Archives of Plastic Surgery

  • 제39권2호
  • /
  • Pages.106-112
  • /
  • 2012
  • /
  • 2234-6163(pISSN)
  • /
  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지
DOI QR코드

DOI QR Code

The Effect of Platelet-rich Plasma on Wounds of OLETF Rats Using Expression of Matrix Metalloproteinase-2 and -9 mRNA

  • Shin, Ho-Seong (Department of Plastic and Reconstructive Surgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine) ;
  • Oh, Hwa-Young (Department of Plastic and Reconstructive Surgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine)
  • 투고 : 2011.10.01
  • 심사 : 2011.11.02
  • 발행 : 2012.03.15
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background : Complicated diabetic patients show impaired, delayed wound healing caused by multiple factors. A study on wound healing showed that platelet-rich plasma (PRP) was effective in normal tissue regeneration. Nonetheless, there is no evidence that when platelet-rich plasma is applied to diabetic wounds, it normalizes the diabetic wound healing process. In this study, we have analyzed matrix metalloproteinase (MMP)-2, MMP-9 expression to investigate the effect of PRP on diabetic wounds. Methods : Twenty-four-week-old male Otsuka Long-Evans Tokushima Fatty rats were provided by the Tokushima Research Institute. At 50 weeks, wounds were arranged in two sites on the lateral paraspinal areas. Each wound was treated with PRP gel and physiologic saline gauze. To determine the expression of MMP-2, MMP-9, which was chosen as a marker of wound healing, reverse transcription polymerase chain reaction (RT-PCR) was performed and local distribution and expression of MMP-2, MMP-9 was also observed throughout the immunohistochemical staining. Results : RT-PCR and the immunohistochemical study showed that the levels of MMP-2, MMP-9 mRNA expression in PRP applied tissues were higher than MMP-2, MMP-9 mRNA expression in saline-applied tissues. MMP-9 mRNA expression in wounds of diabetic rats decreased after healing began to occur. But no statistical differences were detected on the basis of body weight or fasting blood glucose levels. Conclusions : This study could indicate the extracellular matrix-regulating effect observed with PRP. Our results of the acceleration of wound healing events by PRP under hyperglycemic conditions might be a useful clue for future clinical treatment for diabetic wounds.

키워드

참고문헌

  1. Falanga V. Wound healing and its impairment in the diabetic foot. Lancet 2005;366:1736-43. https://doi.org/10.1016/S0140-6736(05)67700-8
  2. Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14:529-35.
  3. Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004;62:489-96. https://doi.org/10.1016/j.joms.2003.12.003
  4. Madlener M, Parks WC, Werner S. Matrix metalloproteinases (MMPs) and their physiological inhibitors (TIMPs) are differentially expressed during excisional skin wound repair. Exp Cell Res 1998;242:201-10. https://doi.org/10.1006/excr.1998.4049
  5. Kawano K, Hirashima T, Mori S, et al. Spontaneous longterm hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain. Diabetes 1992;41:1422-8. https://doi.org/10.2337/diabetes.41.11.1422
  6. Simman R, Hoffmann A, Bohinc RJ, et al. Role of plateletrich plasma in acceleration of bone fracture healing. Ann Plast Surg 2008;61:337-44. https://doi.org/10.1097/SAP.0b013e318157a185
  7. Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001;107:229-37. https://doi.org/10.1097/00006534-200101000-00037
  8. Della Valle A, Sammartino G, Marenzi G, et al. Prevention of postoperative bleeding in anticoagulated patients undergoing oral surgery: use of platelet-rich plasma gel. J Oral Maxillofac Surg 2003;61:1275-8. https://doi.org/10.1016/S0278-2391(03)00727-4
  9. Blanton MW, Hadad I, Johnstone BH, et al. Adipose stromal cells and platelet-rich plasma therapies synergistically increase revascularization during wound healing. Plast Reconstr Surg 2009;123:56S-64S. https://doi.org/10.1097/PRS.0b013e318191be2d
  10. Marx RE, Carlson ER, Eichstaedt RM, et al. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46. https://doi.org/10.1016/S1079-2104(98)90029-4
  11. Margolis DJ, Kantor J, Santanna J, et al. Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care 2001;24:483-8. https://doi.org/10.2337/diacare.24.3.483
  12. Medina A, Scott PG, Ghahary A, et al. Pathophysiology of chronic nonhealing wounds. J Burn Care Rehabil 2005;26: 306-19. https://doi.org/10.1097/01.BCR.0000169887.04973.3A
  13. Liu Y, Min D, Bolton T, et al. Increased matrix metalloproteinase- 9 predicts poor wound healing in diabetic foot ulcers. Diabetes Care 2009;32:117-9. https://doi.org/10.2337/dc08-0763
  14. Lan CC, Liu IH, Fang AH, et al. Hyperglycaemic conditions decrease cultured keratinocyte mobility: implications for impaired wound healing in patients with diabetes. Br J Dermatol 2008;159:1103-15.
  15. Lund LR, Romer J, Bugge TH, et al. Functional overlap between two classes of matrix-degrading proteases in wound healing. EMBO J 1999;18:4645-56. https://doi.org/10.1093/emboj/18.17.4645
  16. Wall SJ, Bevan D, Thomas DW, et al. Differential expression of matrix metalloproteinases during impaired wound healing of the diabetes mouse. J Invest Dermatol 2002;119:91-8. https://doi.org/10.1046/j.1523-1747.2002.01779.x

