.17
  • Published : 2012.03.28
Download PDF
⟨ Previous Next ⟩

Abstract

Previous studies have demonstrated that repeated restraint stress in rodents produces increases in depression and anxiety-like behaviors and alters the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. The current study focused on the impact of Bupleurum falcatum (BF) extract administration on repeated restraint stress-induced behavioral responses using the forced swimming test (FST) and elevated plus maze (EPM) test. Immunohistochemical examinations of tyrosine hydroxylase (TH) expression in rat brain were also conducted. Male rats received daily doses of 20, 50, or 100 mg/kg (i.p.) BF extract for 15 days, 30 min prior to restraint stress (4 h/day). Hypothalamic-pituitary-adrenal axis activation in response to repeated restraint stress was confirmed base on serum corticosterone levels and CRF expression in the hypothalamus. Animals that were pre-treated with BF extract displayed significantly reduced immobility in the FST and increased open-arm exploration in the EPM test in comparison with controls. BF also blocked the increase in TH expression in the locus coeruleus of treated rats that experienced restraint stress. Together, these results demonstrate that BF extract administration prior to restraint stress significantly reduces depression and anxiety-like behaviors, possibly through central adrenergic mechanisms, and they suggest a role for BF extract in the treatment of depression and anxiety disorders.

Keywords

References

  1. Bravo, J. A., G. Diaz-Veliz, S. Mora, J. L. Ulloa, V. M. Berthoud, P. Morales. et al. 2009. Desipramine prevents stressinduced changes in depressive-like behavior and hippocampal markers of neuroprotection. Behav. Pharmacol. 20: 273-285. https://doi.org/10.1097/FBP.0b013e32832c70d9
  2. Checkley, S. 1996. The neuroendocrinology of depression and chronic stress. Br. Med. Bull. 52: 597-617. https://doi.org/10.1093/oxfordjournals.bmb.a011570
  3. Chotiwat, C. and R. B. Harris. 2008. Antagonism of specific corticotropin-releasing factor receptor subtypes selectively modifies weight loss in restrained rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: R1762-R1773. https://doi.org/10.1152/ajpregu.00196.2008
  4. Chotiwat, C., E. W. Kelso, and R. B. Harris. 2010. The effects of repeated restraint stress on energy balance and behavior of mice with selective deletion of CRF receptors. Stress 13: 203-213. https://doi.org/10.3109/10253890903207527
  5. Cryan, J. F. and A. Holmes. 2005. The ascent of mouse: Advances in modelling human depression and anxiety. Nat. Rev. Drug Discov. 4: 775-790. https://doi.org/10.1038/nrd1825
  6. Culman, J., I. J. Kopin, and J. M. Saavedra. 1991. Regulation of corticotropin-releasing hormone and pituitary-adrenocortical response during acute and repeated stress in the rat. Endocr. Regul. 25: 151-158.
  7. Dhabhar, F. S., B. S. McEwen, and R. L. Spencer. 1997. Adaptation to prolonged or repeated stress - comparison between rat strains showing intrinsic differences in reactivity to acute stress. Neuroendocrinology 65: 360-368. https://doi.org/10.1159/000127196
  8. Dunn, A. J. and C. W. Berridge. 1990. Physiological and behavioral responses to corticotrophin-releasing factor administration: Is CRF a mediator of anxiety or stress responses? Brain Res. Rev. 15: 71-100. https://doi.org/10.1016/0165-0173(90)90012-D
  9. Fan, J. M., X. Q. Chen, H. Jin, and J. Z. Du. 2009. Gestational hypoxia alone or combined with restraint sensitizes the hypothalamic-pituitary-adrenal axis and induces anxiety-like behavior in adult male rat offspring. Neuroscience 10: 1363-1373.
