대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제38권2호
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  • Pages.171-179
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  • 2012
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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항균활성을 갖는 천연물 개발과 화장품 응용에 관한 연구

Development of Antimicrobial Plant Extracts and its Application to Cosmetics

  • 이지영 (코리아나 화장품 송파기술연구소) ;
  • 이정노 (코리아나 화장품 송파기술연구소) ;
  • 이강태 (코리아나 화장품 송파기술연구소) ;
  • 이건국 (코리아나 화장품 송파기술연구소)
  • 투고 : 2012.04.04
  • 심사 : 2012.06.15
  • 발행 : 2012.06.30
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초록

본 연구는 항균 활성을 지니는 식물추출물을 찾고 화장품에서 방부제로서 적용하기 위해 진행되었다. 디스크 확산법(disk diffusion method)을 통해 3가지 식물 추출물, 함박꽃나무(천녀목란, Magnolia sieboldii), 오배자(Rhus chinensis), 메타세콰이어(Metasequioa glyptostroboides)가 항균활성을 지니고 있음을 확인하였다. 세 물질의 최소저해농도(MIC)를 측정한 결과 메타세콰이어는 0.3 ~ 0.35 %, 함박꽃나무는 0.35 ~ 0.4 % 농도에서 곰팡이의 생장을 억제하고 오배자는 0.45 ~ 0.5 % 농도에서 세균의 생장을 억제함을 확인하였다. 또한 추출물 내의 항균 활성을 지니는 성분을 분리하여 분석한 결과 메타세콰이어에서 분리한 caryophyllene oxide와 caryophyllene, 함박꽃나무에서 분리한 costunolide와 dehydrocostus lactone, 오배자에서 분리한 ethyl gallate, ethyl-3-gallate 등이 항균활성을 지닌 물질임을 확인하였다. O/W 에멀션 제형에서 식물 추출물을 넣고 방부력을 확인한 결과 혼합사용 시 세균과 곰팡이 모두에 대한 방부효과 가 있음을 확인하였다. 따라서 함박꽃나무, 오배자, 메타세콰이어 추출물의 혼합물은 기존의 화학 방부제를 대체할 천연 방부제로서 화장품에서 응용할 수 있을 것으로 기대된다.

This study is focused on finding new natural materials that have antimicrobial activity. We found that 3 plants extracts, Magnolia sieboldii K. Koch (M. sieboldii K. Koch), Rhus chinensis (R. chinensis) and Metasequioa glyptostroboides (M. glyptostroboides), have antimicrobial activities by disk diffusion method. We determined the Minimum Inhibitory Concentation (MIC) of each and found that 0.3 ~ 0.35 % of M. glyptostroboides essential oil and 0.35 ~ 0.4 % of M. sieboldii K. Koch extracts inhibited fungal growth and 0.45 ~ 0.5 % of R. chinensis extracts inhibited bacterial growth. We isolated compounds from extracts and verified what have antimicrobial activity. As a result we found that caryophyllene oxide and caryophyllene isolated from M. glyptostroboides, costunolide and dehydrocostus lactone from M. sieboldii K. Koch and ethyl gallate, ethyl-3-gallate from R. chinensis have antimicrobial activities. In accordance with antimicrobial activity, O/W cosmetic emulsion containing mixture of 3 plants extracts showed preservative efficacy against both bacteria and fungi. Based on the above data we suggest that extracts from M. siebodii K. Koch, R. chinensis, and M. glyptostroboides, replace chemical synthetic preservatives and be applied as a natural preservative.