피인용 문헌

  1. Platelet-Rich Plasma and the Elimination of Neuropathic Pain vol.48, pp.2, 2012, https://doi.org/10.1007/s12035-013-8494-7
  2. Galectin-3 gene silencing inhibits migration and invasion of human tongue cancer cells in vitro via downregulating β-catenin vol.34, pp.1, 2012, https://doi.org/10.1038/aps.2012.150
  3. MMP inhibition as a potential method to augment the healing of skeletal muscle and tendon extracellular matrix vol.115, pp.6, 2012, https://doi.org/10.1152/japplphysiol.00137.2013
  4. 해조류 추출물의 발모효과에 관한 C57BL/6의 쥐 실험연구 vol.14, pp.1, 2012, https://doi.org/10.7181/acfs.2013.14.1.1
  5. Cell Therapy for Wound Healing vol.29, pp.3, 2012, https://doi.org/10.3346/jkms.2014.29.3.311
  6. Overview of Surgical Scar Prevention and Management vol.29, pp.6, 2012, https://doi.org/10.3346/jkms.2014.29.6.751
  7. Stem Cell and Research in Plastic Surgery vol.29, pp.suppl3, 2012, https://doi.org/10.3346/jkms.2014.29.s3.s167
  8. Effects of Polydeoxyribonucleotide in the Treatment of Pressure Ulcers vol.29, pp.suppl3, 2012, https://doi.org/10.3346/jkms.2014.29.s3.s222
  9. Use of Platelet-Rich Plasma to Reinforce the Staple Line During Laparoscopic Sleeve Gastrectomy: Feasibility Study and Preliminary Outcome vol.25, pp.3, 2012, https://doi.org/10.1089/lap.2014.0329
  10. The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous vol.2015, pp.None, 2012, https://doi.org/10.1155/2015/954701
  11. Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction vol.52, pp.2, 2015, https://doi.org/10.1007/s12035-015-9251-x
  12. Effect of platelet-rich plasma on the healing of cutaneous defects exposed to acute to chronic wounds: a clinico-histopathologic study in rabbits vol.10, pp.1, 2015, https://doi.org/10.1186/s13000-015-0327-8
  13. A comparative study between platelet-rich plasma and platelet-poor plasma effects on angiogenesis vol.51, pp.1, 2012, https://doi.org/10.1007/s00795-017-0168-5
  14. Investigation of the effects of thrombocyte-rich plasma, systemic ozone and hyperbaric oxygen treatment on intraoral wound healing in rats: experimental study vol.277, pp.6, 2012, https://doi.org/10.1007/s00405-020-05872-5
  15. Eliminating non-healing wounds: a review vol.16, pp.4, 2012, https://doi.org/10.2217/rme-2020-0163