Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cells Transformed by PLC-Gamma 1 Overexpression are Highly Sensitive to Clostridium difficile Toxin A-Induced Apoptosis and Mitotic Inhibition

  • Nam, Hyo-Jung (Department of Life Science, College of Natural Science, Daejin University) ;
  • Kang, Jin-Ku (Department of Life Science, College of Natural Science, Daejin University) ;
  • Chang, Jong-Soo (Department of Life Science, College of Natural Science, Daejin University) ;
  • Lee, Min-Soo (Department of Life Science, College of Natural Science, Daejin University) ;
  • Nam, Seung-Taek (Department of Life Science, College of Natural Science, Daejin University) ;
  • Jung, Hyun-Woo (Department of Life Science, College of Natural Science, Daejin University) ;
  • Kim, Sung-Kuk (Department of Life Science, College of Natural Science, Daejin University) ;
  • Ha, Eun-Mi (College of Pharmacy, Catholic University of Daegu) ;
  • Seok, Heon (Department of Medical Engineering, Jungwon University) ;
  • Son, Seung-Woo (Suzhou Singapore International School, Suzhou Industrial Park) ;
  • Park, Young-Joo (Department of Internal Medicine, Seoul National University College of Medicine) ;
  • Kim, Ho (Department of Life Science, College of Natural Science, Daejin University)
  • Received : 2011.07.11
  • Accepted : 2011.09.23
  • Published : 2012.01.28
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Abstract

Phospholipase C-${\gamma}l$ (PLC-${\gamma}l$) expression is associated with cellular transformation. Notably, PLC-${\gamma}$ is up-regulated in colorectal cancer tissue and breast carcinoma. Because exotoxins released by Clostridium botulinum have been shown to induce apoptosis and promote growth arrest in various cancer cell lines, we examined here the potential of Clostridium difficile toxin A to selectively induce apoptosis in cells transformed by PLC-${\gamma}l$ overexpression. We found that PLC-${\gamma}l$-transformed cells, but not vector-transformed (control) cells, were highly sensitive to C. difficile toxin A-induced apoptosis and mitotic inhibition. Moreover, expression of the proapoptotic Bcl2 family member, Bim, and activation of caspase-3 were significantly up-regulated by toxin A in PLC-${\gamma}l$-transformed cells. Toxin A-induced cell rounding and paxillin dephosphorylation were also significantly higher in PLC-${\gamma}l$-transformed cells than in control cells. These findings suggest that C. difficile toxin A may have potential as an anticancer agent against colorectal cancers and breast carcinomas in which PLC-${\gamma}l$ is highly up-regulated.

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