  10. Gregus, A., A. J. Wintink, A. C. Davis, and L. E. Kalynchuk. 2005. Effect of repeated corticosterone injections and restraint stress on anxiety and depression-like behavior in male rats. Behav. Brain Res. 156: 105-114. https://doi.org/10.1016/j.bbr.2004.05.013
  11. Harris, R. B., J. Zhou, B. D. Youngblood, I. I. Rybkin, G. N. Smagin, and D. H. Ryan. 1998. Effect of repeated stress on body weight and body composition of rats fed low- and high-fat diets. Am. J. Physiol. 275: R1928-R1938.
  12. Kessler, R. C., J. Soukup, R. B. Davis, D. F. Foster, S. A. Wilkey, M. I. Van Rompay, and D. M. Eisenberg. 2001. The use of complementary and alternative therapies to treat anxiety and depression in the United States. Am. J. Psychiatry 158: 289-294. https://doi.org/10.1176/appi.ajp.158.2.289
  13. Koshikawa, N., T. Imai, I. Takahashi, M. Yamauchi, S. Sawada, and A. Kansaku. 1998. Effects of Hochuekki-to, Yoku-kan-san and Saiko-ka-ryukotsu-borei-to on behavioral despair and acetic acid-induced writhing in mice. Methods Find. Exp. Clin. Pharmacol. 20: 47-51. https://doi.org/10.1358/mf.1998.20.1.485631
  14. Kwon, S., B. Lee, M. Kim, H. Lee, H. J. Park, and D. H. Hahm. 2009. Antidepressant-like effect of the methanolic extract from Bupleurum falcatum in the tail suspension test. Prog. Neuro. Psychopharmacol. Biol. Psychiatry 34: 265-270.
  15. Lee, B., I. Shim, H. Lee, and D. H. Hahm. 2009. Effect of Bupleurum falcatum on the stress-induced impairment of spatial working memory in rats. Biol. Pharm. Bull. 32: 1392-1398. https://doi.org/10.1248/bpb.32.1392
  16. Liu, J., J. C. Garza, J. Bronner, C. S. Kim, W. Zhang, and X. Y. Lu. 2010. Acute administration of leptin produces anxiolyticlike effects: A comparison with fluoxetine. Psychopharmacol. (Berl.) 207: 535-545. https://doi.org/10.1007/s00213-009-1684-3
  17. Lucki, I. 1997. The forced swimming test as a model for core and component behavioral effects of antidepressant drugs. Behav. Pharmacol. 8: 523-532. https://doi.org/10.1097/00008877-199711000-00010
  18. McEwen, B. S. 2000. The neurobiology of stress: From serendipity to clinical relevance. Brain Res. 886: 172-189. https://doi.org/10.1016/S0006-8993(00)02950-4
  19. Millan, M. J. 2009. Dual- and triple-acting agents for treating core and co-morbid symptoms of major depression: Novel concepts, new drugs. Neurotherapeutics 6: 53-77. https://doi.org/10.1016/j.nurt.2008.10.039
  20. Mineka, S., D. Watson, and A. L. Clark. 1998. Comorbidity of anxiety and unipolar mood disorders. Annu. Rev. Psychol. 49: 377-412. https://doi.org/10.1146/annurev.psych.49.1.377
  21. Mineka, S. and R. Zinbarg. 2006. A contemporary learning theory perspective on the etiology of anxiety disorders: It's not what you thought it was. Am. Psychol. 61: 10-26.
  22. Mizoguchi, K., N. Sun, X. L. Jin, Y. Kase, S. Takeda, W. Maruyama, and T. Tabira. 2007. Saikokaryukotsuboreito, a herbal medicine, prevents chronic stress-induced dysfunction of glucocorticoid negative feedback system in rat brain. Pharmacol. Biochem. Behav. 86: 55-61. https://doi.org/10.1016/j.pbb.2006.12.007
  23. Nemeroff, C. B. 1998. The neurobiology of depression. Sci. Am. 278: 42-49.
  24. Nestler, E. J., M. Barrot, R. J. DiLeone, A. J. Eisch, S. J. Gold, and L. M. Monteggia. 2002. Neurobiology of depression. Neuron 34: 13-25. https://doi.org/10.1016/S0896-6273(02)00653-0
  25. Osterhout, C. A., C. R. Sterling, D. M. Chikaraishi, and A. W. Tank. 2005. Induction of tyrosine hydroxylase in the locus coeruleus of transgenic mice in response to stress or nicotine treatment: Lack of activation of tyrosine hydroxylase promoter activity. J. Neurochem. 94: 731-741. https://doi.org/10.1111/j.1471-4159.2005.03222.x
  26. Padovan, C. M. and F. S. Guimaraes. 2000. Restraint-induced hypoactivity in an elevated plus-maze. Braz. J. Med. Biol. Res. 33: 79-83.
