Journal of Microbiology and Biotechnology

  • 제22권1호
  • /
  • Pages.92-99
  • /
  • 2012
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Poly(L-Lactide)-Degrading Enzyme Production by Actinomadura keratinilytica T16-1 in 3 L Airlift Bioreactor and Its Degradation Ability for Biological Recycle

  • 투고 : 2011.05.12
  • 심사 : 2011.10.03
  • 발행 : 2012.01.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The optimal physical factors affecting enzyme production in an airlift fermenter have not been studied so far. Therefore, the physical parameters such as aeration rate, pH, and temperature affecting PLA-degrading enzyme production by Actinomadura keratinilytica strain T16-1 in a 3 l airlift fermenter were investigated. The response surface methodology (RSM) was used to optimize PLA-degrading enzyme production by implementing the central composite design. The optimal conditions for higher production of PLA-degrading enzyme were aeration rate of 0.43 vvm, pH of 6.85, and temperature at $46^{\circ}C$. Under these conditions, the model predicted a PLA-degrading activity of 254 U/ml. Verification of the optimization showed that PLA-degrading enzyme production of 257 U/ml was observed after 3 days cultivation under the optimal conditions in a 3 l airlift fermenter. The production under the optimized condition in the airlift fermenter was higher than un-optimized condition by 1.7 folds and 12 folds with un-optimized medium or condition in shake flasks. This is the first report on the optimization of environmental conditions for improvement of PLA-degrading enzyme production in a 3 l airlift fermenter by using a statistical analysis method. Moreover, the crude PLA-degrading enzyme could be adsorbed to the substrate and degraded PLA powder to produce lactic acid as degradation products. Therefore, this incident indicates that PLA-degrading enzyme produced by Actinomadura keratinilytica NBRC 104111 strain T16-1 has a potential to degrade PLA to lactic acid as a monomer and can be used for the recycle of PLA polymer.

키워드

참고문헌

  1. Anbu, P., S. C. B. Gopinath, A. Hilda, T. Lakshmipriya, and G. Annadurai. 2007. Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis. Bioresour. Technol. 98: 1298-1303. https://doi.org/10.1016/j.biortech.2006.05.047
  2. Box, G. E. P. and K. B. Wilson. 1951. On the experimental attainment of optimum conditions. J. Roy. Stat. Soc. B 13: 1-45.
  3. Jarerat, A. and Y. Tokiwa. 2001. Degradation of poly(L-lactide) by a fungus. Macromol. Biosci. 1: 136-140. https://doi.org/10.1002/1616-5195(20010601)1:4<136::AID-MABI136>3.0.CO;2-3
  4. Jarerat, A., Y. Tokiwa, and H. Tanaka. 2004. Microbial poly(Llactide)- degrading enzyme induced by amino acids, peptides, and poly(L-amino acids). J. Polym. Eviron. 12: 139-146. https://doi.org/10.1023/B:JOOE.0000038545.69235.f2
  5. Jarerat, A., Y. Tokiwa, and H. Tanaka. 2006. Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide). Appl. Microbiol. Biotechnol. 72: 726-731. https://doi.org/10.1007/s00253-006-0343-4
  6. Kim, M. N., W. G. Kim, H. Y. Weon, and S. H. Lee. 2007. Poly(L-lactide)-degrading activity of a newly isolated bacterium. J. Appl. Polym. Sci. 109: 234-239.
  7. Li, Y., F. Cui, Z. Liu, Y. Xu, and H. Zhao. 2007. Improvement of xylanase production by Penicillium oxalicum ZH-30 using response surface methodology. Enzyme Microb. Technol 40: 1381-1388. https://doi.org/10.1016/j.enzmictec.2006.10.015
  8. Myers, R. H. and D. C. Montgomery. 2002. Response Surface Methodology: Process and Product Optimization Using Designed Experiments, 2nd Ed. John Wiley and Sons, Inc.
  9. Naidu, K. S. B. and K. L. Devi. 2005. Optimization of thermostable alkaline protease production from species of Bacillus using rice bran. Afr. J. Biotechnol. 4: 724-726. https://doi.org/10.5897/AJB2005.000-3132
  10. Qadar, S. A. U., E. Shireen, S. Iqbal, and A. Anwar. 2009. Optimization of protease production from newly isolated strain of Bacillus sp. PCSIR EA-3. Indian J. Biotechnol. 8: 286-290.
  11. Sakai, K., H. Kawano, A. Iwami, M. Nakamura, and M. Moriguchi. 2001. Isolation of a thermophilic poly-L-lactide degrading bacterium from compost and its enzymatic characterization. J. Biosci. Bioeng. 92: 298-300. https://doi.org/10.1016/S1389-1723(01)80266-8
  12. Singh, R. S., B. S. Sooch, and M. Puri. 2007. Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1. Bioresour. Technol. 98: 2518-2525. https://doi.org/10.1016/j.biortech.2006.09.011
  13. Sukkhum, S., S. Tokuyama, T. Tamura, and V. Kitpreechavanich. 2009. A novel poly(L-lactide) degrading actinomycetes isolated from Thai forest soils, phylogenic relationship and enzyme characterization. J. Gen. Appl. Microbiol. 55: 459-467. https://doi.org/10.2323/jgam.55.459
  14. Sukkhum, S., S. Tokuyama, and V. Kitpreechavanich. 2009. Development of fermentation process for PLA-degrading enzyme production by a new thermophilic Actinomadura sp. T16-1. Biotechnol. Bioprocess Eng. 14: 302-306. https://doi.org/10.1007/s12257-008-0207-0
  15. Tanyildizi, M. S., D. Ozer, and M. Elibol. 2005. Optimization of $\alpha$-amylase production by Bacillus sp. using RSM. Process Biochem. 40: 2291-2296. https://doi.org/10.1016/j.procbio.2004.06.018
  16. Tomita, K., Y. Kuroki, and K. Nakai. 1999. Isolation of thermophiles degrading poly(L-lactic acid). J. Biosci. Bioeng. 87: 752-755. https://doi.org/10.1016/S1389-1723(99)80148-0
  17. Tomita, K., T. Nakajima, Y. Kikuchi, and N. Miwa. 2004. Degradation of poly(L-lactic acid) by a newly isolated thermophile. Polym. Degrad. Stab. 84: 433-438. https://doi.org/10.1016/j.polymdegradstab.2003.12.006
  18. Tsuji, H. and K. Nakahara. 2002. Poly(L-lactide) IX. Hydrolysis in acid media. J. Appl. Polym. Sci. 86: 186-194. https://doi.org/10.1002/app.10813

