Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Serum miR-21 Expression in Human Esophageal Squamous Cell Carcinomas

  • Cai, Er-Hui (Department of Preventive Medicine, Shantou University Medical College) ;
  • Gao, Yong-Xin (Department of Preventive Medicine, Shantou University Medical College) ;
  • Wei, Zhong-Zhi (Department of Preventive Medicine, Shantou University Medical College) ;
  • Chen, Wei-Ying (Center for Teaching Laboratories, Shantou University Medical College) ;
  • Yu, Ping (Department of Computers, Shantou University Medical College) ;
  • Li, Ke (Department of Preventive Medicine, Shantou University Medical College)
  • Published : 2012.04.30
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Abstract

To investigate the relationship between serum miRNA-21 (miR-21) expression in esophageal squamous cell carcinomas (ESCCs) and its clinicopathologic features, a 1:1 matched case-control study including 21 patients with ESCC and 21 age- and gender-matched healthy controls was carried out. Serum specimens were taken from all subjects. Total RNA was extracted and the stem-loop real time polymerase chain reaction was used to measure serum miR-21 in both groups. Clinical parameters were assessed to determine associations with serum miR-21 concentrations. Serum miR-21 expression in ESCC samples was significantly higher than in paired cancer-free samples (P<0.05). Metastasis was associated with mir-21 expression in serum (P<0.05), ESCC patients with metastasis having 8.4-fold higher serum miR-21 concentrations than healthy controls. There were no statistically significant associations between miR-21 expression and clinicopathologic parameters, such as gender (P>0.05), age (P>0.05), tumor location (P>0.05), cell differentiation (P>0.05), TNM staging (P>0.05), whether chemo/radiotherapy had been administered (P>0.05), or whether surgery had been performed (P>0.05). These findings suggest that the detection of microRNA-21 in serum might serve as a new tumor biomarker in diagnosis and assessment of prognosis of ESCCs.

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