키워드

참고문헌

  1. A. C. Groot and I. R. White, Cosmetics and skin care products, Textbook of Contact Dermatitis, 2nd Edition, 461 (1995).
  2. G. Robert, G. Jay, and V. Guenter, A review of the endocrine activity of parabens and implications for potential risks to human health, Critical Reviews in Toxicology, 35(5), 435 (2005). https://doi.org/10.1080/10408440490920104
  3. A. Varvaresou, E. Tsirivas, and E, Tsaoula, Isothiazolinone biocides as preservatives in cosmetics, Review of Clinical Pharmacology and Pharmacokinetics, 19, 105 (2005).
  4. E. Reinhard, R. Waeber, M. Niederem, T. Mauer, P. Maly, and S. Sherer, Preservation of products with MCI/MI in Switzerland, Contact Dermatitis, 45(5), 257 (2001). https://doi.org/10.1034/j.1600-0536.2001.450501.x
  5. A. Varvaresou, S. Papageorgiou, E. Tsirivas, H. Protopapa, H. Kintziou, V. Kefala, and C. Demetzos, Self preserving cosmetics, International Journal of Cosmetics Science, 31, 163 (2009). https://doi.org/10.1111/j.1468-2494.2009.00492.x
  6. J. H. Park, J. S. Lee, E. S. Jung, Y. M. Park, K. H. Kim, B. H. Park, K. S. Jung, E. K. Park, J. E. Kim, and D. H. Park, In vitro antibacterial and anti-inflammatory effects of honokiol and magnolol against Propionibacterium sp., European Journal of Pharmacology, 496(1), 189 (2004). https://doi.org/10.1016/j.ejphar.2004.05.047
  7. V. I. Chalova, P. G. Crandall, and S. C. Ricke, Microbial inhibitory and radical scavenging activities of cold-pressed terpeneless Valencia orange (Citrus-sinensis) oil in different dispersing agents, Journal of the Science Food and Agriculture, 90(5), 870 (2010).
  8. T. Mangena and N. Y. Muyima, Comparative evaluation of the antimicrobial activities of essential oils of Artemisiaafra, Pteroniaincana and Rosma-rinusofficinalis on selected bacteria and yeast strains. Letters in Applied Microbiology, 28(4), 291 (1999). https://doi.org/10.1046/j.1365-2672.1999.00525.x
  9. P. Lopez, C. Sanchez, R. Bettle, and C. Nerin, Development of flexible antimicrobial films using essential oils as active agents. Journal of Agricultural and Food Chemistry, 55(21), 8814 (2007). https://doi.org/10.1021/jf071737b
  10. G. Sing, S. Maurya, M. P. DeLampasona, and C. A. N. Cetalan, A comparison of chemical antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food and Chemical Toxicology, 45(9), 1650 (2007). https://doi.org/10.1016/j.fct.2007.02.031
  11. L. Pianizzi, G. Flamini, P. L. Cioni, and I. Morelli, Composition and antimicrobial properties of essential oils of four Mediterannean Lamiaceae, Journal of Ethnopharmacology, 39(3), 167 (1993). https://doi.org/10.1016/0378-8741(93)90032-Z
  12. F. Tian, B. Li, B. Ji, Z. Guizhi, and Y. Luo, Identification and structure-activity relationship of gallotannins separated from Galla chinensis, LWT - Food Science and Technology, 42(7), 1289 (2009). https://doi.org/10.1016/j.lwt.2009.03.004
  13. J. Zhang, L. Li, S. H. Kim, A. E. Hagerman, and J. Lu, Anti-cancer, anti-diabetic and other pharmacologic and biological activities of penta-galloylglucose, Parmaceutical Research, 26(9), 2066 (2009). https://doi.org/10.1007/s11095-009-9932-0
  14. M. M. Tarpay, D. F. Welch, and M. I. Marks, Antimicrobial susceptibility testing of Streptococcus pneumoniae by Micro-broth dilution, Antimicrobial agents and chemotherapy, 18(4), 579 (1980). https://doi.org/10.1128/AAC.18.4.579
  15. G. A. Jacoby and P. Han, Detection of extended-spectrum beta-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli, Journal of clinical microbiology, 34(4), 908 (1996).
  16. A. S. Curry, J. F. Graf, and G. N. McEwen, Determination of preservation adequacy of watermiscible cosmetic and toiletry formulations, CTFA Technical Guidelines: Microbiology Guidelines, M3, 1 (2001).

피인용 문헌

  1. Cudrania tricuspidata Root Extract as Whitening and Antiwrinkle Cosmetic Agent vol.52, pp.6, 2014, https://doi.org/10.9713/kcer.2014.52.6.701
  2. Development of a natural ingredient – Natural preservative: A case study vol.19, pp.9, 2016, https://doi.org/10.1016/j.crci.2016.06.004
  3. Antioxidant and Antimicrobial Activities of Combined Extracts of Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis vol.29, pp.1, 2014, https://doi.org/10.7841/ksbbj.2014.29.1.29
  4. Screening for Inhibition Activity of Plant Extracts on Microorganism Contaminating in Cosmetics vol.23, pp.1, 2015, https://doi.org/10.7783/KJMCS.2015.23.1.57
  5. Enzymatic Synthesis of 1, 2-Hexanediol Galactoside by Whole Cells of β-Galactosidase-containing Recombinant Escherichia coli vol.26, pp.5, 2016, https://doi.org/10.5352/JLS.2016.26.5.608