Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

Osteogenic Gene Expression on Anodizing Titanium Surface

양극산화 처리된 타이타늄 표면에서 골형성 유전자 발현

  • Kim, Won-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Kim, Young-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Jeon, Seong-Bae (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Jun, Sang-Ho (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Lee, Eui-Suk (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Jang, Hyon-Seok (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Kwon, Jong-Jin (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University) ;
  • Rim, Jae-Suk (Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University)
  • 김원석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 김영석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 전성배 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 전상호 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 이의석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 장현석 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 권종진 (고려대학교 의과대학 구강악안면외과학교실) ;
  • 임재석 (고려대학교 의과대학 구강악안면외과학교실)
  • Received : 2011.11.08
  • Accepted : 2012.03.26
  • Published : 2012.03.30
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Abstract

Purpose: The purpose of this study was to evaluate the expression of osteogenic genes associated with bone regeneration on anodizing titanium surface. Methods: $20{\times}20{\times}1$ (mm) commercially pure titanium plate was made, one group was pure titanium, second group was punched, and last group was punched and anodized by electrochemical method. Through the osteogenic cell culture model, the expression of extracellular matrix proteins, such as bone morphogenetic protein-2, bone sialoprotein, aggrecan, osteocalcin, Alkaline phosphatase, collagen I had been evaluated by Real-time polymerase chain reaction, and the morphology of growing cells was evaluated by scanning electron microscopy. Results: The attachment of mesenchymal stem cell was even and well-oriented on all Ti surfaces. The osteogene expression was increased on punching groups but, decreased on anodizing surfaces in 3 week samples. Conclusion: Punched anodizing Ti has possibility be using as a dental implant material, but further in vivo study would be needed.

Keywords

References

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