피인용 문헌

  1. Production of Poly(l-Lactide)-Degrading Enzyme by Amycolatopsis orientalis ssp. orientalis and Its Catalytic Ability in Biological Recycling of Poly(l-Lactide) vol.21, pp.4, 2012, https://doi.org/10.1007/s10924-013-0590-2
  2. Characterization on Biodegradation of Enzymatically Synthesized Polylactic Acid by Using Alkaline Protease and Lipase vol.29, pp.2, 2012, https://doi.org/10.3139/217.2835
  3. Thermophilic and alkaliphilic Actinobacteria : biology and potential applications vol.6, pp.None, 2012, https://doi.org/10.3389/fmicb.2015.01014
  4. Production of raw starch degrading enzyme by the thermophilic filamentous bacterium Laceyella sacchari LP175 and its application for ethanol production from dried cassava chips vol.68, pp.11, 2012, https://doi.org/10.1002/star.201600018
  5. Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization vol.25, pp.4, 2012, https://doi.org/10.1007/s10924-016-0885-1
  6. Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches vol.47, pp.7, 2012, https://doi.org/10.1080/10826068.2017.1315597
  7. Review on the current status of polymer degradation: a microbial approach vol.4, pp.None, 2017, https://doi.org/10.1186/s40643-017-0145-9
  8. Catalysis as an Enabling Science for Sustainable Polymers vol.118, pp.2, 2018, https://doi.org/10.1021/acs.chemrev.7b00329
  9. Isolation and Characterization of Polyester-Based Plastics-Degrading Bacteria from Compost Soils vol.87, pp.2, 2012, https://doi.org/10.1134/s0026261718020157
  10. Actinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation vol.10, pp.None, 2012, https://doi.org/10.3389/fmicb.2019.02834
  11. Poly(l-lactide)-Degrading Enzyme Production by Laceyella sacchari LP175 Under Solid State Fermentation Using Low Cost Agricultural Crops and Its Hydrolysis of Poly(l-lactide) Film vol.11, pp.5, 2012, https://doi.org/10.1007/s12649-018-0519-z
  12. Production of poly (l-lactide)-degrading enzyme by Actinomadura keratinilytica strain T16-1 under solid state fermentation using agricultural wastes as substrate vol.11, pp.12, 2012, https://doi.org/10.1007/s13205-021-03060-8