  27. Park, H. J., H. S. Shim, H. Kim, K. S. Kim, H. Lee, D. H. Hahm, and I. Shim. 2010. Effects of Glycyrrhizae Radix on repeated restraint stress-induced neurochemical and behavioral responses. Korean J. Physiol. Pharmacol. 14: 371-376. https://doi.org/10.4196/kjpp.2010.14.6.371
  28. Paxinos, G. and C. Watson. 1986. The Rat Brain in Stereotaxic Coordinates. New York, Academic Press.
  29. Porsolt, R. D., G. Anton, N. Blavet, and M. Jalfre. 1978. Behavioral despair in rats: A new model sensitive to antidepressant treatments. Eur. J. Pharmacol. 15: 379-391.
  30. Sandi, C., J. J. Merino, M. I. Cordero, K. Touyarot, and C. Venero. 2001. Effects of chronic stress on contextual fear conditioning and the hippocampal expression of the neural cell adhesion molecule, ITS polysialylation and L1. Neuroscience 102: 329-339. https://doi.org/10.1016/S0306-4522(00)00484-X
  31. Sevgi, S., M. Ozek, and L. Eroglu. 2003. L-NAME prevents anxiety-like and depression-like behavior in rats exposed to restraint stress. Methods Find. Exp. Clin. Pharmacol. 28: 95-99.
  32. Strausbaugh, H. J., M. F. Dallman, and J. D. Levine. 1999. Repeated, but not acute, stress suppresses inflammatory plasma extravasation. Proc. Natl. Acad. Sci. USA 96: 14629-14634. https://doi.org/10.1073/pnas.96.25.14629
  33. Strohle, A. and F. Holsboer. 2003. Stress responsive neurohormones in depression and anxiety. Pharmacopsychiatry 3: S207-S214.
  34. Suvrathan, A., A. Tomar, and S. Chattarji. 2010. Effects of chronic and acute stress on rat behaviour in the forced-swim test. Stress 13: 533-540.
  35. Takagi, K. and M. Shibata. 1969. Pharmacological studies on Bupleurum falcatum L. II. Antiinflammatory and other pharmacological actions of crude saikosides. Yakugaku Zasshi 89: 1367-1378.
  36. Tsigos, C. and G. P. Chrousos. 2002. Hypothalamic-pituitary-adrenal axis, neuroendocrine factors and stress. J. Psychosom. Res. 53: 865-871. https://doi.org/10.1016/S0022-3999(02)00429-4
  37. Ulloa, J. L., P. Castaneda, C. Berrios, G. Diaz-Veliz, S. Mora, J. A. Bravo, et al. 2010. Comparison of the antidepressant sertraline on differential depression-like behaviors elicited by restraint stress and repeated corticosterone administration. Pharmacol. Biochem. Behav. 97: 213-221. https://doi.org/10.1016/j.pbb.2010.08.001
  38. Vyas, A., R. Mitra, B. S. Rao, and S. Chattarji. 2002. Chronic stress induces contrasting patterns of dendritic remodeling in hippocampal and amygdaloid neurons. J. Neurosci. 2: 6810-6818.
  39. Walf, A. A. and C. A. Frye. 2010. Estradiol reduces anxietyand depression-like behavior of aged female mice. Physiol. Behav. 99: 169-174. https://doi.org/10.1016/j.physbeh.2009.09.017
  40. Walf, A. A. and C. A. Frye. 2007. The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat. Protoc. 2: 322-328. https://doi.org/10.1038/nprot.2007.44
  41. Wang, R., Y. Xu, H. L. Wu, Y. B. Li, Y. H. Li, J. B. Guo, and X. J. Li. 2008. The antidepressant effects of curcumin in the forced swimming test involve 5-HT1 and 5-HT2 receptors. Eur. J. Pharmacol. 578: 43-50. https://doi.org/10.1016/j.ejphar.2007.08.045
  42. Wong, M. L. and J. Licinio. 2001. Research and treatment approaches to depression. Nat. Rev. Neurosci. 2: 343. https://doi.org/10.1038/35072566
  43. Yamada, K. and S. Kanba. 2002. Herbal medicine (kami-shoyosan) in the treatment of premenstrual dysphoric disorder. J. Clin. Psychopharmacol. 22: 442. https://doi.org/10.1097/00004714-200208000-00023
  44. Zhang, Z. J. 2004. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci. 75: 1659-1699. https://doi.org/10.1016/j.lfs.2004.04.014
  45. Zhang, L. D., Y. L. Zhang, S. H. Xu, G. Zhou, and S. B. Jin. 1994. Traditional Chinese medicine typing of affective disorders and treatment. Am. J. Chin. Med. 22: 321-327. https://doi.org/10.1142/S0192415X94000383

Cited by

  1. A Valid Approach in Refractory Glossodynia: A Single-Institution 5-Year Experience Treating with Japanese Traditional Herbal (Kampo) Medicine vol.2013, pp.None, 2012, https://doi.org/10.1155/2013/354872
  2. Screening of Stat3 inhibitory effects of Korean herbal medicines in the A549 human lung cancer cell line vol.3, pp.2, 2014, https://doi.org/10.1016/j.imr.2013.10.004
  3. 산후 우울증 치료에 대한 중의학의 최근 임상연구 동향 vol.25, pp.2, 2012, https://doi.org/10.7231/jon.2014.25.2.179
  4. Possible Involvement of µ Opioid Receptor in the Antidepressant-Like Effect of Shuyu Formula in Restraint Stress-Induced Depression-Like Rats vol.2015, pp.None, 2012, https://doi.org/10.1155/2015/452412
  5. Comparison of root transcriptomes and expressions of genes involved in main medicinal secondary metabolites from Bupleurum chinense and Bupleurum scorzonerifolium, the two Chinese official Radix buple vol.153, pp.2, 2012, https://doi.org/10.1111/ppl.12254
  6. An Oriental Medicine, Hyungbangpaedok-San Attenuates Motor Paralysis in an Experimental Model of Multiple Sclerosis by Regulating the T Cell Response vol.10, pp.10, 2012, https://doi.org/10.1371/journal.pone.0138592
  7. The Complete Chloroplast Genome of the Hare’s Ear Root, Bupleurum falcatum : Its Molecular Features vol.7, pp.5, 2016, https://doi.org/10.3390/genes7050020
  8. The Role of MAPK and Dopaminergic Synapse Signaling Pathways in Antidepressant Effect of Electroacupuncture Pretreatment in Chronic Restraint Stress Rats vol.2017, pp.None, 2017, https://doi.org/10.1155/2017/2357653
  9. A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications vol.55, pp.1, 2012, https://doi.org/10.1080/13880209.2016.1262433
  10. Treatment with Shuyu capsule increases 5-HT1AR level and activation of cAMP-PKA-CREB pathway in hippocampal neurons treated with serum from a rat model of depression vol.17, pp.3, 2012, https://doi.org/10.3892/mmr.2017.8339
  11. Protective effects of a traditional herbal extract from Stellaria dichotoma var. lanceolata against Mycobacterium abscessus infections vol.13, pp.11, 2012, https://doi.org/10.1371/journal.pone.0207696
  12. Kamikihito Enhances Cognitive Functions and Reward-Related Behaviors of Aged C57BL/6J Mice in an Automated Behavioral Assay System vol.11, pp.None, 2012, https://doi.org/10.3389/fphar.2020.01037
  13. The complete mitochondrial genome sequences of Bupleurum falcatum (Apiales: Apiaceae) vol.5, pp.3, 2012, https://doi.org/10.1080/23802359.2020.1781566
  14. 한약처방 JSB가 구금스트레스로 우울증을 유발한 생쥐의 행동과 생체지표에 미치는 영향 vol.32, pp.1, 2021, https://doi.org/10.7231/jon.2021.32.1.025
  15. Medicinal Plants in the Treatment of Depression: Evidence from Preclinical Studies vol.87, pp.9, 2012, https://doi.org/10.1055/a-1338-1011